Effects of Different Preparations of Gonadotropins (oFSH and pFSH) on Superovulatory Response and Embryo Yield in Indigenous Ewes in Bangladesh
محورهای موضوعی : CamelP.K. Jha 1 , M. Golam Shahi Alam 2 , M.R.I. Talukder 3 , F.Y. Bari 4
1 - Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh|Nepal Agricultural Research Council (NARC), Khumaltar, Lalitpur, Kathmandu, Nepal
2 - Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
3 - Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
4 - Department of Surgery and Obstetrics, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh, Bangladesh
کلید واژه: Sheep, corpus luteum, synchronization,
چکیده مقاله :
Two different gonadotropins on superovulatory response and embryo yield in indigenous ewes were studied. Donor ewes (n=33) were synchronized for oestrus using vaginal sponges containing 45 mg flurogestone acetate (FGA) for 12 days. For the superovulation, different types of gonadotropins were used in ewes randomly allocated into group I [n=16; ovine follicle-stimulating hormone (oFSH), 2.2 mg] and group II [n=17; porcine follicle-stimulating hormone (pFSH), 25 mg]. Gonadotropin treatments started on day 10 of FGA treatment for four consecutive days. The superovulatory response and embryo recovery was performed by modified semi-laparoscopic method on day 5/6 following mating. The stages (very early morula, morula, early blastocyst, blastocyst and hatching blastocyst) and grades (grade 1 to 5) of embryos were calculated. The oestrus response rate was 100%, and had significantly earlier onset of oestrus with pFSH than oFSH (23.41±0.50 h vs. 26.15±0.46 h, respectively). The corpus luteum/donor was significantly higher with pFSH than oFSH (14.18±1.63 vs. 10.25±0.89, respectively). The embryos recovered/donor differed insignificantly with pFSH than oFSH (11.82±1.48 vs. 8.81±0.86, respectively). The embryo recovery rate was higher using oFSH than pFSH (85.04±2.93% vs. 82.75±3.31%, respectively). The proportion of morula and grade 1 embryos was differed insignificantly with both oFSH and pFSH (86.52% and 97.16% vs. 84.58% and 90.55%, respectively). In conclusion, both the oFSH and pFSH were equally efficient, giving consistent ovulation rate and embryo yield.
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