Ileal Digestibility of Amino Acids in Fish Meal-Based Diets for Broiler Starters Using Regression Technique
محورهای موضوعی : Camel
آ.اف. آگبولا
1
(Department of Animal Science, University of Ibadan, Ibadan, Nigeria)
ای.آ. لیایی
2
(Department of Animal Science, University of Ibadan, Ibadan, Nigeria)
کلید واژه: Amino acids, broiler starters, fish meal, ileal digestibility, regression technique,
چکیده مقاله :
An experiment was conducted to determine apparent ileal digestibility of crude protein (CP) and amino acids (AA) and digestible CP and AA of fish meal (FM) in 21-d-old broiler chickens using the regression technique. Four diets containing 0, 1, 2, and 3% FM in place of cornstarch were formulated with FM as the sole contributor of CP and AAs in the sequential diets. Eighty 14-d-old broiler chicks were weighed, and sorted by body weight and randomly allotted to the experimental diets with 4 replicates per diet and 5 chicks per replicate. Titanium dioxide was added to the diets at the rate of 5 g/kg diet as an indigestible marker. They were fed the experimental diets till d 21 post hatch when they were killed by asphyxiated with CO2 and digesta from the last two-third of ileum collected. The concentrations of CP and AA in the diets increased as dietary FM increased. Apparent digestibility of CP and AA significantly (P<0.05) increased with dietary FM inclusion but with a decrease in the 3% FM diet. The digestibility values of CP, lysine and methionine estimated as slopes of the regression of the amount of CP or AA digested dry matter intake dry matter intake (DMI) at the terminal ileum against CP or AA intake dry matter intake (DMI) were 88.7, 97.2 and 97.0% respectively. The digestible amounts of CP, lysine and methionine of FM were 63.63 g, 5361.04 mg and 1801.45 mg. Results of the study suggest a maximum level of 2% inclusion of FM was adequate for broiler starter chicks as higher levels may induce higher endogenous CP and AA flow and hence reduced digestibility of these nutrients.
برای اندازه‌گیری قابلیت هضم ایلئومی ظاهری پروتئین‌خام و اسیدهای آمینه و پروتئین و اسیدهای آمینه قابل هضم پودر ماهی در جوجه‌های گوشتی 21 روزه با استفاده از روش تابعیت، آزمایشی انجام شد. چهار جیره که در آنها 0، 1، 2 و 3 درصد پودر ماهی جایگزین نشاسته ذرت شده بود فرموله شدند و پودر ماهی به عنوان تنها تأمین کننده پروتئین و اسیدهای آمینه در جیره‌ها بود. هشتاد قطعه جوجه گوشتی 14 روزه وزن شدند و براساس وزن بدن به طور تصادفی به جیره‌های آزمایشی با 4 تکرار و 5 جوجه در هر تکرار اختصاص یافتند. اکسید تیتانیوم به مقدار 5 g/kg به عنوان معرف غیرقابل هضم اضافه شد. جوجه‌ها تا 21 روزگی از جیره‌ها تغذیه و سپس با استفاده از دی‌اکسید‌کربن خفه شدند. محتویات روده از دو سوم انتهایی ایلئوم جمع‌آوری گردید. با استفاده از پودر ماهی، مقدار پروتئین و اسیدهای آمینه جیره‌ها افزایش یافتند. گرچه با افزودن پودر ماهی به جیره، به قابلیت هضم ظاهری پروتئین و اسیدهای آمینه اضافه شد (05/0>P) اما با 3 درصد پودر ماهی کاهش یافت. قابلیت هضم پروتئین، لیزین و متیونین به صورت شیب تابعیت پروتئین یا اسید آمینه هضم شده در ماده خشک مصرفی در انتهای ایلئوم در برابر پروتئین یا اسید آمینه هضم شده در ماده خشک مصرفی به ترتیب 7/88، 2/97 و 97 درصد تخمین زده شد. مقدار قابل هضم پروتئین، لیزین و متیونین پودر ماهی 63/63، 04/536 و 45/1801 میلی‌گرم بود. نتایج تحقیق پیشنهاد می‌کند حداکثر سطح 2 درصد پودر ماهی برای جیره آغازین جوجه‌های گوشتی مناسب است و سطوح بالاتر ممکن است مقدار ترشح داخلی پروتئین و اسید‌آمینه را افزایش و قابلیت هضم آنها را کاهش دهد.
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