Ileal Digestibility of Amino Acids in Fish Meal-Based Diets for Broiler Starters Using Regression Technique

محورهای موضوعی : Camel

آ.اف. آگبولا ¹ , ای.آ. لیایی ²

1 - Department of Animal Science, University of Ibadan, Ibadan, Nigeria
2 - Department of Animal Science, University of Ibadan, Ibadan, Nigeria

تاریخ دریافت : 1391/08/05 تاریخ پذیرش : 1391/10/28 تاریخ انتشار : 1392/09/10

کلید واژه: Amino acids, broiler starters, fish meal, ileal digestibility, regression technique,

چکیده مقاله :

An experiment was conducted to determine apparent ileal digestibility of crude protein (CP) and amino acids (AA) and digestible CP and AA of fish meal (FM) in 21-d-old broiler chickens using the regression technique. Four diets containing 0, 1, 2, and 3% FM in place of cornstarch were formulated with FM as the sole contributor of CP and AAs in the sequential diets. Eighty 14-d-old broiler chicks were weighed, and sorted by body weight and randomly allotted to the experimental diets with 4 replicates per diet and 5 chicks per replicate. Titanium dioxide was added to the diets at the rate of 5 g/kg diet as an indigestible marker. They were fed the experimental diets till d 21 post hatch when they were killed by asphyxiated with CO2 and digesta from the last two-third of ileum collected. The concentrations of CP and AA in the diets increased as dietary FM increased. Apparent digestibility of CP and AA significantly (P<0.05) increased with dietary FM inclusion but with a decrease in the 3% FM diet. The digestibility values of CP, lysine and methionine estimated as slopes of the regression of the amount of CP or AA digested dry matter intake dry matter intake (DMI) at the terminal ileum against CP or AA intake dry matter intake (DMI) were 88.7, 97.2 and 97.0% respectively. The digestible amounts of CP, lysine and methionine of FM were 63.63 g, 5361.04 mg and 1801.45 mg. Results of the study suggest a maximum level of 2% inclusion of FM was adequate for broiler starter chicks as higher levels may induce higher endogenous CP and AA flow and hence reduced digestibility of these nutrients.

چکیده انگلیسی:

برای اندازه‌گیری قابلیت ‌هضم ایلئومی ظاهری پروتئین‌خام و اسیدهای ‌آمینه و پروتئین و اسیدهای ‌آمینه قابل ‌هضم پودر ‌ماهی در جوجه‌های گوشتی 21 روزه با استفاده از روش تابعیت، آزمایشی انجام شد. چهار جیره که در آنها 0، 1، 2 و 3 درصد پودر ماهی جایگزین نشاسته ذرت شده بود فرموله شدند و پودر‌ ماهی به عنوان تنها تأمین کننده پروتئین و اسیدهای ‌آمینه در جیره‌ها بود. هشتاد قطعه جوجه گوشتی 14 روزه وزن شدند و براساس وزن بدن به طور تصادفی به جیره‌های آزمایشی با 4 تکرار و 5 جوجه در هر تکرار اختصاص یافتند. اکسید تیتانیوم به مقدار 5  g/kg به عنوان معرف غیرقابل ‌هضم اضافه شد. جوجه‌ها تا 21 روزگی از جیره‌ها تغذیه و سپس با استفاده از دی‌اکسید‌کربن خفه شدند. محتویات روده از دو سوم انتهایی ایلئوم جمع‌آوری گردید. با استفاده از پودر ‌ماهی، مقدار پروتئین و اسیدهای ‌آمینه جیره‌ها افزایش یافتند. گرچه با افزودن پودر ‌ماهی به جیره، به قابلیت ‌هضم ظاهری پروتئین و اسیدهای‌ آمینه اضافه شد (05/0>P) اما با 3 درصد پودر‌ ماهی کاهش یافت. قابلیت‌ هضم پروتئین، لیزین و متیونین به صورت شیب تابعیت پروتئین یا اسید‌ آمینه هضم شده در ماده ‌خشک مصرفی در انتهای ایلئوم در برابر پروتئین یا اسید ‌آمینه هضم ‌شده در ماده‌ خشک مصرفی به ترتیب 7/88، 2/97 و 97 درصد تخمین زده شد. مقدار قابل ‌هضم پروتئین، لیزین و متیونین پودر‌ ماهی 63/63، 04/536 و 45/1801 میلی‌گرم بود. نتایج تحقیق پیشنهاد می‌کند حداکثر سطح 2 درصد پودر‌ ماهی برای جیره آغازین جوجه‌های گوشتی مناسب است و سطوح بالاتر ممکن است مقدار ترشح داخلی پروتئین و اسید‌آمینه را افزایش و قابلیت هضم آنها را کاهش دهد.

منابع و مأخذ:

Adedokun S.A., Lilburn M.S., Parsons C.M., Adeola O. and Applegate T.J. (2007). Endogenous amino acid flow in broiler chicks is affected by the age of birds and method of estimation. Poult. Sci. 86, 2590-2597.
Adedokun S.A., Adeola O., Parsons C.M., Lilburn M.S. and Applegate T.J. (2008). Standardized ileal amino acid digestibility of plant feedstuffs in broiler chickens and turkey poults using a nitrogen-free or casein diet. Poult. Sci. 87, 2535-2548.
Adedokun S.A., Adeola O., Parsons C.M., Lilburn M.S. and Applegate T.J. (2011). Factors affecting endogenous amino acid flow in chickens and the need for consistency in methodology. Poult. Sci. 90, 1737-1748.
Agboola A.F. (2011). Precaecal digestible crude protein and amino acids in some conventional feedstuffs in broiler starter diets. Ph D. Thesis. Univ. Ibadan, Ibadan. Nigeria.
Angkanaporn K., Ravindran V. and Bryden W.L. (1997). Influence of caecetomy and dietary protein concentration on apparent excreta amino acid digestibility in adult cockerels. British Poult. Sci.38, 270-276.
AOAC. (2000). Official Methods of Analysis. 15^th Ed. Association of Official Analytical Chemists, Washington, DC.
Brandt M. and Allam S.M. (1987). Analytik von TiO₂ im Darminhalt und Kot nac KjeldahlaufschluB. Arch. Anim. Nutr. 37, 453-454.
Donkoh A. and Moughan P.J. (1999). Endogenous ileal nitrogen and amino acid flows in the growing pig receiving a protein-free diet and diets containing enzymically hydrolysed casein or graded levels of meat and bone meal. Anim. Sci. 68, 511-518.
Fan M.Z. and Sauer W.C. (1995). Determination of apparent ileal amino acid digestibility in barley and canola meal for pigs with the direct, difference, and regression methods. J. Anim. Sci. 73, 2364-2374.
Fan M.Z., Sauer W.C., Hardin R.T. and Lien K.A. (1994). Determination of apparent ileal amino acid digestibility in pigs: Effect of dietary AA level. J. Anim. Sci. 72, 2851-2859.
Fan M.Z., Archbold T., Sauer W.C., Lackeryam D., Rideout T. Gao Y., De Lange F.M. and Hacker R.R. (2001). Novel methodology allows simultaneous measurement of true phosphorus digestibility and the gastrointestinal endogenous phosphorus outputs in studies with pigs. J. Nutr. 131, 2388-2396.
Hoehler D., Lemme A., Ravindran V., Bryden W.L. and Rostagno H.S. (2006). Feed formulation in broiler chickens based on standardized ileal amino acid digestibility.Pp. 23-24 in Proc. 3^nd Mild-Atlantic Nutr.Conf., Timonium, Marylad.
Jansman A.J.W., Smink W., Van Leeuwen P. and Rademacher M. (2002). Evaluation through literature data of the amount of amino acid composition of basal endogenous crude protein at the terminal ileum of pigs. Anim. Feed Sci. Technol. 98, 49-60.
Kim E.J., Utterback P.L. and Parsons C.M. (2012). Comparison of amino acid digestibility coefficients for soybean meal, canola meal, fish meal, and meat and bone meal among 3 different bioassays. Poult. Sci. 91, 1350-1355.
Lemme A., Cremers S., Pallauf J., Rostagno H.S., Pack M. and Petri A. (2001). Apparent and true ileal amino acid digestibility of vegetable and animal protein of different origin in broilers. Pp. 169-170 in Proc. 13^th European Symp. Poult. Nutr. Blankenberge, Belgium.
Lemme A., Ravindran V. and Bryden W.L. (2004). Ileal digestibility of amino acids in feed ingredients for broilers. World’s Poult. Sci. J. 60, 423-437.
Ravindran V. and Blair R. (1993). Feed resources for poultry production in Asia and the pacific: III. Animal protein sources. World’s Poult. Sci. J. 47, 219-235.
Ravindran V. and Byden W.L. (1999). Amino acid availability in poultry in vitro and in vivo measurements.Aust. J. Agric. Res.50, 889-908.
Ravindran V. and Hendriks W.H. (2004). Endogenous amino acid flows at the terminal ileum of broilers, layers and adult roosters. Anim. Sci. 79, 265-271.
Ravindran V., Hew L.I. Ravindran G. and Bryden W.L. (2005). Apparent ileal digestibility of amino acids in dietary ingredients for broiler chickens. Anim. Sci. 81, 85-97.
Ravindran V., Hew L.L., Ravindran G. and Bryden W.L. (1999). A comparison of ileal digesta and excreta analysis for the determination of amino acid digestibility in food ingredients for poultry. British Poult. Sci. 40, 266-274.
Rezvani M., Kluth H. and Rodehutscord M. (2008). Comparison of amino acid digestibility determined prececally or based on total excretion of cecectomized laying hens. Poult. Sci. 87, 2311-2317.
Rodehutscord M., Kapocius M., Timmler R. and Dieckmann A. (2004). Linear regression approach to study amino acid digestibility in broiler chickens. British Poult. Sci. 45, 85-92.
SAS. (2006). SAS^®/STAT Software, Release 9.1 SAS Institute, Inc., Cary, NC.
Short F.J., Wiseman J. and Boorman K.N. (1999). Application of a method to determine ileal digestibility in broilers of amino acids in wheat. Anim. Feed Sci. Technol. 79, 195-209.