Association of Kappa-Casein Gene Polymorphism with some Biochemical Blood Indicators in Guilan Native Cattle of Iran (Bos indicus)

محورهای موضوعی : Camel

A. Sobar Poorrajabi Ghaziyani ¹ , S.Z. Mirhoseini ² , N. Ghavi Hossein-Zadeh ³ , Z. Ansari Pirsaraei ⁴ , H. Dehghanzadeh ⁵

1 - Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
2 - Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
3 - Department of Animal Science, Faculty of Agriculture Science, University of Guilan, Rasht, Iran
4 - Department of Animal Science, Faculty of Agriculture Science, University of Agriculture and Natural Resource, Sari, Iran
5 - Guilan Agriculture and Natural Resources Research Center, Rasht, Iran

تاریخ دریافت : 2014/02/25 تاریخ پذیرش : 2014/06/15 تاریخ انتشار : 2014/12/01

کلید واژه: PCR-RFLP, Cholesterol, glucose, Thyroxine, Triglycerides, Urea,

چکیده مقاله :

Evaluation of the blood and milk biochemical parameters always is considered as effective key factor for animal health and dairy products. In order to assess the association of kappa-casein (K-CN) genetic variants with blood biochemical indicators, blood samples were randomly and individually collected from 126 Iranian Guilan native cattle. Blood plasma was used to measure blood glucose, urea, cholesterol, triglycerides and thyroxine concentrations. The genomic DNA was extracted from the whole blood by the modified Salting Out method. The 350 bp fragment of kappa-casein gene was amplified using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and a pair of specific primers was digested by HinfI restriction enzyme. In general, two alleles of A and B with the frequencies of 0.726 and 0.274 and two genotypes of AA and AB with frequencies of 0.452 and 0.548 were detected, respectively. The chi-square test result showed that the studied population was not in Hardy-Weinberg equilibrium. Results of statistical analysis revealed that there was significant difference between glucose, cholesterol and thyroxin levels in both sexes which are associated with the greater levels of testosterone and thyroid hormones in bulls than cows. However, there was no significant difference between males and females for urea and triglyceride levels (P>0.05). There was no significant difference between AA and AB genotypes of kappa-casein (K-CN) gene in Guilan native cattle for the blood parameters measured (P>0.05). According to the current results, kappa casein gene singly cannot be an appropriate marker to study the blood parameters in native cattle of Guilan.

چکیده انگلیسی:

ارزیابی فراسنجه‌های بیوشیمیای خون و شیر همواره به عنوان شاخص‌هایی برای چگونگی سلامت حیوان و محصولات لبنی مرغوب مورد توجه بوده‌اند. برای بررسی ارتباط ژنوتیپ‌های مختلف ژن کاپا-کازئین با فراسنجه‌های خونی 126 رأس گاو بومی گیلان از هر دو جنس به طور تصادفی و انفرادی خونگیری به عمل آمد. با استفاده از روش بهینه شده نمکی DNA نمونه‌ها استخراج شدند.برای اندازه‌گیری سطوح گلوکز، اوره، کلسترول، تری‌گلیسرید و تیروکسین از پلاسمای خون استفاده شد. دو آلل A و B به ترتیب با فراوانی‌های 726/0، 274/0 و دو ژنوتیپ AA و AB به ترتیب با فراوانی 452/0، 548/0 در جمعیت مشاهد شد. احتمالاً به دلیل کم بودن تعداد نمونه‌ها و فراوانی کم آلل B، ژنوتیپ BB در جمعیت دیده نشد. جمعیت از نقطه نظر این جایگاه ژنی در تعادل هاردی- واینبرگ قرار نداشت. نتایج تجزیه و تحلیل‌های آماری نشان داد که بین سطوح گلوکز، کلسترول و تیروکسین در هر دو جنس اختلاف معنی‌دار وجود دارد که به میزان بسیار زیادتر غلظت هورمون تستوسترون، هورمون‌های تیروئیدی در جنس نر نسبت به جنس ماده مرتبط است، اما سطوح تری‌گلیسرید و اوره اختلاف معنی‌داری از خود نشان ندادند (05/0=α). ژنوتیپ‌های AA و AB ژن کاپا-کازئین در گاو بومی گیلان از نظر غلظت فراسنجه‌های خونی گلوکز، اوره، کلسترول، تری‌گلیسرید و تیروکسین تفاوت معنی‌داری با یکدیگر نداشتند (05/0=α). با توجه به مشاهدات و نتایج حاصل چند‌شکلی ژن کاپا-کازئین به تنهایی نمی‌تواند نشانگر مناسبی برای غلضت این قبیل فراسنجه‌های خونی بر روی گاو بومی گیلان باشد.

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