Evaluation of the Probiotic Potential of some Native Lactobacillus Strains on the Laying Performance and Egg Quality Parameters of Japanese Quails
محورهای موضوعی : Camelس.ع. سیادتی 1 , ی. ابراهیم نژاد 2 , غ. صالحی جوزانی 3 , ج. شایق 4
1 - Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran
2 - گروه علوم دامی دانشگاه آزاد اسلامی واحد شبستر
3 - Department of Microbial Biotechnology, Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4 - Department of Veterinary Medicine, Shabestar Branch, Islamic Azad University, Shabestar, Iran
کلید واژه: Probiotic, egg quality traits, laying quail, production parameters, native <i>Lactobacillus</i>,
چکیده مقاله :
The objective of the present study was to evaluate the probiotic effects of different concentrations of four selected native Lactobacillus strains on laying performance and egg quality parameters of quails. To do this, a six-week farm trial was performed in completely randomized design with seven different probiotic treatments of four replicates each consisting of eight quails, resulting in a total of 224 quails. Four native Lactobacillus strains whose probiotic potentials had previously been confirmed at in vitro levels were cultured in a 10 liter batch fermentor and lyophilized (1010 CFU/g). Treatments were as follows: T1: control (basal diet without probiotics); T2: basal diet + Primalac® (454 g/ton); T3: basal diet + Protexin® (50 g/ton); and T4, T5, T6, and T7: four native strains in levels of 50, 100, 150, and 200 g/ton diets, respectively. The native strains and Protexin® could significantly improve egg production, feed conversion ratio (FCR), and egg mass during the laying period (P<0.05), whereas no significant effect was observed on feed intake and egg weight. The probiotic treatments had significant positive effects on the albumen height, Haugh unit, and internal quality unit (IQU) (P<0.05), whereas there was no difference between the treatments in terms of egg shell weight, egg yolk weight, and egg albumen weight of the quails (P>0.05). It can be concluded that the use of native strains (a 50 g/ton diet) improved the performance (egg production, egg mass, and FCR) and egg quality parameters (Haugh unit and IQU) of quails.
هدف از این تحقیق ارزیابی تأثیر غلظت‌های مختلف چهار سویه بومی لاکتوباسیلوس بر عملکرد تخمگذاری و کیفیت تخم بلدرچین بود. برای انجام این تحقیق، شش هفته آزمایشات فارمی در قالب یک طرح کاملاً تصادفی با چهار تکرار، 7 تیمار و 8 قطعه بلدرچین به ازای هر تکرار بر روی 224 قطعه بلدرچین انجام شد. چهار سویه بومی لاکتوباسیلوس که قبلاً پتانسیل پروبیوتیکی آن‌ها در شرایط آزمایشگاهی ثابت شده بود در یک فرمانتور 10 لیتری کشت و سپس لیوفلیزه شدند (CFU/g 1010). تیمارها شامل: تیمار 1) شاهد (جیره پایه بدون پروبیوتیک)، تیمار 2) جیره پایه + پریمالاک (454 گرم/تن)، تیمار 3) جیره پایه + پروتکسین (50 گرم/تن) و تیمارهای 4، 5، 6 و 7 به ترتیب دارای 50، 100، 150 و 200 گرم از مخلوط چهار سویه بومی پروبیوتیک به ازای هر تن جیره غذایی بودند. سویه‌های بومی و پروبیوتیک تجاری پروتکسین توانستند به طور معنی‌داری درصد تولید، ضریب تبدیل غذایی و توده تخم را در طول دوره تخمگذاری بهبود ببخشند (P<0.05)، در حالیکه هیچ تأثیر معنی‌داری بر روی مصرف خوراک و وزن تخم مشاهده نشد (P>0.05). تیمارهای حاوی پروبیوتیک دارای تأثیر مثبت معنی‌داری بر روی ارتفاع سفیده تخم، واحد هاو و واحد کیفیت داخلی تخم بودند (P<0.05)، در حالیکه هیچ اختلاف معنی‌داری در بین تیمارها در خصوص صفات وزن پوسته، وزن زرده و وزن سفیده تخم بلدرچین‌ها مشاهده نشد (P>0.05). در انتها می‌توان نتیجه گرفت که استفاده از سویه‌های بومی لاکتوباسیلوس (50 گرم به ازای هر تن جیره) عملکرد تخمگذاری (درصد تولید تخم، توده تخم و ضریب تبدیل غذایی) و پارامترهای کیفی تخم بلدرچین (واحد هاو و واحد کیفیت داخلی) را بهبود بخشیدند.
Aazami N., Salehi Jouzani G., Khodaei Z., Meimandipour A., Safari M. and Goudarzvand M. (2014). Characterization of some potentially probiotic Lactobacillus strains isolated from Iranian native chickens. J. Gen. Appl. Microbiol. 60, 215-221.
Abdel-Azeem F., Nematallah G. and Ibrahim Faten A. (2005). Effect of dietary protein level with some natural biological feed additives supplementation on productive and physiological performance of Japanese quail. Egypt Poult. Sci. 25, 497-525.
Aghaii A., Chaji M., Mohammadabadi T. and Sari M. (2010). The effect of probiotic supplementation on production performance, egg quality and serum and egg chemical composition of laying hens. J. Anim. Vet. Adv. 9, 2774-2777.
Amerah A., Quiles A., Medel P., Sánchez J., Lehtinen M. and Gracia M. (2013). Effect of pelleting temperature and probiotic supplementation on growth performance and immune function of broilers fed maize/soy-based diets. Anim. Feed. Sci. Technol. 180, 55-63.
Ayasan T., Ozcan B.D., Baylan M. and Canogullari S. (2006). The effects of dietary inclusion of probiotic protexin on egg yield parameters of Japanese quails (Coturnix coturnix Japonica). Int. J. Poult. Sci. 5, 776-779.
Balevi T., Ucan U., Coşun B., Kurtoğu V. and Cetingül I. (2001). Effect of dietary probiotic on performance and humoral immune response in layer hens. British Poult. Sci. 42, 456-461.
Cean A., Stef L., Simiz E., Julean C., Dumitrescu G., Vasile A., Pet E., Drinceanu D. and Corcionivoschi N. (2015). Effect of human isolated probiotic bacteria on preventing Campylobacter jejuni colonization of poultry. Foodborne Pathog. Dis. 12, 122-130.
Chen C.Y., Tsen H.Y., Lin C.L., Yu B. and Chen C.S. (2012). Oral administration of a combination of select lactic acid bacteria strains to reduce the Salmonella invasion and inflammation of broiler chicks. Poult. Sci. 91, 2139-2147.
Chen Y., Nakthong C. and Chen T. (2005). Improvement of laying hen performance by dietary prebiotic chicory oligofructose and inulin. Int. J. Poult. Sci. 4, 103-108.
Choi S.B., Lew L.C., Yeo S.K., Nair Parvathy S. and Liong M.T. (2015). Probiotics and the BSH-related cholesterol lowering mechanism: a Jekyll and Hyde scenario. Crit. Rev. Biotechnol. 35, 392-401.
Christaki E., Bonos E. and Florou-Paneri P. (2011). Use of anise seed and/or α-tocopheryl acetate in laying Japanese quail diets. South African J. Anim. Sci. 41, 126-133.
Daneshmand A., Sadeghi G., Karimi A., Vaziry A. and Ibrahim S.A. (2015). Evaluating complementary effects of ethanol extract of propolis with the probiotic on growth performance, immune response and serum metabolites in male broiler chickens. Livest. Sci. 178, 195-201.
Davis G. and Anderson K. (2002). The effects of feeding the direct-fed microbial, primalac, on growth parameters and egg production in single comb white leghorn hens. Poult. Sci. 81, 755-759.
Edens F. (2003). An alternative for antibiotic use in poultry: probiotics. Brazilian J. Poult. Sci. 5, 75-97.
Gardiner G.E., Casey P.G., Casey G., Lynch P.B., Lawlor P.G., Hill C., Fitzgerald G.F., Stanton C. and Ross R.P. (2004). Relative ability of orally administered Lactobacillus murinus to predominate and persist in the porcine gastrointestinal tract. Appl. Environ. Microbiol. 70, 1895-1906.
Goodling A., Cerniglia G. and Hebert J. (1987). Production performance of white leghorn layers fed Lactobacillus fermentation products. Poult. Sci. 66, 480-486.
Guclu B.K. (2011). Effects of probiotic and prebiotic (mannan oligosaccharide) supplementation on performance, egg quality and hatchability in quail breeders. Vet. Fak. Derg. 58, 27-32.
Hippenstiel F., Abdel-Wareth A., Kehraus S. and Südekum K. (2011). Effects of selected herbs and essential oils, and their active components on feed intake and performance of broilers–a review. Arch. Geflugelkd. 75, 226-234.
Kalavathy R., Abdullah W., Jalaludin S., Wong C.M.V.L. and Ho Y. (2005). Effects of Lactobacillus cultures on performance and egg quality during the early laying period of hens. J. Anim. Feed. Sci. 14, 537-547, 2005.
Kalsum U., Soetanto H., Achmanu A. and Sjofjan O. (2012). Effect of a probiotic containing Lactobacillus salivarius on the laying performance and egg quality of Japanese quails. Livest. Res. Rural Dev. 24, 12-22.
Karimi-Kivi R., Dadashbeiki M. and Seidavi A. (2015). Growth, body characteristics and blood parameters of ostrich chickens receiving commercial probiotics. Spanish J. Agric. Res. 13(1), 0604.
Kaur S., Mandal A.B., Singh K.B., Kadam M.M. and Elangovan A.V. (2007). Response of laying Japanese quails to graded levels of essential amino acids profile with reduced dietary protein. J. Sci. Food Agric. 87, 751-759.
Kurtoglu V., Kurtoglu F., Seker E., Coskun B., Balevi T. and Polat E. (2004). Effect of probiotic supplementation on laying hen diets on yield performance and serum and egg yolk cholesterol. Food Addit. Contam. 21, 817-823.
Mahdavi A., Rahmani H. and Pourreza J. (2005). Effect of probiotic supplements on egg quality and laying hen's performance. Int. J. Poult. Sci. 4, 488-492.
Manafi M., Khalaji S. and Hedayati M. (2016). Assessment of a probiotic containing Bacillus subtilis on the performance and gut health of laying Japanese quails (Coturnix coturnix Japonica). Rev. Bras. Cienc. Avic. 18, 599-606.
Mátéová S., Gaálová M., Šály J. and Fialkovičová M. (2009). Investigation of the effect of probiotics and potentiated probiotics on productivity of laying hens. Czech J. Anim. Sci. 54, 24-30.
Mikulski D., Jankowski J., Naczmanski J., Mikulska M. and Demey V. (2012). Effects of dietary probiotic (Pediococcus acidilactici) supplementation on performance, nutrient digestibility, egg traits, egg yolk cholesterol, and fatty acid profile in laying hens. Poult. Sci. 91, 2691-2700.
Mohan B., Kadirvel R., Bhaskaran M. and Natarajan A. (1995). Effect of probiotic supplementation on serum/yolk cholesterol and on egg shell thickness in layers. British Poult. Sci. 36, 799-803.
Mookiah S., Sieo C.C., Ramasamy K., Abdullah N. and Ho Y.W. (2014). Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J. Sci. Food Agric. 94, 341-348.
Mountzouris K., Tsirtsikos P., Papadomichelakis G., Schatzmayr G. and Fegeros K. (2015). Evaluation of the efficacy of sequential or continuous administration of probiotics and phytogenics in broiler diets. Anim. Prod. Sci. 55, 720-728.
Nahashon S., Nakaue H. and Mirosh L. (1994). Production variables and nutrient retention in Single Comb White Leghorn laying pullets fed diets supplemented with direct-fed microbials. Poult. Sci. 73, 1699-1711.
NRC. (1994). Nutrient Requirements of Poultry, 9th Rev. Ed. National Academy Press, Washington, DC., USA.
Olgun O. and Yıldız A.Ö. (2014). Effects of diets Including different levels of protein and supplemented with probiotic-enzyme on performance and eggshell quality of laying quails. Turkish J. Agric. Food Sci. Technol. 2, 236-241.
Önol A.G., Sari M., Ogüz F.K., Gülcan B. and Erbas G. (2003). Sürekli sicak stresinde bulunan yumurtlama dönemindeki bildircinlarin rasyonlarina probiyotik katkisinin bazi verim ve kan parametreleri uzerine etkisi. Turkish J. Vet. Anim. Sci. 27, 1397-1402.
Panda A.K., Rama Rao S.S., Raju M.V. and Sharma S.S. (2008). Effect of probiotic (Lactobacillus sporogenes) feeding on egg production and quality, yolk cholesterol and humoral immune response of White Leghorn layer breeders. J. Sci. Food Agric. 88, 43-47.
Patterson J. and Burkholder K. (2003). Application of prebiotics and probiotics in poultry production. Poult. Sci. 82, 627-631.
Pournazari M., Qotbi A.A., Seidavi A. and Corazzin M. (2017). Prebiotics, probiotics and thyme (Thymus vulgaris) for broilers: Performance, carcass traits and blood variables. Rev. Colomb. Cienc. Pec. 30(1), 3-10.
Ramasamy K., Abdullah N., Jalaludin S., Wong M. and Ho Y.W. (2009). Effects of Lactobacillus cultures on performance of laying hens, and total cholesterol, lipid and fatty acid composition of egg yolk. J. Sci. Food. Agric. 89, 482-486.
Ramirez-Chavarin M., Wacher C., Eslava-Campos C. and Perez-Chabela M. (2013). Probiotic potential of thermotolerant lactic acid bacteria strains isolated from cooked meat products. Int. Food Res. J. 20, 991-1000.
Roberts J. R. (2004). Factors affecting egg internal quality and egg shell quality in laying hens. J. Poult. Sci. 41, 161-177.
Rodriguez L., Haro J., Barrera E., Oporta M., Alcorta M. and Bojalil C. (2002). Citric acid and microbial phytase relative to productive performance and phosphorus, calcium and nitrogen excretion in laying hens. Téc. Pecu. Méx. 40, 169-180.
SAS Institute. (2003). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Smug L., Salminen S., Sanders M. and Ebner S. (2014). Yoghurt and probiotic bacteria in dietary guidelines of the member states of the European Union. Benef. Microbes. 5, 61-66.
Tellez G., Pixley C., Wolfenden R., Layton S. and Hargis B. (2012). Probiotics/direct fed microbials for Salmonella control in poultry. Food Res. Int. 45, 628-633.
Timmerman H., Koning C., Mulder L., Rombouts F. and Beynen A. (2004). Monostrain, multistrain and multispecies probiotics—A comparison of functionality and efficacy. Int. J. Food Microbiol. 96, 219-233.
Tortuero F. and Fernandez E. (1995). Effects of inclusion of microbial cultures in barley-based diets fed to laying hens. Anim. Feed Sci. Technol. 53, 255-265.
Tůmová E. and Gous R. (2012). Interaction of hen production type, age, and temperature on laying pattern and egg quality. Poult. Sci. 91, 1269-1275.
Vandeplas S., Dauphin R.D., Thiry C., Beckers Y., Welling G.W., Thonart P. and Thewis A. (2009). Efficiency of a Lactobacillus plantarum-xylanase combination on growth performances, microflora populations, and nutrient digestibilities of broilers infected with Salmonella typhimurium. Poult. Sci. 88, 1643-1654.
Vatsalya V. and Arora K.L. (2011). Association between body weight growth and selected physiological parameters in male Japanese quail (Coturnrix coturnix Japonica). Int. J. Poult. Sci. 10, 680-684.
Wang J., Zhang H., Chen X., Chen Y. and Bao Q. (2012). Selection of potential probiotic lactobacilli for cholesterol-lowering properties and their effect on cholesterol metabolism in rats fed a high-lipid diet. J. Dairy Sci. 95, 1645-1654.
Wu X.Y. (2006). Studies on the impact of probiotic bacteria on enteric microbial diversity and immune response. Ph D. Thesis. University of Wollongong, Wollongong, Australia.
Xu C.L., Ji C., Ma Q., Hao K., Jin Z.Y. and Li K. (2006). Effects of a dried Bacillus subtilis culture on egg quality. Poult. Sci. 85, 364-368.
Yalçin S., Özsoy B. and Erol H. (2008). Yeast culture supplementation to laying hen diets containing soybean meal or sunflower seed meal and its effect on performance, egg quality traits, and blood chemistry. J. Appl. Poult. Res. 17, 229-236.
Yörük M., Gül M., Hayirli A. and Macit M. (2004). The effects of supplementation of humate and probiotic on egg production and quality parameters during the late laying period in hens. Poult. Sci. 83, 84-88.
Zarei M., Ehsani M. and Torki M. (2011). Dietary inclusion of probiotics, prebiotics and synbiotic and evaluating performance of laying hens. Am. J. Agric. Bio. Sci. 6, 249-255.
Zhang J., Xie Q., Ji J., Yang W., Wu Y., Li C., Ma J. and Bi Y. (2012). Different combinations of probiotics improve the production performance, egg quality, and immune response of layer hens. Poult. Sci. 91, 2755-2760.
