Assessment of Biochemical and Histopathological Changes in the Liver of Chick Embryo Treated with a Commercial Deltamethrin Formulation
محورهای موضوعی : Camel
1 - Department of Zoology, IIS University, Jaipur, Rajasthan, India
2 - Department of Zoology, IIS University, Jaipur, Rajasthan, India
کلید واژه: biochemical, chick embryo, deltamethrin, hepatotoxic, pathological, synthetic pyre‐throid,
چکیده مقاله :
We studied the hepatotoxic effects of a deltamethrin containing formulation (Decis®) on developing em-bryo of Gallus domesticus. Fertilized eggs were immersed in three different concentrations of deltamethrin (12.5, 25 and 50 mg/L) for 60 min at 37 ˚C prior to incubation (ED 0) and on the 4th day of incubation (ED 4) and incubated till embryonic day (ED) 16. Exposure to 50 mg/L of deltamethrin resulted in a significant decrease in total protein and total glycogen content in the liver when exposed on ED 4 and ED 0 respec-tively, while total glutathione content was reduced at concentrations applied on both the exposure days. Among enzymes, liver alkaline phosphatase activity was significantly increased at 25 and 50 mg/L deltame-thrin when embryos were exposed on ED 0 and 4, whereas the glutamate pyruvate transaminase activity showed a marked increase in animals exposed to 50 mg/L deltamethrin only at ED 4. Histophathological evaluation revealed mild to marked cellular lesions in liver sections of insecticide treated embryo. The magnitude of most of these changes appeared to be dose dependent being more severe on exposure to high-est dose of deltamethrin. The observed pathological changes include degeneration and necrosis of hepato-cytes, cytoplasmic vacuolization, enlarged and congested sinusoidal spaces, leucocyte infiltrations and con-gestion and dilation of the central vein.
در این مطالعه اثرات هپاتوتوکسیک دلتامترین با فرمولاسیون Decis® بر تکامل جنین Gallus domesticus بررسی شده است. تخمهای منجمد شده به مدت 60 دقیقه در دمای 37 درجه سلسیوس در سه غلظت مختلف دلتامترین (5/12، 25 و 50 میلیگرم در لیتر) در زمانهای پیش از انکوباسیون (0=ED) و روز چهارم انکوباسیون (4=ED) غوطهور شده و تا روز جنینی (16=ED) انکوباسیون گردیدند. تیمار 50 میلیگرم دلتامترین در 0 =ED و 4 =ED به ترتیب کاهش معنی داری در محتوای پروتئین کل و گلیکوژن کل کبد نشان دادند. ولی محتوای گلوتاتیون در هر دو ED در همه غلظتها کاهش یافت. در بین آنزیمها، فعالیت آلکالین فسفاتاز کبدی به طور معنی داری در 25 و 50 میلیگرم در لیتر دلتامترین در زمانهای 0 =ED و 4 =ED به طور معنی داری افزایش یافته ولی فعالیت گلوتامات پیرووات ترانس آمیناز تنها در غلظت 50 میلیگرم در لیتر دلتامترین و و 4 =ED افزایش معنی داری نشان داد. در ارزیابی هیستوپاتولوژیکی، ضایعات سلولی متوسط تا زیاد در بخش‌هایی از کبد جنینهای تیمار شده با حشرهکش دیده شد. این نتایج نشان داد با افزایش بیشتر غلظت دلتامترین مورد استفاده، میزان اغلب این تغییرات نیز شدیدتر بود. تغییرات پاتولوژیکی مشاهده شده شامل دژنره شدن و نکروز سلولهای کبدی، واکوئله شدن سیتوپلاسمی، توسعه و پرخونی فضاهای سینوزوئید عدم فیلتراسیون لوکوسیتها و گشادی و پرخونی سیاهرگ میانی بودند.
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