Genetic Variation of Cytochrome b Gene in Three Local Goat Breeds in Central Java of Indonesia
محورهای موضوعی : Camelس. سوتوپو 1 , ا. پوربوواتی 2 , ا. کورنیانتو 3
1 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
2 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
3 - Department of Animal Production, Faculty of Animal and Agricultural Science, Diponegoro University, Tembalang Campus, Semarang, Indonesia
کلید واژه: genetic diversity, mitochondrial DNA, indigenous goat,
چکیده مقاله :
This study was conducted to elucidate the genetic diversity and phylogenic relationship of three local goat breeds in Central Java of Indonesia based on the cytochrome b (cyt b) gene sequence. Blood samples of 19, 15 and 12 heads of Kacang, Kejobong and Ettawa Grade breeds, respectively, were used as materials of the study. The multiple alignment of cyt b gene was conducted by comparing under study goat breeds and one deposited sequence in GeneBank (Capra hircus, accession number AB004072). Of the 16 sites of different nucleotide bases found, there were 12 sites that could be used as a marker to distinguish the three breeds of local goat in Indonesia with the Capra hircus. Genetic distance among local goats was quite close compared to genetic distance between those local goats to GenBank’s Capra hircus. Phylogeny tree showed two main clusters, that was cluster A for Capra hircus and cluster B for three local goat breeds of Indonesia.
این پژوهش برای توضیح تنوع ژنتیکی و ارتباط فیلوژنیکی سه نژاد بز بومی در جاوه مرکزی اندونزی بر اساس توالی ژن سیتوکروم b (cyt b) انجام شد. نمونه‌های خون به ترتیب از 19، 15 و 12 رأس از نژادهای Kacang، Kejobong وEttawa Grade، به عنوان مواد پژوهش استفاده شدند. برهم‌گذاری چندگانه ژن cyt b توسط مقایسه نژادهای بز تحت مطالعه و یک توالی ارائه شده در بانک ژن (کاپرا هیرکوس، شماره دسترسی AB004072) انجام شد. از 16 جایگاه متفاوت جفت بازی یافت شده، 12 جایگاه وجود دارد که می‌تواند به عنوان نشانگر برای تشخیص سه نژاد بز بومی در اندونزی با کاپرا هیرکوس استفاده شوند. فاصله ژنتیکی درمیان بزهای بومی در مقایسه با فاصله ژنتیکی بین بزهای بومی با توالی ژن بانک کاپرا هیرکوس کاملاً نزدیک بود. درخت فیلوژنی دو خوشه اصلی را نشان داد، که خوشه A برای کاپرا هیرکوس و خوشه B برای سه نژاد بز بومی اندونزی بود.
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