Polymorphism of some Genes Associated with Meat-Related Traits in Egyptian Sheep Breeds
محورهای موضوعی : Camelکا.ف. ماهروس 1 , م.اس. حسنان 2 , م. ابدل مردی 3 , اچ.آی. شافی 4 , اچ.ای. روشدی 5
1 - Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
2 - Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
3 - Department of Zoology, Faculty of Science, Ain Shams University, Cairo, Egypt
4 - Department of Cell Biology, National Research Center, Dokki, Giza, Egypt
5 - Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt
کلید واژه: PCR-RFLP, Sheep, genetic polymorphism,
چکیده مقاله :
The genetic polymorphism of some genes related to meat production in three Egyptian sheep breeds (Barki, Rahmani and Osseimi) was studied. The candidate genes were: Calpastatin, Myostatin, Diacylglycerol-acyltransferase1, Insulin-like growth factor binding protein-3 and Booroola fecundity gene. The technique applied was the restriction fragment length polymorphism for the polymerase chain reaction products. Polymorphism was found in the genes: Calpastatin, MyostatinandDiacylglycerol-acyltransferase 1, while no polymorphism was exhibited by the other two genes, Insulin-like growth factor binding protein-3 and the Booroola fecundity in the three breeds understudy. Calpastatin locus digested with MspI had two genotypes MM and MN. The highest allelic frequency was for allele M. The same locus Calpastatin digested with NcoI also exhibited two genotypes MM and MN. The NN genotype was absent with both the MspI and the NcoI enzymes in all breeds. Myostatin digested with DraI had two genotypes AB and BB. The AA genotype cannot be detected. The highest allelic frequency was for allele B. Diacylglycerol-acyltransferase1 digested with AluI showed two genotypes CC and CT. The highest allelic frequency was for allele C. The detected CT genotype might explain the moderate intramuscular fat content and muscle marbling in the Egyption sheep breeds. Each of the remaining two loci (Insulin-like growth factor binding protein-3 and the Booroola fecundity) had only one genotype, BB genotype for Insulin-like growth factor binding protein-3 digested with HaeIII and ++ genotype for the Booroola fecundity digested with AvaII enzyme, therefore they are not recommended in the selection program. The result of Chi-square analysis indicated that the three Egyptian sheep breeds were in Hardy-Weinberg equilibrium.
چندشکلی ژنتیکی برخی از ژنهای مرتبط با تولید گوشت در سه نژاد گوسفند مصری (بارکی، رحمانی و اُسیمی) مورد مطالعه قرار گرفت. ژنهای کاندیدا شامل کالپاستاتین، میوستاتین، دیآسیل گلیسرول استیل ترانسفراز 1، پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی و ژن باروری برولا بودند. برای این هدف از روش PCR-RFLP استفاده شد. چندشکلی در ژنهای کالپاستاتین، میوستاتین و دیآسیل گلیسرول استیل ترانسفراز 1 مشاهده گردید ولی در ژنهای پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی و ژن باروری برولا چندشکلی دیده نشد. کالپاستاتین با کمک آنزیم MspI هضم شده و دو ژنوتیپ MM و MN مشاهده شد. بیشترین فراوانی آللی مربوط به آلل M بود. جایگاه کالپاستاتین با کمک آنزیم NcoI نیز هضم شده و ژنوتیپهای MM و MN دیده شدند. ژنوتیپ NN در هر دو آنزیم MspI و NcoI در کلیه نژادها دیده نشد. میوستاتین توسط DraI هضم شده و دو ژنوتیپ AB و BB مشاهده گردید. ژنوتیپ AA تشخیص داده نشد. بیشترین فراوانی آللی مربوط به آلل B بود. دیآسیل گلیسرول استیل ترانسفراز 1 توسط AluI هضم شد و دو ژنوتیپ CC و CT ایجاد نمود. بیشترین فراوانی آللی مربوط به آلل C بود. ژنوتیپ CT میتواند بیانگر وجود چربی بین ماهیچهای و ماربلینگ ماهیچهای متوسط در نژادهای گوسفند مصری باشد. هر کدام از دو جایگاه ژنی دیگر (پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی و ژن باروری برولا) تنها یک ژنوتیپ (ژنوتیپ BB در پروتئین شماره 3 متصل شوده به فاکتور رشد شبه انسولینی هضم شده با آنزیم HaeIII و ژنوتیپ ++ در ژن باروری برولا هضم شده با آنزیم AvaII) داشتند. بنابراین استفاده از این جایگاهها در برنامههای انتخاب توصیه نمیشود. نتایج آنالیز مربع کای نیز نشان داد که این سه نژاد گوسفند مصری در تعادل هاردی-واینبرگ بودند.
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