Relationship of Dietary Fat Sources with Semen Characteristics, Blood Plasma Metabolites and Scrotal Circumference in Mature Rams
محورهای موضوعی : Camelآ. رادمنش 1 , ح. درمانی کوهی 2 , آ. ریاسی 3
1 - Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
2 - Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Isfehan, Isfehan, Iran
کلید واژه: Fertility, dietary fat source, ram, full fat soybean, spermatozoid,
چکیده مقاله :
The hypothesis of this experiment was that the various sources of dietary fat with different dietary ratios of polyunsaturated fatty acids (PUFAs) to saturated fatty acids (SFAs) would alter the reproduction parameters of mature rams. Twelve mature rams were randomly allotted to one of four dietary treatments in a completely randomized design. Dietary treatments were: 1) ration with 4% soybean oil (SOY-OIL), 2) ration with 8% full fat soybean (FULL-FAT), 3) ration with 4% calcium salts of soybean oil (Ca-SALT) and 4) ration with 4% tallow (TALL). All diets were kept isoenergetic and isonitrogenous and formulated to be similar in Ca and P contents, while meeting or exceeding the Cornell Net Carbohydrate and Protein System software requirements for the rams used in this study. Diets were supplied to the rams during four months (from August to late December). Semen characteristics, scrotal circumference, and concentrations of cholesterol, low-density lipoprotein(LDL), high-density lipoprotein(HDL), triglyceride and testosterone of plasma were measured at the end of the experimental period. Based on the results of this study, diets supplemented with Ca-SALT and TALL improved the health of plasma membrane, viability and sperm concentration (P<0.05). Rams supplemented with Ca-SALT had also greater volume of semen and total sperm count in ejaculate (P<0.05) which are two important factors determining the sperm quality. There was no beneficial effect for different treatments based on spermatozoa motility (P>0.05). Blood concentrations of cholesterol, LDL and HDL of rams supplemented with Ca-SALT were significantly higher than the other treatments. Concentrations of triglyceride, testosterone and scrotal circumference were not affected by the experimental treatments (P>0.05). As an overall conclusion, the results of this study have indicated the benefits for adding Ca-SALT to the diets of adult rams on their reproductive performance.
فرض این آزمایش این بود که منابع گوناگون چربی جیرهای حاوی نسبتهای متفاوت اسیدهای چرب غیر اشباع با چند باند دوگانه به اسیدهای چرب اشباع، پارامترهای تولید‌مثلی قوچهای بالغ را تغییر خواهد داد. دوازده قوچ بالغ به طور تصادفی به یکی از چهار تیمار جیرهای در یک طرح کاملاً تصادفی اختصاص داده شدند. تیمارهای جیرهای عبارت بودند از: 1) جیره با 4 درصد روغن سویا (SOY-OIL)، 2) جیره با 8 درصد دانه سویای کامل (FULL-FAT)، 3) جیره با 4 درصد نمکهای کلسیمی روغن سویا (Ca-SALT)، و 4) جیره با 4 درصد پیه (TALL). تمامی جیرهها از نظر محتوای انرژی، پروتئین، کلسیم و فسفر یکسان بوده و طوری فرموله شده بودند که نیازمندیهای قوچهای بالغ را بر اساس سیستم کربوهیدرات و پروتئین خالص کرنل برآورده نمایند. جیرهها در طی چهار ماه (اول مرداد تا آخر آبان) در اختیار قوچها قرار گرفت. خصوصیات منی، محیط کیسه بیضه و غلظتهای کلسترول، لیپوپروتئینهای با چگالی پایین (LDL)، لیپوپروتئینهای با چگالی بالا (HDL)، تریگلیسرید و تستوسترون پلاسمای در انتهای دوره آزمایش اندازهگیری شدند. براساس نتایج آزمایش حاضر، جیرههای مکمل شده با Ca-SALT و TALL، سلامت غشای اسپرم، زنده مانی و غلظت اسپرم در هر انزال را بهبود داد (05/0>P). قوچهای مکمل شده با Ca-SALT حجم بیشتری از منی و شمار زیادتری از اسپرم، که جزء فاکتورهای تعیین کننده کیفیت اسپرم هستند، را دارا بودند (05/0>P). تفاوت معنی داری بین تیمارهای مختلف بر اساس قابلیت تحرک اسپرم مشاهده نشد. غلظتهای خونی کلسترول، LDL، HDL در قوچهای مکمل شده با Ca-SALT در مقایسه با سایر تیمارها بالاتر بود (05/0>P). غلظتهای تریگلیسرید، تستوسترون و محیط کیسه بیضه توسط تیمارهای آزمایشی تأثیر نپذیرفت. به عنوان نتیجهگیری کلی، نتایج این آزمایش سودمندی اضافه نمودن نمکهای کلسیمی روغن سویا را روی عملکرد تولید‌مثلی قوچهای بالغ نشان داد.
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