Effect of Synbiotic on Performance, Intestinal Morphology, Fecal Microbial Population and Blood Metabolites of Suckling Lambs
محورهای موضوعی : Camelا. معرب 1 , ت. قورچی 2 , س. رمضانپور 3 , ف. گنجی 4 , ع.ر. کوچک زاده 5
1 - Department of Animal Science, University of Agricultural Science and Natural Resources of Gorgan, Gorgan, Iran
2 - Department of Animal Science, University of Agricultural Science and Natural Resources of Gorgan, Gorgan, Iran
3 - Department of Animal Science, University of Agricultural Science and Natural Resources of Gorgan, Gorgan, Iran
4 - Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran
5 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
کلید واژه: Performance, Synbiotic, fecal, intestinal microflora, suckling lamb,
چکیده مقاله :
The effects of synbiotic supplementation to the Moghani suckling lambs diet were investigated using 18 lambs (initial BW 5-6 kg, age 3±2 day) were divided into 3 groups and fed experimental diets for 90 days. The dietary treatments were: 1) control (lambs were fed with mother’s milk, along with their normal diets), 2) control + 3 g of synbiotic and control and 3) control + 6 g of synbiotic for each lamb per day. Feed intake (FI), body weight gain (BWG) and feed conversion ratio (FCR) were measured monthly throughout the experimental period. Blood samples were taken at the beginning and end of the experiment. Intestinal microflora was assessed monthly in feces, assaying for coliform bacteria, total aerobics and lactic acid bacteria. Also, at the end of study 4 lambs were slaughtered for assaying intestinal morphology changes. The lambs that fed 3 g synbiotic had a higher BW, greater BWG, increased FI and a better FCR compared to the control diet although the differences not statistically significant. Supplementation with 3 g symbiotic significantly reduced (P<0.05) serum cholesterol levels. Fecal coliform bacteria were reduced and lactic acid bacteria increased by supplementation. The results indicate that synbiotic can be used as a growth promoter in suckling lamb’s diets and can improve their gut health.
تأثیر افزودن مکمل سینبیوتیک در جیره برههای شیرخوار نژاد مغانی مورد آزمایش قرار گرفت. هیجده بره به سه گروه (6 رأس در هر گروه براساس وزن 5-6 ماه و سن 2±3 روز) تقسیم شد و دوره پرورش 90 روز بود. تیمارها عبارت بودند از: شاهد (برههای تغذیه شده با شیر مادر همراه با جیره آغازین)، جیره شاهد + 3 گرم سینبیوتیک به ازای هر بره در روز، و جیره شاهد + 6 گرم سینبیوتیک به ازای هر بره در روز. مصرف ماده خشک، افزایش وزن روزانه و ضریب تبدیل خوراک به طور ماهانه در طول دوره پرورش اندازهگیری شد. نمونهگیری خون در روزهای ابتدا و انتهای دوره پرورش انجام گرفت. نمونهگیری مدفوع برای اندازهگیری بار میکروبی مدفوع هر ماه در طی پرورش انجام شد و کلیفرمها، لاکتو باسیلوسها و باکتریهای هوازی کل اندازهگیری شدند. در پایان دوره پرورش تعداد 4 بره از هر گروه به منظور بررسی تغییرات مورفولوژیکی روده کشتار شدند. تیمار حاوی 3 گرم سینبیوتیک به ازای هر بره در روز بیشترین مقدار افزایش وزن روزانه، وزن بدن، مصرف خوراک روزانه و بهترین ضریب تبدیل را نسبت به گروه شاهد داشت، اما تفاوت معنیداری مشاهده نشد. استفاده 3 گرم سینبیوتیک به طور معنیداری کلسترول خون را کاهش داد. تعداد کلیفرمهای مدفوع و لاکتو باسیلها به ترتیب به طور معنیداری کاهش و افزایش پیدا کرد. نتایج نشان داد سینبیوتیک میتواند به عنوان محرک رشد در برهها استفاده شود و باعث بهبود سلامتی روده آنها شود.
Antunovic Z., Speranda M., Amidzic D., Seric V., Steiner Z., Doma-Cinovic N. and Boli F. (2006). Probiotic application in lambs nutrition. Krmiva. 4, 175-180.
Awad W.A., Ghareeb K., Abdel-Raheem S. and Böhm J. (2009). Effects of dietary inclusion ofprobiotic and synbiotic on growth performance, organ weights and intestinal histomorphology of broiler chickens. Poult. Sci. 88, 49-55.
Bruce B.B., Gilliland S.E., Bush L.J. and Staley T.E. (1979). Influence of feeding cells of Lactobacillus acidophilus on the fecal flora of young dairy calves. Okla. Agric. Exp. Stn. 104, 207-209.
Caspary W.F. (1992). Physiology and pathophysiology of intestinal absorption. Am. J. Clin. Nutr. 55, 299-308.
Collins M.D. and Gibson G.R. (2012). Probiotics, prebiotics and synbiotics: approaches for modulating the microbial ecology of the gut. Am. J. Clin. Nutr. 69, 1052-1057.
Dayani O., Dadvar P. and Afsharmanesh M. (2011). Effect of dietary whole cottonseed and crude protein level on bloodparameters and performance of fattening lambs. Small Rumin. Res. 97, 48-54.
Deng Z.Y., Zhang J.W., Li J., Fan Y.W., Cao S.W., Huang R.L., Yin Y.L., Zhong H.Y. and Li T.J. (2007). Effect of polysaccharides of Cassiae seeds on the intestinal microflora of piglets. Asia Pacific J. Clin. Nutr. 16(1), 143-147.
De Valdez D.F., Martos G., Taranto M.P., Lorca G.L., Oliver G. and De Ruiz Holgado A.P. (1997). Influence of bile on b-galactosidase activity and cell viability of Lactobacillus reuteri when subjected to freeze-drying. J. Dairy Sci. 80, 1955-1958.
Dimitroglou A., Merrifield D.L., Moate R., Davies S.J., Spring P., Sweetman J. and Bradley G. (2009). Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout, Oncorhynchus mykiss (Walbaum). J. Anim. Sci. 87, 3226-3234.
Ellinger D.K., Muller L.D. and Glantz P.J. (1980). Influence offeeding fermented colostrum and Lactobacillus acidophiluson fecal flora of dairy calves. J. Dairy Sci. 63, 478-482.
Ewaschuk J.B., Naylor J.M., Chirino-Trejo M. and Zello G.A. (2004). Lactobacillus rhamnosus strain GG is a potential probiotic for calves. Canadian J. Vet. Res. 68, 249-253.
Fleige S., Preibinger W., Meyer H.H.D. and Pfaffl M.W. (2009). The immunomodulatory effect of lactulose on Enterococcus faecium fed preruminant calves. J. Anim. Sci. 87, 1731-1738.
Frizzo L.S., Sotto L.P., Zbrun M.V., Bertozzi E., Sequeira G., Armesto R.R. and Rosmini M.R. (2010). Lactic acid bacteria to improve growth performance in young calves fed milk replacer and spray-dried whey powder. Anim. Feed Sci. Technol. 157, 159-167.
Fuller R. (1977). The importance of Lactobacilli in maintaining normal microbial balance in the crop. Br. Poult. Sci. 18, 85-94.
Gibson G.R. and Roberfroid M.B. (1995). Dietary modulation of the human colonic microbiota. Introducing the concept of prebiotics. J. Nutr. 125, 1401-1412.
Gibson G.R. and Fuller R. (2000). Aspects of in vitro and in vivo research approaches directed toward identifying probiotics and prebiotics for human use. J. Nutr. 130, 391-395.
Green A.A. and Sainsbury D.W.B. (2001). The role of probiotic in producing quality poultry products. Pp. 245-251 in Proc. 15th European Symp. Qual. Poult. Meat. Kusadasi, Turkey.
Heinrichs A.J., Jones C.M., Elizondo-Salazar J.A. and Terrill S.J. (2009). Effects of aprebiotic supplement on health of neonatal dairy calves. Livest. Sci. 125, 149-154.
Hillal H., El-Sayaad G. and Abdella M. (2011). Effect of growth promoters (probiotics) supplementation on performance, rumen activity and some blood constituents in growing lambs. Inst. Farm. Anim. Biol. 6, 607-617.
Huang M.K., Choi Y.J., Houde R.J., Lee W., Lee B.H. and Zhao X. (2004). Effects of Lactobacilli and an Acidophilic fungus on the production performance and immune responses in broiler chickens. Poult. Sci. 83, 788-795.
Iji P.A., Saki A.A. and Tivey D.R. (2001). Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Anim. Feed Sci. Technol. 89, 175-188.
Isolauri E., Sutas Y., Kankaanpaa P., Arvilommi H. and Salminen S. (2001). Probiotics: effects on immunity. Am. J. Clin. Nutr. 73, 444-450.
Keithly J.I., Kott R.W., Berardinelli J.G., Moreaux S. and Hatfield P.G. (2011). Thermogenesis, blood metabolites and hormones, and growth of lambs born to ewes supplemented with algae-derived docosahexaenoic acid. J. Anim. Sci. 89, 4305-4313.
Kong X.F., Wu G.Y., Yin Y.L., Liu H.J., Yin F.G., Li T.J., Huang R.L., Kang P., Xing F., Fan M.Z., Yang C.B. and He Q.H. (2007). Dietary supplementation with Chinese herbal ultra-fine 3 powder enhances cellular and humoral immunity in early weaned piglets. Livest. Sci. 108, 94-98.
Krehbiel C.R., Rust S.R., Zhang G. and Gilliland S.E. (2003). Bacterial direct-fed microbials in ruminant diets: performance response and mode of action. J. Anim. Sci. 81, 120-132.
Langford R.M., Weary D.M. and Fisher L. (2003). Antibiotic resistance in gut bacteria from dairy calves: a dose response to the level of antibiotics fed in milk. J. Dairy Sci. 86, 3963-3966.
Lather A.L. (1975). In Cantarow and Trumper, Clinical Biochemistry. Souders, Co., Toranto.
Lema M., Williams L. and Rao D.R. (2001). Reduction of fecal shedding of enterohemorrhagic Escherichia coli O157:7 in lambs by feeding microbial feed supplement. Small Rumin. Res. 31, 31-39.
Mann S.O., Grant C. and Hobson P.N. (1980). Interactions of E. Coli and lactobacilli in gnotobiotic lambs. Microb. Let. 15, 141-144.
Moore J. (2004). The use of probiotics in the calf: an overview. Cattl Pract. 12, 125-128.
NRC. (1985). Nutrient Requirements of Sheep. 6thEd.NationalAcademy Press, Washington, DC, USA.
Patterson J.A. and Burkholder K.M. (2003). Application of prebiotics and probiotics in poultry production. Poult. Sci. 82, 627-631.
Potten C.S. (1977). Extreme sensitivity of some intestinal crypt cells to X and Y irradiation. Nature. London. 269, 518-521.
Sadik M.F. (1989). Effect of Lactobacillus concentrate (LBC) as a new growth promoter on the performance of growing buffalo heifers raised on milk replacer. MS Thesis. AinShams Univ., Cairo, Egypt.
Salama A.A.K., Caja G., Garin D., Albanell E., Such X. and Casals R. (2002). Effects of adding a mixture of malate and yeasts culture (Saccharomyces cerevisiae) on milk production of Murciano-Granadina dairy goats. Anim. Res. 51, 295-303.
Salem F.A., El-Amary H. and Hassanin S.H. (2001). Effect of bentonite supplementation on nutrients digestibility, rumen fermentation, some blood physiological parameters and perfromance of growing lambs. Egyptian J. Nutr. Feeds. 4, 179-191.
SAS Institute. (2001). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Siggers R.H., Thymann T., Siggers J.L., Schmidt M., Hansen A.K. and Sangilda P.T. (2007). Bacterial colonization affects early organ and gastrointestinal growth in theneonate. Livest. Sci. 109, 14-18.
Stella A.V., Paratte R., Valnegri L., Cigalino G., Soncini G., Chevaux E., Dell'Orto V. and Savoini G. (2007). Effect of administration of live Saccharomyces cerevisiae on milk production, milk composition, blood metabolites and faecal flora in early lactatingdairy goats. Small Rumin. Res. 67, 7-13.
Tripathi M.K. and Karim S.A. (2011). Effect of yeast cultures supplementation on live weight change, rumen fermentation, ciliate protozoa population, microbial hydrolytic enzymes status and slaughtering performance of growing lamb. Livest. Sci. 135, 17-25.
Tripathi M.K., Karim S.A., Chaturvedi O.H. and Verma D.L. (2007). Effect of different liquid cultures of live yeast strains on performance, ruminal fermentation and microbial protein synthesis in lambs. J. Anim. Physiol. Anim. Nutr. 92, 631-639.
Walter J., Tannkock G.W., Tilsala-Timsjarvi A., Rodtong S., Loach D.M., Munro K. and Alatossava A. (2000). Detection and identification of gastrointestinal lactobacillus species by using denaturing gradient gel electrophoresis and species-specific PCR primers. Appl. Environ. Microbiol. 66, 297-303.
Willard M.D., Simpson R.B., Cohen N.D. and Clancy J.S. (2000). Effects of dietary fructo-oligosaccharide on selected bacterial populations in feces of dogs. Am. J. Vet. Res. 61, 820-825.
Xu Z.R., Hu C.H., Xia M.S., Zhan X.A. and Wang M.Q. (2003). Effects of dietary fructooligosaccharide on digestive enzyme activities, intestinal microflora and morphology of male broilers. Poult. Sci. 82, 1030-1036.
Yakhkeshi S., Rahimi S. and Gharib Naseri K. (2011). The effects of comparison of herbal extracts, antibiotic, probioticand organic acid on serum lipids, immune response, GIT microbial population, intestinal morphology and performance of broilers. J. Med. Plant. 37, 80-95.
Yason C.V., Summers B.A. and Schat K.A. (1987). Pathogenesis of rotavirus infection in various age groups of chickens and turkeys: pathology. Am. J. Vet. Res. 6, 927-938.
