Determination of Nutritive Value of Poa trivialis Using in vitro Methods, Gas Production and Nylon Bag
محورهای موضوعی : Camelف. میرزائی آقجه قشلاق 1 , ا. قربانی 2 , ع. مهدوی 3 , ب. نویدشاد 4 , س. کرامتی جبه‌دار 5
1 - Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Department of Range and Watershed Management, Faculty of Agricultural Technology and Natural Resources, University
of Mohaghegh Ardabili, Ardabil, Iran
3 - Department of Animal Science, Faculty of Veterinary Medicine, University of Semnan, Semnan, Iran
4 - Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
5 - Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
کلید واژه: digestibility, degradability, metabolizabe energy, phenological growth stages, <i>Poa trivialis</i>,
چکیده مقاله :
This study was conducted to determine the chemical composition, metabolizable energy, digestibility and ability of gas production of Poa trivialis. Samples was collected at three phenological stages including: vegetative growth, flowering and seedling on two different elevations of 1300 to 1500 m and 2000 to 1800 m asl in Neor and Hir region as the first and second sites, respectively. Testing of gas production was performed using Semi-automatic gas producers machine WT-Binder 87532 Model (made in Germany). Fistulated castrated male sheeps were used for preparation of required rumen fluid. Results show that more crude protein and less cell wall were in the vegetative growth stage. Gas production was higher in the first stage in comparison with other growing stages. The amounts of gas production of soluble and insoluble structures at the first and second sites were 75.63 and 81.50 mL, respectively. Metabolizabe energy of Poa triviali was2.38 in the first site and in the second site 2.48 Mcal/kg DM in the vegetative growth stage. Collected samples from the second site had more metabolizabe energy, digestibility, degradability and gas production in comparison with the results of the first site.
این پژوهش به منظور تعیین ترکیبات شیمیایی، انرژی قابل متابولیسم، قابلیت هضم و میزان تولید گاز گونه مرتعی پوآ (Poa trivialis) صورت گرفت. نمونه‌ها در سه مرحله فنولوژیکی شامل: رشد رویشی، گل دهی و بذر دهی و در دو ارتفاع متفاوت واقع در منطقه نئور با ارتفاع 1500-1300 و منطقه هیر با ارتفاع 2000-1800 متر، به ترتیب به عنوان سایت‌های ارتفاعی اول و دوم جمع‌آوری شدند. برای انجام آزمون گاز از دستگاه نیمه اتوماتیک تولید گاز مدل WT-Binder 87532 (ساخت کشور آلمان) استفاده گردید. برای تهیه مایع شکمبه مورد نیاز از گوسفند نر اخته شده فیستولادار استفاده شد. نتایج نشان داد که در مرحله رشد رویشی نسبت به سایر مراحل، میزان پروتئین خام بیشتر و دیواره سلولی کمتر بود. میزان تولید گاز در مرحله اول رشدی در مقایسه با سایر مراحل رویشی، بیشتر بود. مقدار تولید گاز از بخش‌های محلول و نامحلول پوآ در سایت یک و دو به ترتیب 63/75 و 50/81 میلیلیتر تعیین گردید. میزان انرژی قابل متاولیسمPoa trivialis در مرحله رویشی برای سایت یک 38/2 و سایت دو 48/2 مگاکالری بر کیلوگرم ماده خشک برآورد شد. نمونه‌های گیاه جمع‌آوری شده از سایت دو دارای مقادیر انرژی قابل متابولیسم، قابلیت هضم، تجزیهپذیری و تولید گاز بیشتری نسبت به سایت اول بودند.
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