Fortification of Catalase Improves Post Thaw Fertility of Goat Semen
محورهای موضوعی : CamelR. Ranjan 1 , P. Singh 2 , C. Gangwar 3 , S.P. Singh 4 , D.K. Swain 5 , S.D. Kharche 6
1 - Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India
2 - Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India
3 - Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India
4 - Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India
5 - Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan (DUVASU), Mathura, Uttar Pradesh, India
6 - Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India
کلید واژه: Antioxidant, Catalase, Goat, mitochondrial membrane potential, post thaw semen,
چکیده مقاله :
Artificial Insemination (AI) in goats is less developed compared to the large animals due to the lack of suitable protocol of goat semen freezing and AI in India. The AI plays a pivotal role in the long-term ex-situ in vitro conservation of threatened breeds, increased productivity, and performance of a large number of non-descript and low potential goats. The objective of the present study was to enhance the life and fertility potential of cryopreserved semen and consequently the conception rate through frozen semen AI by the addition of catalase in an extender. Ejaculates (30) were collected and were extended with tris-citric acid-fructose diluent. Catalases were added in diluent (0 IU/mL, 200 IU/mL, 400 IU/mL, 600 IU/mL, 800 IU/mL and 1000 IU/mL). Analysis of data using SPSS 16 revealed that motility, live sperm count, acrosomal integrity, and hypo-osmotic swelling positive spermatozoa were counted differed significantly (P<0.05) at different concentrations of catalase. The post-thaw motility, live sperm count, acrosomal integrity, and hypo-osmotic swelling positive spermatozoa were significantly (P<0.05) highest in 800 U/mL of catalase used in the present study. Mitochondrial membrane potential was also significantly highest (P<0.05) in frozen semen in 800 IU/mL catalase. Goats (20) were inseminated with frozen semen straw of 800 IU/mL catalase and 8 goats (40%) were pregnant. The conception rate in the control group was 35%. Our study suggested that the addition of catalase reduced the detrimental effects of freezing on motility, viability, plasma membrane and acrosome integrity and can be used for routine semen freezing and AI.
Abdelhakeam A.A., Graham E.F. and Vazque I.A. (1991). Studies on the absence of glycerol in unfrozen and frozen ram semen: Fertility trials and the effect of dilution methods on freezing ram semen in the absence of glycerol. Cryobiology. 28, 36-42.
Acharya R.M. (1987). Breeds of goats and research programmes for their in India. Pp. 772-805 in Proc. 3rd Int. Conf. Goats. Brasilia, Brazil.
Aitken R.J. (2017). Reactive oxygen species as mediators of sperm capacitation and pathological damage. Mol. Reprod. Dev. 84, 1039-1052.
Azam M., Anzar M. and Arslan M. (1998). Assessment of post-thaw semen quality of buffalo and Sahiwal bulls using new semen assays. Pakistan Vet. J. 18, 74-80.
Baumber J., Vo A., Sabeur K. and Ball B.A. (2002). Generation of reactive oxygen species by equine neutrophils and their effect on motility of equine spermatozoa. Theriogenology. 57, 1025-1033.
Bilodeau J.F., Blanchette S., Cormier N. and Sirad M.A. (2002). Reactive oxygen species-mediated loss of bovine sperm motility in egg yolk Tris extender: Protection by pyruvate, metal chelators and bovine liver or oviductal fluid catalase. Theriogenology. 57, 1105-1122.
Chatterjee S. and Gagnon C. (2001). Production of reactive oxygen species by spermatozoa undergoing cooling, freezing and thawing. Mol. Reprod. Dev. 59, 451-458.
Chauhan M.S., Kapila R., Gandhi K.K. and Anand S.R. (1994). Acrosome damage and enzyme leakage of goat spermatozoa during dilution, cooling and freezing. Andrologia. 26, 21-26.
Chen S.J., Allam J.P., Duan Y.G. and Haidl G. (2013). Influence of reactive oxygen species on human sperm functions and fertilizing capacity including therapeutical approaches. Arch. Gynecol. Obstet. 288, 191-199.
Fernandes V.P., Silva M.N.N., Costa A.S., Kunkel D., Junior A.S., Feitosa L.C.S., Muratori M.C.S. and Costa A.P.R. (2018). CE inhibition in goats under fixed-time artificial insemination protocol increases the pregnancy rate and twin births. Reprod. Domest. Anim. 53, 1006-1008.
Fernandez S.M.R., Martinez P.F., Garcia M.V., Esteo M.C., Soler A.J., Paz P., Anel L. and Garde J.J. (2007). Sperm characterstics and DNA integrity of Iberian red deer (Cervus elephus) epididymal spermatozoa frozen in the presence of enzymatic and nonenzymatic antioxidants. J. Androl. 28, 294-305.
Flesch F.M. and Gadella B.M. (2000). Dynamics of the mammalian sperm membrane in the process of fertilization. Biochim. Biophys. Acta. 1469, 197-235.
Gama L.T. and Bressan M.C. (2011). Biotechnology applications for the sustainable management of goat genetic resources. Small Rumin. Res. 98(1), 133-146.
Gangwar C., Kharche S.D., Ranjan R., Kumar S., Goel A.K. and Jindal S.K. (2015). Effect of vitamin C supplementation on freezability of Barbari buck semen. Small Rumin. Res. 129, 104-107.
Gungor S., Ata A. and Inac M.E. (2018). Effects of trehalose and catalase on the viability and kinetic parameters of cryopreserved ram semen. Acta Sci. Vet. 46, 1-7.
Hancock J.L. (1951). A staining technique for the study of temperature shock in semen. Nature. 167, 323-331.
Holt W.V. (2005). Fundamental aspects of sperm cryobiology: The importance of species and individual differences. Theriogenology. 3, 47-58.
Januskauskas A. and Zillinskas H. (2002). Bull semen evaluation post-thaw and relation of semen characteristics to bull’s fertility. Vet. Zootech. 17, 39-47.
Jeferson F.F., Gilmar P.A., Joanna M.G.S., Maria E.F.O., Viviane LB., Felipe Z.B. and Olivardo F. (2017). Reproductive features and use of an anti-inflammatory drug in estrus-induced dairy goats artificially inseminated in a standing position with cervix immobilization. Reprod. Biol. 17, 268-273.
Kadirvel G., Periasamy S. and Kumar S. (2012). Effect of cryopreservation on apoptotic-like events and its relationship with cryocapacitation of buffalo (Bubalus bubalis) sperm. Reprod. Domest. Anim. 47, 143-150.
Kharche S.D., Jindal S.K., Priyadhrashini R., Kumar S., Goel A.K., Ramachandran N. and Rout P.K. (2013). Fertility following frozen semen artificial insemination in Jamunapari goats. Indian J. Anim. Sci. 83(10), 1071-1073.
Kumar D. and Naqvi S.M.K. (2014). Effect of time and depth of insemination on fertility of Bharat Merino sheep inseminated trans-cervical with frozen-thawed semen. J. Anim. Sci. Technol. 56, 8-17.
Kumar D., Kumar P., Singh P., Yadav S.P. and Yadav P.S. (2016). Assessment of sperm damages during different stages of cryopreservation in water buffalo by fluorescent probes. Cytotechnology. 68, 451-458.
Leboeuf B., Restall B. and Salomon S. (2000). Production and storage of goat semen for artificial insemination. Anim. Reprod. Sci. 62, 113-141.
Ly J., Grubb D.R. amnd Lawen A. (2003). The mitochondrial membrane potential (Dwm) in apoptosis; an update. Apoptosis. 8, 115-128.
Maneesh M. and Jayalekshmi H. (2006). Role of reactive oxygen species and antioxidants on pathophysiology of male reproduction. Indian J. Clin. Biochem. 21, 80-89.
Martin G., Cagnon N., Sabido O., Sion B., Grizard G., Durand P. and Levy R. (2007). Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa. Hum. Reprod. 22, 380-388.
Padilha R.T., Magalhaes-Padilha D.M. Cavalecante M.M., Almeida A.P., Haan K.T., Gastal M.O., Nunes J.F., Rodrigues A.P.R., Figueiredo J.R. and Oliveria M.A.L. (2012). Effect of insulin-like growth factor-I on some quality trials and fertility of cryopreserved ovine semen. Theriogenology. 78, 907-913.
Paudel K.P., Kumar S., Meur S.K. and Kumaresan A. (2010). Ascorbic acid, catalase and chlorpromazine reduce cryopreservation-induced damages to crossbred bull spermatozoa. Reprod. Domest. Anim. 45(2), 256-62.
Pietta P.G. (2000). Flavonoids as antioxidants. J. Nat. Prod. 63, 1035-1042.
Rahman A.N.M.A., Abdullah R.B. and Khadijah W.E.W. (2008). A review of reproductive biotechnologies and their application in goats. Biotechnology. 7(2), 371-384.
Ranjan R., Goel A.K., Ramachandran N., Kharche S.D., Gangwar C. and Jindal S.K. (2014). Comparison between normal and dual staining technique for evaluating acrosome status and viability in frozen thawed buck spermatozoa. Indian J. Small Rumin. 20(2), 50-53.
Ranjan R., Goel A.K., Ramachandran N., Kharche S.D. and Jindal S.K. (2015). Effect of egg yolk levels and equilibration periods on freezability of Jamunapari buck semen. Indian J. Small Rumin. 21(1), 32-36.
Ranjan R., Priyadharsini R., Goel A.K., Singh B., Kumar S., Kharche S.D. and Jindal S.K. (2017). Effect of membrane stabilizer on the freezability of buck semen. Indian J. Anim. Sci. 87(4), 435-436.
Ranjan R., Ramachandran N., Jindal S.K. and Sinha N.K. (2009a). Effect of egg yolk levels on keeping quality of Marwari buck semen at refrigeration temperature. Indian J. Anim. Sci. 79(7), 662-664.
Ranjan R., Ramachandran N., Jindal S.K. and Sinha N.K. (2009b). Hypo osmotic swelling test in frozen thawed goat spermatozoa. Indian J. Anim. Sci. 79(10), 1022-1023.
Revell S.G. and Mrode R.A. (1994). An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36, 77-86.
Roca J., Rodriguez M.J., Gil M.A., Carvajal G., Garcia E.M. and Cuello C. (2005). Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and / or catalase. J. Androl. 26, 15-24.
Salamon S. and Maxwell W.M.C. (1995). Frozen storage of ram semen II. Causes of low fertility after cervical insemination and methods of improvement. Anim. Reprod. Sci. 38, 1-36.
Saraswat S., Jindal S.K., Ramachandran N., Yadav S. and Priyadarshini R. (2012). Standardization of antioxidants fortification in frozen buck semen. Indian J. Small Rumin. Res. 18 (1), 1-11.
Selvaraju S., Ravindra J.P., Ghosh J., Gupta P.S.P. and Suresh K.P. (2008). Evaluation of sperm functional attributes in relation to in vitro sperm-zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality. Anim. Reprod.. Sci. 106, 311-321.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 20. Chicago SPSS Inc., USA.
Thiangtum K., Hori T. and Kawakami E. (2012). Effect of catalase and superoxide dismutase on motility, viability and acrosomal Integrity of canine spermatozoa during storage at 5. Thai J. Vet. Med. 42(4), 447-453.
Watson P.F. (1975). Use of Giemsa stain to detect changes in acrosome of frozen ram spermatozoa. Vet. Rec. 97, 12-15.
White I.G. (1993). Lipids and calcium uptake of sperm in relation to cold shock and preservation. Reprod. Fertil. Dev. 5, 639-658.
Witte T.S. and Schafer-Somi S. (2007). Involvement of cholesterol, calcium and progesterone in the induction of capacitation and acrosome reaction of mammalian spermatozoa. Anim. Reprod. Sci. 102, 181-193.
