Expression Profile of Five Stress-Related Genes of Khorasan Native Chickens under Acute Heat Stress
محورهای موضوعی : Camelر. توحیدی 1 , م.ر. نصیری 2 , ع. جوادمنش 3 , آ. جوانمرد 4
1 - گروه علوم دامی - مجتمع آموزش عالی تربت جام - خراسان رضوی
2 - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
کلید واژه: Gene expression, heat stress, heat shock protein, Khorasan native chicken,
چکیده مقاله :
High temperature is one of the main environmental factors causing economic losses to the poultry industry, as it reduces growth and production performance of chickens. The heat shock proteins (HSPs) play a key role in cellular defense mechanisms during exposure in a hot environment. The aim of this study was to evaluate the expression level of the candidate genes in the liver of Khorasan native chickens under acute heat stress. Sixteen 42 days old chickens were divided into two groups; the control (25 ˚C and 50% humidity) and heat-treated (42 ˚C and 50% humidity), and then the liver was sampled. The level of gene expression of HSPB1, HSPB9, SERPINH1, HSPA2 and HSP110 were evaluated using the reverse transcription- quantitative polymerase chain reaction (RT-qPCR) method. The results of the analysis of variance revealed that the expression of HSPA2 and HSP110 was significantly increased. In the biological processes of gene ontology, three processes had FDR < 0.01. HSPA2 and HSPB1 involved in the processes that stimulated cells against increasing temperature. The results indicated that Khorasan native chickens have suitable resistance to acute heat stress. Furthermore, HSPA2 has the ability to express under high ambient temperature in order to protect the structure of cellular proteins.
دمای بالا یکی از فاکتورهای اصلی محیطی مؤثر بر کاهش سود اقتصادی واحدهای پرورش طیور است زیرا، رشد و تولید پرنده را مختل میکند. پروتئینهای شوک گرمایی (HSPs) نقش کلیدی در مکانیسم دفاعی سلولی علیه گرمای محیطی دارند. هدف از انجام مطالعه حاضر، بررسی میزان بیان ژنهای کاندیدا در کبد مرغان بومی خراسان تحت تنش گرمایی حاد بود. برای این منظور 16 جوجه 42 روزه به دو گروه تقسیم شدند؛ شاهد (دمای محیطی °C25 و رطوبت 50 درصد) و تحت تنش گرمایی (دمای محیطی °C42 و رطوبت 50 درصد)، و سپس از کبد آنها نمونهبرداری شد. سطح بیان ژنهای HSPB1، HSPB9، SERPINH1، HSPA2 و HSP110 با روش RT-qPCR اندازهگیری شد. نتایج تجزیه واریانس نشان داد که بیان ژنهای HSPA2 و HSP110 به طور معنیداری افزایش داشت. سه فرایند از فرایندهای بیولوژیکی هستیشناسی ژنی دارای FDR کمتر از 0.01 بودند. ژنهای HSPA2 و HSPB1 در این فرایندها شرکت داشتند که باعث تحریک سلولها در برابر افزایش دما میشدند. این نتایج نشان میدهند که مرغان بومی خراسان مقاومت مطلوبی در برابر تنش گرمایی حاد دارند. همچنین، ژن HSPA2توانایی بیان زیاد در دمای محیطی بالا برای حفظ ساختار پروتئینهای سلولی را دارد.
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