Effect of Pelleting Temperature, Probiotic and Wheat Grain on Growth Performance, Blood Biochemical Variables, Immune Responses and Mucin 2 Gene Expression of Broiler Chicks
محورهای موضوعی : Camelو. ایقانی 1 , ع.ا. صادقی 2 , س.ن. موسوی 3 , پ. جعفری 4 , م. چمنی 5
1 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Animal Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran
4 - Department of Microbiology, Arak Branch, Islamic Azad University, Arak, Iran
5 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
کلید واژه: broiler chicks, Cholesterol, immune responses, mucin 2,
چکیده مقاله :
Conditioning is a major stage in the pelleting process for feeds. Probiotics are known to have the ability for improvement of the immune system and growth performance. Wheat can be used as a replacement for corn. Thus, the present study was conducted to investigate the effects of pelleting temperature, probiotics and wheat grain on growth performance, blood biochemical variables, immune responses and mucin 2 gene expression of broiler chicks. Eight-hundred 1-d-old broiler chicks were assigned into 8 treatments with 4 replicates (n=25 chicks). A completely randomized design in a 2 × 2 × 2 factorial arrangement was used with two levels of wheat (0 and 500 g/kg), two levels of probiotic (0 and 200 mg/kg) and two levels of conditioning temperature (70 or 85 ˚C). Growth performance, immune responses, blood biochemical variables, and mucin 2 gene expression were evaluated. Main and interaction effects were not significant on growth performance, immune responses and blood biochemical variables (P>0.05). Wheat and corn-based diets containing probiotic up-regulated mucin 2 gene expression (p < 0.05). Wheat can be used as an alternative for corn.
کاندیشنینگ، یک مرحله اصلی در فرآیند پلت کردن خوراک محسوب میگردد. پروبیوتیکها با داشتن توانایی بهبود عملکرد سیستم ایمنی و عملکرد رشد شناخته میشوند. گندم میتواند به عنوان یک جایگزین برای ذرت مورد استفاده قرار گیرد. از این رو، مطالعه حاضر با هدف بررسی دمای پلت، پروبیوتیک و دانه گندم بر عملکرد رشد، متغیرهای بیوشیمیایی خون، پاسخ سیستم ایمنی و بیان ژن موسین 2 در جوجههای گوشتی انجام گرفت. تعداد 800 قطعه جوجه یکروزه گوشتی در قالب هشت تیمار و چهار تکرار برای هر تیمار تقسیمبندی شدند (به ازای هر تکرار 25 قطعه). طرح آزمایشی کاملا تصادفی به صورت فاکتوریل 2 × 2 × 2 براساس دو سطح مصرف گندم (0 و 500 گرم بر کیلوگرم)، دو سطح مصرف پروبیوتیک (0 و 200 میلیگرم بر کیلوگرم) و دو دمای کاندیشنینگ (70 و 85 درجه سلسیوس) مورد استفاده قرار گرفت. عملکرد رشد، پاسخ سیستم ایمنی، متغیرهای بیوشیمیایی خون و بیان ژن موسین 2 مورد اندازهگیری قرار گرفت. در رابطه با عملکرد رشد، پاسخ سیستم ایمنی و متغیرهای بیوشیمیایی خون، اثرات اصلی و متقابل معنیدار نبودند (P<0.05). جیرههای محتوی پروبیوتیک هم بر پایه ذرت و هم بر پایه گندم، باعث افزایش بیان ژن موسین 2 شدند (P<0.05). گندم میتواند به عنوان یک جایگزین برای ذرت مورد استفاده قرار بگیرد.
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