Identification of Pseudohermaphrodite Polled Apparently Female Goats Based on ZFX and SRY Sex Determination
محورهای موضوعی : CamelH. Abyar 1 , B. Qasemi-Panahi 2 , G. Moghaddam 3 , S.A. Rafat 4 , A. Javanmard 5
1 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
5 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
کلید واژه: Goat, intersexuality, polled intersex syndrome, <i>SRY</i>, <i>ZFX</i>,
چکیده مقاله :
The presence of intersexuality in goat breeds is one of the most popular, and challenging issues. Therefore, understanding the genetic complexity of this phenomenon at the DNA level may help to discover the molecular mechanisms of sexual differentiation in this species. A solution to this problem is proposed in the development of a DNA test using sex-determination candidate genes. With this motivation, the main goal of the present report was to identify the pseudohermaphrodite polled goats based on ZFX and SRY sex-determination candidate gene findings. Overall, data were collected from 11 individuals (4 females, 4 males, and 3 anatomically female goats (intersex) were chosen for PCR analysis using ZFX and SRY specific primers sequence. For this reason, genomic DNA extraction and PCR amplification were done according to routinely available laboratory protocol. Two ZFX and SRY-specific primer sets were applied for PCR amplification. Interestingly, our results were re-confirmed, and PCR products were amplified by ZFX primer yielding a band of 445 bp in both female and intersex goats. Furthermore, SRY expressed 169 bp in both male and intersex goats. In summary, these results suggest that SRY candidate gene may provide insight into the factors affecting the intersexuality issue in goat breeds.
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