Immune Responses and some Blood Metabolite Responses of Female Holstein Calves to Dietary Supplementationwith Licorice Root (Glycyrrhiza glabra)
محورهای موضوعی : CamelR. Sajjadi 1 , A.A. Solati 2 , M. Khodaei Motlagh 3 , M. Kazemi Bonchenari 4
1 - Department of animal science, Saveh Branch, Islamic Azad University, Saveh, Iran
2 - Department of animal science, Saveh Branch, Islamic Azad University, Saveh, Iran
3 - Department of animal science, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
4 - Department of animal science, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran
کلید واژه: blood metabolites, Holstein calf, immune responses, licorice,
چکیده مقاله :
Different medicinal plants have been used in animal and poultry nutrition in last decade. It seems that there are some potential to these medicinal plants to replace with common antibiotics. Licorice root (Glycyrrhiza glabra) is a medicinal plant that extensively was used in different studies. In the present study, the effect of dietary addition of licorice root on performance and blood energy indicator concentrations (glucose, non-esterified fatty acid (NEFA), beta hydroxyl butyrate (BHB) and immune responses parameters (total immunoglobulin (IG), IgG and IgA) was evaluated on female Holstein calves. The fourteen female Holstein calves with average body weight of 85.5 kg were subjected to two different treatments (seven animals per each treatment) in a completely randomized design. The control treatment was considered as C and supplemented treatment with licorice was considered as L. The study lasted ten weeks. Of which the first week was considered as adaptation period. Feed intake was measured daily and blood sample were collected twice throughout the experiment. The results show that average dry matter intake (DMI) was 1790 and 1860 g/d for treatments C and L, respectively (P>0.05). The NEFA was different significantly between treatments (P<0.05). The IgA concentration was not differed between treatments; however both total Ig and IgG concentrations were positively increased by licorice supplementation treatments (P<0.05). Total Ig concentration was 105.1 and 138.2 mg/dL for treatments C and L, respectively which shows that licorice addition caused to 31% increase in immunity responses compared to control treatment. In conclusion the results show that although licorice supplementation did not affect performance of animal, but it has potential to improve energy status in dairy calves' nutrition and also increased immune response of animal.
در دهه اخیر گیاهان دارویی متفاوتی در تغذیه دام و طیور مورد استفاده قرار گرفته است. به نظر می‌رسد امکان جایگزینی برخی از گیاهان دارویی به جای آنتی‌بیوتیک‌های وسیع الطیف وجود دارد. ریشه شیرین بیان یک گیاه دارویی محسوب می‌شود که در مطالعات متفاوتی مورد استفاده قرار گرفته است. در مطالعه حاضر تأثیر افزودن ریشه شیرین بیان بر عملکرد و شاخص‌های انرژی (گلوکز، اسیدهای چرب غیر استریفه و بتاهیدروکسی بوتیرات) و عملکرد سیستم ایمنی (ایمنوگلوبین کل، ایمنوگلوبین G و ایمنوگلوبین A) گوساله‌های ماده هولشتاین مورد بررسی قرار گرفت. تعداد 14 رأس گوساله ماده با میانگین وزن 5/85 کیلوگرم در قالب طرح کاملاً تصادفی و در 2 تیمار قرار گرفتند (7 رأس گوساله در هر تیمار). تیمار شاهد به عنوان تیمار C در نظر گرفته شد و تیماری که مکمل شیرین بیان دریافت کرده بود به عنوانL در نظر گرفته شد. آزمایش 10 هفته در نظر گرفته شد. هفته اول به عنوان دوره عادت دهی در نظر گرفته شد. مصرف خوراک به صورت روزانه بررسی شد و نمونه خون دو بار در طول آزمایش اندازه‌گیری شد. نتایج نشان داد که میانگین مصرف ماده خشک برابر 1790 و1860 گرم در روز برای تیمارهای C و L بود (05/0<P). غلظت اسیدهای چرب استریفه نشده در بین دو تیمار تفاوت معنی‌داری نشان داد (05/0P<). غلظت ایمنوگلوبین A تفاوت معنی‌داری نشان نداد اما غلظت کل ایمنوگلوبین‌ها و ایمنوگلوبین G تفاوت معنی‌داری در بین دو تیمار نشان دادند (05/0P<). غلظت کل ایمنوگلوبین‌ها برابر 1/150 و 2/138 میلی‌گرم در دسی‌لیتر برای تیمارهای C وL بود که نشان دهنده بالاتر بودن این فراسنجه تا حدود 31 درصد با استفاده مکمل در برابر تیمار شاهد بود. به طور خلاصه نتیجه‌گیری شد با وجودی که مکمل شیرین بیان عملکرد دامها را تحت تأثیر قرار ندادده بود اما این مکمل توانست وضعیت انرژی در گوساله‌ها را بهبود داده و همچنین وضعیت ایمنی را در این دامها بهبود دهد.
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