Evaluation of Total Antioxidant, Total Calcium, Selenium, Insulin, Free Triiodothyronine and Free Thyroxine Levels in Cows with Ketosis

محورهای موضوعی : Camel

اس. کوزت ¹ ( Department of Internal Medicine, Faculty of Veterinary, Yuzuncu Yıl University, Zeve Campus, Van, Turkey )
ن. یوکسک ² ( Department of Internal Medicine, Faculty of Veterinary, Yuzuncu Yıl University, Zeve Campus, Van, Turkey )

تاریخ دریافت : 1395/11/10 تاریخ پذیرش : 1396/01/11 تاریخ انتشار : 1396/06/10

کلید واژه: dairy cows, Calcium, insulin, thyroid, selenium, ketosis,

چکیده مقاله :

Ketosis is an important metabolic disease of high milk-producing cows. There are significant changes in many metabolite and hormonal concentrations in metabolic diseases. This study was carried out to assess the concentrations calcium (Ca), selenium (Se), total antioxidant (TAOC), insulin, free triiodothyronine (fT3) and free thyroxine (fT4) in cows with subclinical and clinical ketosis. This study included 20 dairy cows within the first two months of lactation, aged between 4-8 years. Cows with ß-hydroxybutyrate acid (BHBA) concentrations 1.20 mmol/L were considered healthy, whereas 1.20 and 1.50 mmol/L were considered subclinical and 1.60-2.20 mmol/L were classified as clinically ketotic. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT),low density lipoprotein (LDH), glucose, Ca, plasma TAOC capacity and BHBA concentrations were performed spectrophotometrically. Serum insulin, free triiodothyronine and free thyroxine concentrations were measured using the chemi-luminescence method. Serum Se concentrations were measured using an Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). In conclusion, significant changes were noted in decreased concentrations of TAOC, Ca, Se, fT3, fT4 and insulin in cows with subclinical and clinical ketosis. The study identified important parameters, changes in the levels of these parameters will be important in determining the treatment and prognosis of the disease. Their use may also help reduce the economic losses suffered by dairy farmers as a result of the disease.

چکیده انگلیسی:

کتوز یک بیماری متابولیکی مهم در گاوهای پرتولید است. تغیرات معنی­داری در غلظت اکثر متابولیت­ها و هورمن­ها در بیماری­های متابولیکی اتفاق می­افتد. این مطالعه با هدف سنجش غلظت­های کلسیم (Ca)، سلنیوم (Se)، آنتی­اکسیدان کُل (TAOC)، انسولین، تری یدو تیرونین آزاد (fT3) و تیروکسین آزاد (fT4) در گاوهای مبتلا به کتوز تحت حاد و حاد صورت گرفته است. در این مطالعه 20 گاو شیرده در دو ماه نخست دوره شیردهی و با سن بین 4 تا 8 سال مورد مطالعه قرار گرفته است. گاوهایی که غلظت بتاهیدروکسی بوتیرات اسید (BHBA) در آنها 20/1 میلی­مولار در هر لیتر بوده، سالم تلقی شده، آنهایی که دارای غلظت بین 20/1 تا 50/1 میلی­مولار در هر لیتر بودند، تحت حاد محسوب شده و آنهایی که دارای غلظت 60/1 تا 20/2 میلی­مولار در هر لیتر بودند، در دسته مبتلایان به کتوز حاد دسته­بندی گردیدند. آسپارتات آمینو ترانسفراز سرمی (AST)، آلانین آمینو ترانسفراز (ALT)، لیپوپروتئین با چگالی پایین (LDH)، گلوکز، کلسیم، ظرفیت TAOC پلاسمایی و غلظت BHBA با استفاده از اسپکتروفتومتری اندازه­گیری گردید. غلظت انسولین سرم، تری یدو تیرونین آزاد و تیروکسین آزاد نیز با استفاده از روش کمی­لومینسنس اندازه­گیری شد. غلظت Se سرمی با استفاده از اسپکتروفتومتری القایی پلاسما توده جفت شده (ICP-MS) مورد سنجش قرار گرفت. نتایج نشان داد که غلظت TAOC، Ca، Se، fT3، fT4 و انسولین در گاوهای مبتلا به کتوز تحت حاد و حاد کاهش می­یابد. این مطالعه، نشان داد که پارامترهای مهم و تغییرات در سطوح این پارامترها تعیین‌کننده درمان و پیشگیری از بیماری کتوز هستند. این موضوع می­تواند به کاهش ضررهای اقتصادی ناشی از بیماری کتوز در مزارع پرورش گاو شیری کمک کند.

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