Molecular Evaluation of the Myostatin Gene in Four Iranian Native Goat Breeds Did not Demonstrate any Association with Twining Rate
محورهای موضوعی : Camelع. عبدالمحمدی 1 , ک. خانی 2 , ص. فروتنی فر 3 , ع. زبرجدی 4 , ل. سیمایی 5 , م. گلی 6
1 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
2 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
3 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
4 - Department of Agronomy Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
5 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
6 - Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
کلید واژه: genetic diversity, Goat, myostatin, twining,
چکیده مقاله :
The objective of this study was to evaluate myostatin (MSTN) gene by polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) and sequencing methods in four goat breeds including Mohabadi, Markhoz, Lori and Bital breeds. A573 bp fragment of MSTN gene was amplified by PCR. A new 5 bp deletion in 5`UTR (206TTTTA) of the MSTN gene was directly identified by sequencing. The AA and AB genotypes were observed in all above breeds, while the BB genotype was found only in Mohabadi breed. The AA genotype had the highest genotypic frequency in all breeds. On the other hand, the BB genotype in Mohabadi goat breed had the lowest genotypic frequency. The A allele frequency was higher than that of the B allele in the four breeds investigated in this study. The A allele frequency significantly differed between two (litter size) subgroups in Mohabadi breed. This difference in the allele frequency was not significant in Lori and Mohabadi breeds. Therefore, odds ratios statistics (ORs) showed no significant differences between genotypes for twining rate. Evaluation of MSTN gene 5`UTR region demonstrated the presence of two TATA boxes (important putative regulatory elements) which are located in the promoter region (position 32-36, 56-60) and one E-box (position 42-48) motifs. In conclusion, our results showed that the 5`UTR region polymorphism cannot be a key factor in the occurrence of twining in the above native goat breeds. It seems that more studies in large populations are needed to investigate the effect of MSTN gene on twining with more focus on other mutations in MSTN gene or other genes.
هدف این مطالعه ارزیابی ژن میوستاتین در چهار نژاد بز شامل مهابادی، مرغز، لری و بیتال با روشهای PCR-RFLP و توالییابی بود. یک قطعه 573 جفت بازی از ژن میوستاتین با PCR تکثیر شد. با روش توالییابی، یک حذف شدگی 5 جفت بازی (206TTTA) در ناحیه 5`UTR ژن میوستاتین شناسایی گردید. ژنوتیپهای AA و AB در همه نژادهای فوق، ولی ژنوتیپهای BB فقط در نژاد مهابادی یافت شدند. ژنوتیپ AA دارای بالاترین فراوانی در همه نژادها بود. از سوی دیگر، ژنوتیپ BB در نژاد مهابادی دارای کمترین فراوانی بود. در چهار نژاد بز مورد بررسی در این مطالعه، فراوانی آلل A بالاتر از آلل B بود. فراوانی آلل A در دو زیرگروه نژاد مهابادی (برای تعداد بزغاله در زایش) تفاوت معنیداری داشت. این تفاوت در فراوانی آللی در نژادهای لری و مرغز معنیدار نبود. همچنین آمارههای نسبت بخت (ORs) برای نرخ دوقلوزایی، تفاوتهای معنیداری را بین ژنوتیپها نشان نداد. ارزیابی ژن میوستاتین حضور دو جعبه TATA (عناصر مهم تنظیمی) در ناحیه پروموتور (موقعیتهای60-56، 32-36)، و یک جعبه E (موقعیت 42-48) را نمایان کرد. به طور کلی، نتایج ما نشان داد که چندشکلی در ناحیه 5`UTR ژن میوستاتین نمیتواند یک فاکتور کلیدی مهم در بروز دوقلوزایی در این نژادهای بومی بز باشد. به نظر میرسد مطالعات بیشتری در جمعیتهای دامی بزرگ جهت بررسی تأثیر ژن میوستاتین روی دوقلوزایی، با توجه بیشتر روی جهشهای دیگر ژن میوستاتین یا ژنهای دیگر، مورد نیاز است.
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