Polymorphism in FSHβ and FSHRGenes and Their Relationship with Productive and Reproductive Performance in Iran Black, Arman and BaluchiSheep Breeds
محورهای موضوعی : Camelن. نظیفی 1 , ق. رحیمی میانجی 2 , ز. انصاری پیر سرایی 3
1 - Department of Animal Science, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
2 - Department of Animal Science, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
3 - Department of Animal Science, Faculty of Animal and Aquatic Science, Sari Agricultural Science and Natural Resources University, Sari, Iran
کلید واژه: FSH&beta, , FFSHR, polymorphism, litter size, body weight,
چکیده مقاله :
The objective of the present study was to detect polymorphism in follicle stimulating hormone receptor (FSHR) and beta subunit of follicle stimulating hormone (FSHβ) genes and their relation to litter size and body weight trait in Baluchi, Iran black and Arman sheep breeds. PCR-RFLP technique using ACCI and HinfI enzyme treatments employed to detection of polymorphism in FSHβ marker site but detected no polymorphism pattern in this locus. SSCP analysis used for more survey on detection of polymorphism in FSHβ marker site which resulted in AA and AC genotypes in Baluchi sheep, AA and AB genotypes in Iran black population and no polymorphism pattern detected in Arman breed and all samples showed AA genotype. Polymorphism in FSHR gene has been detected by treatment with MSCI restriction enzyme. Three genotypes of AA, AB and BB were observed among studied breeds. The B allele was found with the highest frequency in all tree breeds. Analysis of variance for the effects of theses marker loci was carried out using SAS program. Mean comparison by Duncan test showed that the effect of each FSHβ and FSHR marker sites on litter size has significant effect only in Baluchi sheep population. It seems mutant alleles can improve considerably mean of litter size than the wild type ones. Analysis of data for body weight and polymorphisms in different ages showed that there are no significant differences between genotypes of FSHβ locus and body weight trait in various ages in none of Baluchi, Iran-black and Arman breeds. Survey on polymorphisms of FSHR locus and body weight trait revealed significant difference in some ages only in Arman breed and wild type allele caused better performance. This information can be used to plan breeding programs for sheep breeds aimed at improving production and reproduction performance.
هدف از مطالعه حاضر بررسی چند شکلی در ژن گیرنده هرمون محرک فولیکولی (FSHR) و ژن زیر واحد بتای هرمون محرک فولیکولی (FSHβ) و ارتباط آن با صفات نرخ زایش و افزایش وزن بدن در گوسفندان نژاد بلوچی، ایران بلک و آرمان می‌باشد. در ابتدا ردیابی چند شکلی در ژن FSHβ به کمک تکنیک PCR-RFLP و آنزیم‌های محدود کننده ACCI و HinfI انجام شد و هیچ نوع الگوی باندی چند شکلی در این جایگاه ژنی نشان داده نشد. در مرحله بعد به منظور مطالعات بیشتر در این جایگاه ژنی از تکنیک SSCP استفاده شد که در نتیجه آن ژنوتیپ‌های AA و AC در گوسفندان نژاد بلوچی، ژنوتیپ‌های AA و AB در گوسفندان نژاد ایران بلک مشاهده شد ولی کلیه نمونه‌های مربوط به گوسفندان نژاد آرمان فقط ژنوتییپ AA را نشان دادند. ردیابی چند شکلی در ژن FSHR به کمک تکنیک PCR-RFLP و تیمار آنزیمی MSCI انجام شد و سه نوع ژنوتیپ AA، AB و BB در بین نژادهای مورد مطالعه مشاهده شد. آلل B در هر سه نژاد مورد مطالعه بیشترین فراوانی را داشت. آنالیز تجزیه واریانس این جایگاه‌های ژنی به کمک نرم افزار SAS انجام شد. آزمون مقایسه میانگین دانکن نشان داد که چند شکلی‌های هر دو جایگاه ژنی FSHR و FSHβ تأثیر معنی داری را روی صفت نرخ زایش در نژاد بلوچی دارند. همچنین نشان داد که آلل جهش یافته میتواند متوسط نرخ زایش را به صورت قابل توجهی نسبت به آلل وحشی افزایش دهد. آنالیز داده‌های مربوط به صفت وزن بدن در سنین مختلف و چند شکلی در جایگاه ژنی FSHβ، در هیچ یک از سه نژاد مورد مطالعه اختلاف معنی داری را نشان نداد. مطالعات چند شکلی در جایگاه ژنی FSHR و صفات افزایش وزن بدن نشان دهنده وجود اختلاف معنی داری در نژاد آرمان است، و نشان داد که آلل وحشی عملکرد بهتری دارد. این اطلاعات میتواند در برنامه‌های اصلاح نژادی در پرورش گوسفند مورد استفاده قرار بگیرد و سبب بهبود عملکرد تولیدی و تولید‌مثلی شود.
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