Genotypic and Allelic Frequencies of IGF1 and IGF2 Genes in Broilers Analysed by Using PCR‐RFLP Techniques
محورهای موضوعی : CamelT. Shah 1 , S. Deshpande 2 , U. Dasgupta 3 , K.M. Singh 4 , C.G. Joshi 5
1 - Department of Animal Biotechnology, College of Veterinary Science and A.H. Anand Agricultural University, Anand, 388001, Gujarat, India
2 - Marshall Breeders Pvt Limited, C and M House, N.D. Patel R.d. Nasik, 422001, Maharashtra, India
3 - Marshall Breeders Pvt Limited, C and M House, N.D. Patel R.d. Nasik, 422001, Maharashtra, India
4 - Department of Genetics, ARIBAS, New V.V. Nagar, Anand, India
5 - Department of Animal Biotechnology, College of Veterinary Science and A.H. Anand Agricultural University, Anand, 388001, Gujarat, India
کلید واژه: PCR-RFLP, genotype, <i>IFG1</i>, <i>IGF2</i>,
چکیده مقاله :
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B and three types of genotypes of AA, AB and BB were observed in both genes. The results indicated predominant B-allele (0.85) was higher for IGF1 butA-allele (0.5611) IGF2 in the broiler population.
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