Survey of FecXL Locus of BMP15 Gene and Growth Hormone (GH) Gene and Their Effects on Lambing Rate in Zel Sheep
محورهای موضوعی : Camel
S. Yousefi
1
(Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
L. Shahmohammadi
2
(Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
M. Ahani Azari
3
(Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
S. Zerehdaran
4
(Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
E. Dehnavi
5
(Department of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran)
کلید واژه: PCR-RFLP, Afshari, MHC, Ovar-DRB1, Zel,
چکیده مقاله :
The GH gene and BMP15 gene have been used as candidate genes for marker-assisted selection in different livestock species. Random blood samples were obtained from 180 Zel sheep breed to study genetic polymorphism of these genes. DNA was extracted from blood samples and a 365 bp fragment from the exon V of the ovine growth hormone gene and a 312 bp region from exon II of BMP15 gene were amplified by polymerase chain reaction. SSCP analysis showed three conformational patterns (A, B and C) for GH gene but for FecXL locus of BMP15 no banding patterns were observed in the animals tested. Results indicated that there were no significant associations (P>0.05) between polymorphism of the GH loci and lambing rate.
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