Polymorphism of DGAT1 Gene and Its Relationship with Carcass Weight and Dressing Percentage in Moghani Sheep Breed

محورهای موضوعی : Camel

ف. علاء نوشهر ¹ ( Department of Animal Science, University of Tabriz, Tabriz, Iran )
ع. رأفت ² ( Department of Animal Science, University of Tabriz, Tabriz, Iran )

تاریخ دریافت : 1392/05/05 تاریخ پذیرش : 1392/07/08 تاریخ انتشار : 1393/03/11

کلید واژه: polymorphism, Moghani sheep, DGAT1 gene, carcass weight, dressing percentage,

چکیده مقاله :

The diacylglycerol acyltransferase 1 gene (DGAT1) was identified as a strong candidate gene affecting mutton quality traits in sheep. Single nucleotide polymorphism creates a single base mutation (C to T) in AGCT site of endonuclease AluI. DGAT1 is one of the candidate genes to improve carcass characteristics in feedlot animals. In order to study area T487C in exon 17 of the DGAT1 polymorphism, Iranian Moghani sheep breeds randomly slaughtered in the abattoir were recorded. DNA was extracted from 150 samples of Moghani sheep. Polymerase chain reaction to amplify 309 bp of exon 17 DGAT1 gene using a pair of specific primers was performed. Genotypes obtained from method PCR-RFLP and directly from agarose gel. Two alleles T and C with frequencies of 0.829 and 0.171 were observed respectively. Statistical analysis showed polymorphism in exon 17 region of the gene significantly correlated with carcass weight and dressing percentage (P<0.05). So that the CC genotypes of the significant mean carcass weight and dressing percentage heavier than had TT genotypes (P<0.05). Of polymorphism can be observed that improvement in breeding programs to improve carcass weight and dressing percentage through selection in favor of superior genotypes be used.

چکیده انگلیسی:

ژن دی آسیل گلیسرول آسیل ترانسفراز (DGAT1) به عنوان ژن کاندیدا برای صفات کیفی گوشت گوسفند شناخته شده است. یک چند شکلی تک نوکلئوتیدی منجر به ایجاد جهش (C به T) در جایگاه AGCT آنزیم اندونوکلئاز AluI می‌شود. به منظور مطالعه چند شکلی ناحیه T487C در اگزون 17 ژن DGAT1 به طور تصادفی از 150 گوسفند مغانی، خون­گیری و استخراج dna به عمل آمد. واکنش زنجیره­ای پلیمراز برای تکثیر قطعه 309 جفت بازی از اگزون 17 ژن DGAT1 با استفاده از یک جفت آغازگر اختصاصی انجام گرفت. نتایج آنالیز PCR-RFLP نشان داد که SNP دارای سه ژنوتیپ TT (37 و 272bp ) ، TC (37، 272 و 309 bp) و CC (309bp ) می­باشد. همچنین فراوانی دو آلل T و C به ترتیب برابر با 829/0 و 171/0 مشاهده شد. آنالیز آماری ارتباط معنی­داری را بین صفات وزن دنبه و ضخامت چربی پشت با ژنوتیپ­ها نشان داد. به طوری‌که گوسفندان دارای ژنوتیپ CC به طور معنی­داری (05/0P<) دنبه سنگین­تر و ضخامت چربی بیشتری نسبت به گوسفندان با ژنوتیپTT ، داشتند. در نتیجه از چند شکلی نشان داده شده ممکن است بتوان در برنامه­های اصلاح نژادی گوسفند مغانی از طریق انتخاب ژنوتیپ­های برتر، برای بهبود صفات وزن لاشه و بازده لاشه بهره جست.

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