Iron Loaded Chitooligosaccharide Nanoparticles Reduces Incidence of Bacterial Chondronecrosis with Osteomyelitis in Broiler Chickens
محورهای موضوعی : Camel
1 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
2 - Department of animal science, Faculy of agriculture, Bu-Ali Sina University
کلید واژه: broiler chicken, lameness, Iron, bacterial chondronecrosis, chitooligisaccharide,
چکیده مقاله :
The current study was conducted to investigate the effects of dietary iron-loaded chitooligosacharide nanoparticles (Fe-CNP) on occurrence of bacterial chondronecrosis with osteomyelitis (BCO) in broiler chickens. Four hundred eighty day-old chicks (Ross 308) were assigned to four treatments with six replicates of 20 birds. All chicks were reared on wire flooring system up to 42 days of age. The control diet was formulated to meet the Aviagen recommendations except for iron supplement. The experimental groups were contain: 10 mg/kg Fe from Fe-CNP (group Fe-CNP), 20 mg/kg Fe from FeSO4 (group FeSO4) and 20 mg/kg Fe from FeSO4 + CNP (group FeSO4+CNP). Results showed a significant increase in production efficiency factor, weight gain and FCR in all Fe supplemented groups (P<0.05) compared to the control. Feed intake was not differed significantly between groups (P>0.05). Serum tumor necrosis factor-alpha (TNF-α) level decreased significantly by Fe-CNP, whereas serum immunoglobulin G (IgG) level increased significantly in this group (P<0.05). The prevalence of impaired gait scores (GS≥3) in live birds in day 42 did not show a significant difference between dietary treatments (P>0.05). Lame broilers evaluation showed that lower control and Fe-CNP had lower impaired gaits (P<0.05). Supplementation of FeSO4 significantly increased the percentage of femur and tibia lesions (P<0.05). Leg evaluation of live broilers showed that Fe-CNP and control groups exhibited a higher incidence of normal femur (NF) and a lower percentage of transitional femur necrosis (FHT) and total femur lesions (All F; P<0.05). Femoral head necrosis (FHN) and FHS were not affected significantly by dietary treatments (P>0.05). Totally, Fe-CNP supplement, by improving immune system, reduced the prevalence of BCO lesions in broiler chickens.
مطالعه حاضر برای بررسی اثرات جیره نانوذرات چیتولیگوساکارید انباشته از آهن (Fe-CNP) روی وقوع کندرونکروزیس باکتریایی با پوکس استخوان (BCO) در جوجه‌های گوشتی انجام شد. 400 جوجه هشت روزه (Ross 308) به چهار تیمار با شش تکرار از 20 پرنده اختصاص یافتند. تمامی جوجه‌ها روی سیستم کفپوش سیمی تا سن 42 روزگی پرورش یافتند. جیره شاهد براساس توصیه‌های Aviagen به غیر از مکمل آهن فرموله شده بودند. گروه‌های آزمایشی شامل: 10 میلی‌گرم/کیلوگرم Fe از Fe-CNP (گروه Fe-CNP)، 20 میلی‌گرم/کیلوگرم Fe از FeSO4(گروه FeSO4) و 20 میلی‌گرم/کیلوگرم Fe از FeSO4 + CNP (گروه FeSO4 + CNP) بودند. نتایج یک افزایش معنی‌دار در فاکتور بهره‌وری تولید، رشد وزنی و FCR در تمامی گروه‌های مکمل‌سازی شده با Fe (05/0P<) در مقایسه با شاهد نشان دادند. خوراک مصرفی تفاوت معنی‌داری بین گروه‌ها نداشت (05/0P<). سطح فاکتور آلفا نکروزیس تومور پلاسما (TNF-α) به طور معنی‌داری توسط Fe-CNP کاهش یافت، اگرچه سطح ایمونوگلوبولین G (IgG) سرم به طور معنی‌داری در این گروه افزایش یافت (05/0P<). شیوع امتیازات راه رفتن با اختلال (3GS≥) در پرندگان زنده در روز 42 تفاوت معنی‌داری بین تیمارهای جیره‌ای نشان نداد (05/0P<). ارزیابی جوجه‌های لنگ شده نشان داد که شاهد و Fe-CNP کمتر اختلالات راه رفتن کمتری داشتند (05/0P<). مکمل FeSO4به طور معنی‌داری درصد آسیب‌های استخوان‌های femur و tibia را افزایش داد (05/0P<). ارزیابی پای جوجه‌های زنده نشان داد که گروه‌های Fe-CNP و شاهد وقوع بیشتری از femur طبیعی (NF) و درصد کمتری از نکروز femur انتقالی (FHT) و آسیب‌های کلی femur (تمامی F؛ 05/0P<) ارائه دادند. نکروز سر استخوان ران (FHN) و FHS به طور معنی‌داری توسط تیمارهای جیره‌ای تحت تأثیر قرار نگرفتند (05/0P<). به طور کلی، مکمل Fe-CNP، توسط بهبود سیستم ایمنی، وقوع آسیب‌های BCO در جوجه‌های گوشتی را کاهش می‌دهد.
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