Characteristics Determination of Rheb Gene and Protein in Raini Cashmere Goat

محورهای موضوعی : Camel

م.ر. محمدآبادی ¹ ( Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran )
ف. توحیدی‌ نژاد ² ( Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran )

تاریخ دریافت : 1395/07/20 تاریخ پذیرش : 1395/11/27 تاریخ انتشار : 1396/03/11

کلید واژه: Goat, interaction, base sequence, isoelectric point,

چکیده مقاله :

The aim of the present study was todeterminecharacteristics of Rheb gene and protein in Raini Cashmere goat. Comparative analyses of the nucleotide sequences were performed. Open reading frames (ORFs), theoretical molecular weights of deduced polypeptides, the protein isoelectric point, protein characteristics and three-dimensional structures was predicted using online standard softwares. The full cDNA nucleotide sequence shares 99%, 99%, 99% and 94% identity with Inner Mongolia Cashmere goat, cattle, horse and human, respectively. The deduced Rheb protein of Raini Cashmere goat consist of 184 amino acid residues and its predicted molecular weight was 20478 g/mol for the unmodified protein and the estimated isoelectric point (pI) was 5.59. Results showed that Rheb has a RAS domain starting at the amino acid 4 and ending at the amino acid 170. In conclusion, our data shows the Rheb cDNA is 555 bp in length, including a complete ORF corresponding to a polypeptide of 184 amino acids. Our results in Raini Cashmere goat showed that there are 1 N-glycosylation sites, 3 protein kinase C phosphorylation sites, 2 casein kinase II phosphorylation sites, 4 microbodies C-terminal targeting signals, 2 ATP/GTP binding sites motif A (P-loop) and a prenyl group binding site for predicted Psites of Rheb. Raini Cashmere goat Rheb protein also had an interaction with other predicted proteins. Hence, can suggest that Rheb has probably role in goat cells and must detect in future investigations.

چکیده انگلیسی:

هدف این پژوهش تعیین خصوصیات ژن و پروتئین Rheb در بز کرکی راینی بود. آنالیز مقایسه­ای توالی­های نوکلئوتیدی اجرا شد. چارچوب­های قرائت باز (ORFs)، وزن­های ملکولی پلی­پپتیدها، نقاط ایزوالکتریک، خصوصیات پروتئین و ساختار سه بعدی آن با استفاده از نرم افزارهای استاندارد برخط پیش­بینی شد. توالی نوکلئوتیدی کامل cDNA بزکرکی راینی با بز کرکی مغولی، گاو، اسب و انسان به ترتیب 99 درصد، 99 درصد، 99 درصد و 94 درصد شباهت داشت. پروتئین Rheb بز کرکی راینی شامل 184 آمینواسید بود و وزن ملکولی پیش‌بینی شده برای پروتئین تغییرنیافته آن 20478 گرم/مول به دست آمد و نقطه ایزوالکتریک آن 59/5 برآورد شد. نتایج نشان داد که دامین RAS در آمینواسید 4 شروع می­شود و تا آمینو‌اسید 170 ادامه دارد. به طور کلی، نتایج نشان داد که cDNA ژن Rheb طولی معادل 555 جفت باز دارد و شامل یک چارچوب قرائت باز کامل مربوط به یک پلی­پپتید 184 آمینواسیدی است. نتایج در بز کرکی راینی نشان داد که یک سایت ان-گلیکوسیلاسیون، سه سایت فسفریلاسیون پروتئین کیناز C، دو سایت فسفریلاسیون کازئین کیناز II، چهار میکروبادی C ترمینال هدف گیرنده سیگنال­ها، دو سایت باند شونده به ATP/GTP موتیف A (P-loop) و یک سایت باند شونده به گروه پرنیل برای سایت­های P پیش‌بینی شده برای Rheb وجود دارد. همچنین پروتئین Rheb بز کرکی راینی اثرمتقابلی با پروتئین­های پیش‌بینی شده دیگر داشت. بنابراین، می­توان پیشنهاد کرد که Rheb احتمالاً در سلول­های بز نقش بازی می­کند و این نقش باید در پژوهش­های آینده مورد بررسی قرار گیرد.

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