Genetic Diversity and Molecular Phylogeny of Iranian Sheep Based on Cytochrome b Gene Sequences

محورهای موضوعی : Camel

س. ساور سفلی ¹ (Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran)
ح.ر. سیدآبادی ² (Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran)
ع. جوانروح علی‌آباد ³ (Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran)
ر. سید شریفی ⁴ (Department of Animal Science, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran)

تاریخ دریافت : 1395/07/13 تاریخ پذیرش : 1395/11/12 تاریخ انتشار : 1396/03/11

کلید واژه: genetic diversity, Iranian sheep, phylogenetic analysis, mitochondrial cytochrome b gene,

چکیده مقاله :

Phylogenetic relationships and genetic variation between two Iranian sheep breeds were analyzed using cytochrome b (cyt-b) gene sequences. The genomic DNA was isolated by salting out method and amplified cytochrome b gene using polymerase chain reaction restriction (PCR) method with a pair of primer. A partial sequence of cyt-b gene of Iranian sheep is 780 bp and contained 13 variable sites and 11 haplotypes. Phylogenetic analysis of haplotype in the combination with the sheep from GenBank showed that Iranian sheep made a separated cluster. This study is provided useful information for understanding relationships between breeds from different parts of the world. This study may simplify the future researchers and breeders for better understanding the genetic structure and breed differentiation for designing future breeding strategies to the conservation of animal genetic resources.

چکیده انگلیسی:

روابط فیلوژنتیکی و تنوع ژنتیکی در دو نژاد گوسفند ایرانی بر اساس ژن سیتوکروم b مورد بررسی قرار گرفت. DNA ژنومی با استفاده از روش نمکی استخراج و با استفاده از روش PCR، ژن سیتوکروم b تکثیر شد. بخشی از ژن سیتوکروم b به طول 780 جفت باز، تعیین توالی و 13 ناحیه چند شکل و 11 هاپلوتایپ در نمونه­های مورد مطالعه، مشاهده شد. آنالیز فیلوژنتیکی براساس مقایسه هاپلوتایپ­ها با سایر نژادهای ثبت شده در پایگاه اطلاعاتی، نشان داد که گوسفندان ایرانی در شاخه­ای مجزا قرار دارد. این مطالعه اطلاعات مفیدی در خصوص درک بهتر از روابط بین نژادهای مختلف ارائه می­دهد. همچنین این مطالعه اطلاعات مفیدی جهت درک بهتر ساختار ژنتیکی و تفاوت­های نژادی، برای تببین استراتژی­های اصلاح نژادی و برنامه حفاظت از منابع ژنتیکی دامی در اختیار محققین و اصلاحگرها قرار می­ دهد.

منابع و مأخذ:

Alves P.C., Ferrand N., Suchentrunk F. and Harris D.J. (2003). Ancient introgression of Lepus timidus mtDNA into Lepus granatensis and Lepus europaeus in the Iberian Peninsula. Mol. Phylogenet. Evol. 27, 70-80.

Atashi H. and Izadifar J. (2012). Estimation of individual heterosis for lamb growth in Ghezal and Mehraban sheep. Iranian J. Appl. Anim. Sci. 2, 127-130.

Burgstaller M., Iain G., Johnston L. and Poulton J. (2015). Mitochondrial DNA disease and developmental implications for reproductive strategies Joerg Patrick. Mol. Hum. Reprod. 21, 11-22.

Chen S., Fan B., Liu B., Yu M., Zhao S., Zhu M., Xiong T. and Li K. (2006). Genetic variations of 13 indigenous Chinese goat breeds based on cytochrome b gene sequences. Biochem. Gen. 44, 87-95.

FAO. (1993). Food and Agriculture Organization of the United Nations (FAO), Rome, Italy.

Javanrouh A., Banabazi M.H., Esmaeilkhanian S., Amirinia C., Seyedabadi H.R. and Emrani H. (2006). Optimization on salting out method for DNA extraction from animal and poultry blood cells. Pp. 103 in Proc. 57^th Ann. Meet. European Assoc. Anim. Prod. Antalya. Turkey.

Javanrouh Aliabad A., Khodamoradi S. and Seyedabadi H.R. (2016). Analysis of the genetic diversity and the phylogenetic evolution of Iranian sheep based on D-loop region sequences. J. Vet. Res. 20, 353-361.

Librado P. and Rozas J. (2009). Dnasp V5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 25, 1451-1452.

Mobini B. (2013). A quantitative evaluation of different regions of skin in adult Iranian native sheep. Vet. Med. 58, 260-263.

Molaee V., Osfoori R., Eskandari Nasab M.P. and Qanbari S. (2009). Genetic relationships among six Iranian indigenous sheep populations based on microsatellite analysis. Small rumin. Res. 84(1), 121-124.

Patwardhan A., Ray S. and Roy A. (2014). Molecular markers in phylogenetic studies. A review. J. Phylogen. Evol. Biol. 2, 1-9.

Ruane P., Lang J., DeJesus E., Berger D.S. and Dretler R. (2006). Pilot study of once-daily simplification therapy with abacavir/lamivudine/zidovudine and efavirenz for treatment of HIV-1 infection. HIV Clin. Trials. 7, 229-236.

Sawaimul A.D., Sahare M.G., Ali S.Z., Sirothia A.R. and Kumar S. (2014). Assessment of genetic variability among Indian sheep breeds using mitochondrial DNA cytochrome b region. Vet. World. 7, 852-855.

Sultana S., Mannen H. and Tsuji S. (2003). Mitochondrial DNA diversity of Pakistani goats. J. Anim. Genet. 34, 417-421.

Tamura K. and Nei M. (1993). Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol. Biol. Evol. 10, 512-526.

Tamura K., Stecher G., Peterson D., Filipski A. and Kumar S. (2013). MEGA6: molecular evolutionary genetics analysis version 6.0. Mol. Biol. Evol. 30, 2725-2729.

Tolonea M., Mastrangeloa S., Rosac A.J.M. and Portolanoa B. (2012). Genetic diversity and population structure of Sicilian sheep breeds using microsatellite markers. Small Rumin. Res. 102, 18-25.

Xin W., Yue-Hui M.A. and Hong C. (2006). Analysis of the genetic diversity and the phylogenetic evolution of Chinese sheep based on cyt-b gene sequences. Acta Genet. Sinica. 33, 1081-1086.