Genetic Diversity and Molecular Phylogeny of Iranian Sheep Based on Cytochrome b Gene Sequences
محورهای موضوعی : Camelس. ساور سفلی 1 , ح.ر. سیدآبادی 2 , ع. جوانروح علی‌آباد 3 , ر. سید شریفی 4
1 - Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
2 - Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
3 - Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4 - Department of Animal Science, Faculty of Agricultural and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
کلید واژه: genetic diversity, Iranian sheep, phylogenetic analysis, mitochondrial cytochrome b gene,
چکیده مقاله :
Phylogenetic relationships and genetic variation between two Iranian sheep breeds were analyzed using cytochrome b (cyt-b) gene sequences. The genomic DNA was isolated by salting out method and amplified cytochrome b gene using polymerase chain reaction restriction (PCR) method with a pair of primer. A partial sequence of cyt-b gene of Iranian sheep is 780 bp and contained 13 variable sites and 11 haplotypes. Phylogenetic analysis of haplotype in the combination with the sheep from GenBank showed that Iranian sheep made a separated cluster. This study is provided useful information for understanding relationships between breeds from different parts of the world. This study may simplify the future researchers and breeders for better understanding the genetic structure and breed differentiation for designing future breeding strategies to the conservation of animal genetic resources.
روابط فیلوژنتیکی و تنوع ژنتیکی در دو نژاد گوسفند ایرانی بر اساس ژن سیتوکروم b مورد بررسی قرار گرفت. DNA ژنومی با استفاده از روش نمکی استخراج و با استفاده از روش PCR، ژن سیتوکروم b تکثیر شد. بخشی از ژن سیتوکروم b به طول 780 جفت باز، تعیین توالی و 13 ناحیه چند شکل و 11 هاپلوتایپ در نمونههای مورد مطالعه، مشاهده شد. آنالیز فیلوژنتیکی براساس مقایسه هاپلوتایپها با سایر نژادهای ثبت شده در پایگاه اطلاعاتی، نشان داد که گوسفندان ایرانی در شاخهای مجزا قرار دارد. این مطالعه اطلاعات مفیدی در خصوص درک بهتر از روابط بین نژادهای مختلف ارائه میدهد. همچنین این مطالعه اطلاعات مفیدی جهت درک بهتر ساختار ژنتیکی و تفاوتهای نژادی، برای تببین استراتژیهای اصلاح نژادی و برنامه حفاظت از منابع ژنتیکی دامی در اختیار محققین و اصلاحگرها قرار می دهد.
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