Association of Bovine Follicular Stimulating Hormone (FSHβ) Gene Polymorphism with Reproduction Traits in Iranian Holstein Bulls
محورهای موضوعی : Camel
م.و. قاسمی
1
(Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran)
ا. قربانی
2
(Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran)
کلید واژه: semen quality, follicular stimulating hormone, Iranian Holstein bulls,
چکیده مقاله :
The present study was aimed to examine the association of bovine follicular stimulating hormone gene polymorphism with sperm quality traits including sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh motile sperm in each milt ejaculation (NFMSE), motility before and after the freezing (MBATF) and number of post thaw motile sperm in each milt ejaculation (NPTMSE).We used polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in 83 bulls belonging to two progeny test center. The frequency of the A (PSTI+) and B (PSTI-) alleles were 0.675 and 0.325, respectively. The genotype frequency for AA and AB were 0.35 and 0.65, respectively. The BB genotype was omitted for analysis.Mixed and probity models analyses of sperm quality traits considering genotype and environment as fixed effects and animal as a random effect suggested that sire was a significant source of variation (P<0.001) in all traits.The AB genotype resultedin a significant increase in TS (P<0.0425), NPP (P<0.0302) traits greater than AA genotype.However, AA genotype had significant effect on PTSM (P<0.0001).But not on sperm volume (SV) (P=0.1749), sperm concentration (SPCO) (P=0.1423), fresh sperm motility (FSM) (P=0.5327), number of post thaw motile sperm in each milt ejaculation (NPTMSE) (P=0.5249), total post thaw motile sperm (TPTMS) (P=0.3982), total fresh motile sperm (TFMS) (P=0.2667), total post thaw motile sperm (TPTMS) (P=0.5898) and motility before and after the freezing (MBATF) (P=0.1785). These results indicate that new molecular markers associated with sperm quality traits can be used in marker-assisted selection in bulls.
مطالعه‌ی حاضر با هدف بررسی ارتباط چند شکلی ژن هورمون محرک فولیکولی گاوی با صفات کیفیت اسپرم از جمله حجم اسپرم، جمعیت اسپرم در هر میلی‌متر انزال، کل جمعیت اسپرم در هر انزال، جنبندگی اسپرم تازه، تعداد اسپرم تازه جنبا در هر میلی‌لیتر انزال، کل جمعیت اسپرم تازه متحرک در هر انزال، جنبندگی اسپرم بعد از یخ‌گشایی، تعداد اسپرم تازه جنبا در هر میلی‌لیتر انزال بعد از یخ‌گشایی، کل جمعیت اسپرم زنده بعد از یخ‌گشایی، تعداد پایت تولیدی در هر انزال و نسبت جنبندگی قبل و بعد از انجماد انجام شد. 83 گاو نر متعلق به دو مرکز آزمون نتاج با استفاده از روش (PCR-RFLP) تعیین ژنوتیپ شدند. دو الل A و B و دو ژنوتیپ مشاهده شدند. فراوانی الل A (PSTI+) و الل B (PSTI-) به ترتیب 675/0 و 325/0 به دست آمد. فراوانی ژنوتیپ‌های AA و AB به ترتیب 35/0 و 65/0 بود. ژنوتیپ BB در این بررسی مشاهده نشد. تجزیه و تحلیل مدل مختلط آماری صفات کیفیت اسپرم با توجه به ژنوتیپ و محیط به عنوان اثرات ثابت و حیوان به عنوان یک اثر تصادفی نشان می‌دهد که پدر یک منبع مهم تنوع (001/0P<) در همه صفات بود. ژنوتیپ AB منجر به افزایش قابل توجهی در تعداد کل اسپرم (0425/0P<)، تعداد پایوت تولیدی در هر انزال (0302/0P<) در مقایسه با ژنوتیپ AA شد. لکن، ژنوتیپ AA اثر معنی‌داری بر جنبندگی اسپرم بعد از یخ‌گشایی (0001/0P<) داشت. اما بر روی حجم اسپرم (1749/0P=)، جمعیت اسپرم در هر میلی‌لیتر انزال (1423/0P=)، جنبندگی اسپرم تازه (5327/0P=)، تعداد اسپرم تازه جنبا در هر میلی‌لیتر انزال بعد از یخ‌گشایی (5249/0P=)، کل جمعیت اسپرم زنده بعد از یخ‌گشایی (3982/0P=)، کل جمعیت اسپرم تازه متحرک در هر انزال (2667/0P=)، کل جمعیت اسپرم زنده بعد از یخ‌گشایی (5898/0P=) و نسبت جنبندگی قبل و بعد از انجماد (1785/0P=) تأثیر معنی‌داری نداشت. این نتایج نشان می‌دهد که استفاده از نشانگرهای مولکولی جدید در ارتباط با صفات کیفیت اسپرم می‌تواند در انتخاب به کمک نشانگر در گاو استفاده شود.
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