Antioxidant Effects of Thyme (Thymus vulgaris) Extract on Ram Sperm Quality during Cryopreservation
محورهای موضوعی : Camel
و. واحدی
1
(
Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
)
ن. هدایت ایوریق
2
(
Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
)
م. بهروزلک
3
(
Department of Animal Science, Faculty of Agriculture, Payame Noor University, Tehran, Iran
)
ع. دیرنده
4
(
Department of Animal Science, Faculty of Agriculture, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
)
کلید واژه: Antioxidant, freeze-thawing, ram semen, <, i>, Thymus vulgaris<, /i>, extract,
چکیده مقاله :
Oxidative stress during freezing-thawing reduces sperm motility, viability, membrane functions and antioxidant capacity. Thymus vulgaris has antioxidant properties due to phenolic compounds. The purpose of current study was to evaluate the effect of Thymus vulgaris extract as a natural antioxidant on post-thawed Moghani ram sperm quality. Semen samples were collected twice a week from four rams. Samples were then pooled and diluted with Tris based extender supplemented with different concentrations of Thymus vulgaris (0, 2, 4, 8, 12 and 16 mL per dL diluent solutions). Following the cooling and equilibration stages for semen samples, the samples were frozen and stored in liquid nitrogen until evaluation. Following this freezing procedure, samples were thawed, and the sperm motility and velocity parameters, viability and plasma membrane integrity were evaluated. Our results showed that the addition of 2 and 4 mL/dL extracts at the extender stage increased the total motility percentages (P<0.05). Addition of 4 mL/dL extract improved progressive motility and percentages of viability compared to the control group and high levels of extract groups (P<0.05). Plasma membrane integrity of sperm in 4 and 8 mL/dL extract groups were greater (P<0.05) than the control and 12 and 16 mL/dL extract groups. Also, addition, 16 mL/dL extract had a significantly negative effect on all evaluated traits (P<0.05). In conclusion, supplementation of extender with intermediate amounts of Thymus vulgaris extract improves the quality of ram sperm after freezing and thawing in a dose-dependent manner.
استرس اکسیداتیو طی فرآیند انجماد-یخ‌گشایی اسپرم به وجود آمده و باعث کاهش تحرک، زنده مانی، عملکردهای غشایی، ظرفیت آنتی اکسیدانی و در نهایت باروری سلولهای اسپرم میشود. گیاه آویشن به دلیل داشتن ترکیبات فنولی دارای خاصیت آنتی‌اکسیدانی است. هدف این پژوهش بررسی تأثیر عصاره گیاه آویشن به عنوان یک آنتی‌اکسیدان طبیعی بر کیفیت اسپرم منجمد-یخ‌گشایی شده قوچ مغانی بود. در این مطالعه از 4 رأس قوچ مغانی هفته‌ای دو بار اسپرم‌گیری شد. سپس نمونه‌های منی به طور یکسان با هم مخلوط شدند و با رقیق‌کننده بر پایه تریس که حاوی سطوح مختلف عصاره آویشن (0، 2، 4، 8، 12 و 16 میلی‌لیتر در دسی‌لیتر محلول رقیق‌کننده) بود، رقیق شدند. نمونه‌ها پس از طی مراحل سردسازی و انجماد تا زمان ارزیابی در داخل ازت مایع نگهداری شدند. بعد از یخ‌گشایی، پارامترهای حرکتی اسپرم، زنده‌مانی و تست یکپارچگی غشاء مورد ارزیابی قرار گرفت. نتایج نشان داد که افزودن سطوح 2 و 4 دسی‌لیتر/میلی‌لیتر عصاره درصد تحرک کلی اسپرمها را افزایش داد (05/0P<). همچنین غلظت 4 دسی‌لیتر/میلی‌لیتر عصاره به طور معنی‌داری تحرک پیش‌رونده و درصد زنده‌مانی اسپرمها را نسبت به گروه شاهد و گروههای تیماری حاوی سطوح بالاتر بهبود داد (05/0P<). درصد یکپارچگی غشای پلاسمایی اسپرمها در گروههای 4 و 8 دسی‌لیتر/میلی‌لیتر در مقایسه با گروه شاهد و گروههای 12 و 16 به طور معنی‌داری بالاتر بود (05/0P<). همچنین افزودن 16 دسی‌لیتر/میلی‌لیتر به طور معنی‌داری اثر منفی بر تمامی صفات ارزیابی شده داشت (05/0P<). در نتیجه استفاده از عصاره آویشن در رقیق‌کننده منی منجر به بهبود کیفیت اسپرم منجمد-یخ‌گشایی‌شده قوچ شد که این اثر وابسته به دوز می‌باشد.
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