Ruminal Methane Emission, Microbial Population and Fermentation Characteristics in Sheep as Affected by Malva sylvestris Leaf Extract: in vitro Study
محورهای موضوعی : Camelص. خاموشی 1 , ف. کفیل‌زاده 2 , ح. جهانی-عزیزآبادی 3 , و. ناصری 4
1 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
2 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
4 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
کلید واژه: Kinetics, rumen, partitioning factor, protozoa, cellulolytic bacteria,
چکیده مقاله :
The objective of this study was to investigate in vitro effect of Malva sylvestris leaf extract (at 0, 25, 50 and 100 µL/30 mL of medium) on sheep ruminal cellulolytic and total viable bacteria growth, protozoa populations, methane production, neutral detergent fiber degradability (NDFD) and fermentation efficiency of oat hay. The addition of Malva sylvestris leaf extract at 25, 50 and 100 µL led to a linear increase (P<0.01) in vitro truly degraded dry matter (INTDDM), NDFD and partitioning factor (PF) of oat hay and decrease (P<0.01) methane emission after 24 hours of incubation. The addition of Malva sylvestris leaf extract resulted in a decrease (P<0.01) in potential of gas production at 25 and 50 µL and increase in lag time (0.95, 1.01 and 1.13 h relative to 0.61 h), constant rate of gas production (b)and gas produced at half-life (c)at 25, 50 and 100 µL. The addition of this extract decreased a number of total protozoa and Entodinium, Isotrichae, Diplodinium and Ophryoscolex species (P<0.01). The number of total and cellulolytic bacteria was not influenced by the addition of Malva sylvestris leaf extract. The result of this study demonstrated that Malva sylvestris extract had some potential for improving the rumen fermentation.
هدف از این مطالعه بررسی اثر عصاره برگ گیاه پنیرک (به میزان صفر، 25، 50 و 100 میکرولیتر\30 میلی لیتر محیط کشت) بر رشد باکتریهای سلولایتیک و کل باکتریهای زنده، جمعیت پروتوزواها، تولید متان، پتانسیل تجزیه الیاف نامحلول در شوینده خنثی، و بازده تخمیر شکمبه ای علوفه یولاف در شکمبه گوسفند در شرایط برون تنی بود. افزودن عصاره پنیرک به میزان 25، 50 و 100 میکرولیتر، سبب افزایش خطی(01/0>P) قابلیت هضم حقیقی ماده خشک، الیاف نامحلول در شوینده خنثی و فاکتور تفکیک علوفه یولاف و کاهش (01/0>P) تولید متان بعد از 24 ساعت انکوباسیون در شرایط برون‌تنی، شد. نسبت به تیمار شاهد، افزودن عصاره برگ پنیرک در مقادیر 25 و 50 میکرولیتر سبب کاهش(01/0>P) پتانسیل تولید گاز و در مقادیر 25، 50 و 100 میکرولیتر سبب افزایش (01/0>P) فاز تأخیر (به ترتیب 95/0، 01/1 و 13/1 نسبت به 61/0 ساعت)، ثابت نرخ تولید گاز (b) و ثابت نرخ تولید گاز در زمان تولید 50 درصد از کل گاز تولیدی (c)، شد. افزایش این عصاره تعداد کل پروتوزواها و گونه‌های انتودنیوم‌ها، ایزوتریشا‌ها، دیپلودنیوم و گونه اوفیروس کولکس را کاهش داد (01/0>P). تعداد کل باکتری‌ها و باکتریهای سلولولایتیک تحت تأثیر افزودن عصاره برگ پنیرک قرار نگرفت. نتایج این مطالعه نشان داد که عصاره برگ پنیرک دارای پتانسیل بهبود تخمیر شکمبه‌ای می‌باشد.
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