Use of an in vitro Rumen Gas Production Technique to Evaluate the Nutritive Value of Five Forage to Concentrate Ratios
محورهای موضوعی : Camel
س. اربابی
1
(
Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
)
ت. قورچی
2
(
Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
)
س. رمضانپور
3
(
Department of Animal and Poultry Nutrition, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural Resources, Gorgan, Iran
)
کلید واژه: methane, tannin, faba bean, <, i>, in vitro<, /i>, gas production,
چکیده مقاله :
This study presents the chemical composition and in vitro fermentation of five diets with different forage (alfalfa) to concentrate (faba bean) (F:C) ratios, F0:C100, F25:C75, F50:C50, F75:C25, F100:C0 on a dry matter (DM) basis. Results indicate that the chemical composition in term of organic matter (OM), ether extract (EE), crude protein (CP), acid detergent fiber (ADF), neutral detergent fiber (NDF), neutral detergent insoluble protein (NDIP), acid detergent insoluble protein (ADIP) and total phenols (TPh), tannin (TT) and condensed tannin (TCT) were varies among five diets (P<0.05). After an initial gas test to evaluate 96 h gas production profiles of diets, the time to half maximal gas production was calculated and a second incubation was conducted with fermentation stopped at substrate specific half-time (t1/2) and 24 h for each substrate. In vitro true DM degradability (ivTDDM), OM degradability (ivOMD) were increased (P<0.01) by addition proportions of concentrate in diets. Microbial mass (g/kg DM), metabolizable energy (ME) (MJ/kg DM), were greater in diets which those had been higher degradability. The efficiency of microbial production (PF) (mg/mL) were calculated for both substrate specific t1/2 and 24 h and was not shown differences between experimental diets at 24 h but F100:C0 was lesser (1.17 mg/mL) at substrate specific t1/2 (P<0.01). Gas produced from fermentable fraction (B) and the rate of gas production (c) were (P<0.01) greater in diets with grater concentrate ratio. Increasing the F:C ratio increased ruminal pH and N ammonia and affected concentrations of short-chain fatty acid (SCFA) (P<0.01). Amount of CH4 emission from 13 to 17.16 g/kg DM and the great value was related to F100:C0.
این پژوهش به منظور بررسی ترکیبات شیمیایی و تخمیر برونتنی پنج جیره با نسبتهای مختلف علوفه (یونجه) به کنسانتره (باقلا)، علوفه(صفر): کنسانتره (100)، علوفه 25): کنسانتره (75)، علوفه (50): کنسانتره (50)، علوفه (75): کنسانتره (25)، علوفه (100): کنسانتره (صفر) براساس ماده خشک انجام شد. نتایج نشان داد که ترکیبات شیمیایی مانند ماده آلی، چربی خام، پروتئین خام، دیواره سلولی، دیواره سلولی فاقد همیسلولز، پروتئین نامحلول در شوینده خنثی، پروتئین نامحلول در شوینده اسیدی، فنل کل، تانن و تانن متراکم در پنج جیره متفاوت بودند (05/0>P). پس از اولین انکوباسیون جیرههای آزمایشی به مدت 96 ساعت، نیمه عمر تولید گاز محاسبه و دومین و سومین انکوباسیون در زمان t1/4 و 24 ساعت متوقف گردید. قابلیت هضم حقیقی برونتنی ماده خشک و قابلیت هضم حقیقی ماده آلی با افزایش نسبت کنسانتره افزایش یافت (01/0>P). پروتین میکروبی (گرم/کیلوگرم ماده خشک) و انرژی قابل متابولیسم (مگاژول/کیلوگرم ماده خشک) در جیره‌هایی که با تجزیهپذیری بالاتر، بیشتر بود. راندمان تولید پروتئین میکروبی (PF) (میلیگرم/میلیلیتر) در جیرههای آزمایشی مختلف در هر زمان t1/4 و 24 ساعت تفاوت معنیداری نداشت و تنها جیره حاوی 100 درصد علوفه در زمان t1/4 دارای PF پایینتری (17/1 میلیگرم در میلیلیتر) بود. تولید گاز از بخش دارای پتانسیل تخمیر(B) و نرخ تولید گاز (c) در جیرههای دارای مواد متراکم بیشتر، به طور معنیداری بالاتر بود (01/0>P). افزایش نسبت کنسانتره جیره منجر به کاهش pH و نیتروژن آمونیاکی مایع شکمبه شد و غلظت اسیدهای چرب را متأثر ساخت (01/0>P). میزان رهایش گاز متان در جیرههای آزمایشی از 13 تا 16/17 گرم/کیلوگرم ماده خشک متغیر بود و بیشترین مقدار مربوط به جیره حاوی 100 درصد علوفه بود.
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