Treatment of Repeat Breeding of Iraqi Buffaloes Using Different CIDR Protocols
محورهای موضوعی : CamelO.I. Azawi 1 , M. Delpli Ali 2 , O.S. Ahmed 3 , A.S. Al‐Hadad 4 , M.S. Jamil 5 , Hussien Hussien 6
1 - Department of Surgery and Theriogenology, Collegeof Veterinary Medicine, Universityof Mosul, Mosul, Iraq
2 - Ministry of Agriculture, Animal Resources Service General Company, Baghdad, Iraq
3 - Ministry of Agriculture, Animal Resources Service General Company, Animal Research Centre, BuffaloResearch Unit, Mosul, Iraq
4 - Ministry of Agriculture, Animal Resources Service General Company, Baghdad, Iraq
5 - Ministry of Agriculture, Animal Resources Service General Company, Baghdad, Iraq
6 - Ministry of Agriculture, Animal Resources Service General Company, Animal Research Centre, BuffaloResearch Unit, Mosul, Iraq
کلید واژه: GnRH, Buffalo, CIDR, PGF2α, repeat breeding,
چکیده مقاله :
The present study was undertaken to examine the efficacy of a treatment regime using a controlled internal drug release (CIDR) device in conjunction with injections of gonadotropin releasing hormone (GnRH) and prostaglandin F2α (PGF2α) on conception rate of repeat-breeding Iraqi buffaloes. Data were collected from 100 buffaloeswith repeat breeding in Ninevehprovince housed at six private dairy farms. All buffalo cows were characterized as repeat breeding of more than three natural services with regular oestrouscycles without any signs of pregnancy. Any buffalo with abnormal vaginal discharge and >12% of polymorphonuclear cells in uterine smears was excluded from this study. Buffaloes were randomly assigned to four treatment groups of 25 cows each. Buffaloes in treatment 1 were injected with estradiol benzoate 1 mg and GnRH 250 μg on day 0, aCIDR in the anterior vagina from day 0 to 8, on injection with 25 mg of PGF2α on day 7 and GnRH (250 μg) on day 9. Buffaloes in treatment 2 received GnRH (250 μg) on day 0, aCIDR, from day 0 to 7 and 25 mg of PGF2α on day 7. Buffaloes in treatment 3 received a CIDR from day 0 to 7 and 25 mg of PGF2α on day 7. Treatment 4, acontrol group received no treatments. All buffaloes were mated during oestrus and pregnancy status was diagnosed by palpation of uterine contents at day 45. Pregnancy rates in treatments 1 to 4 were 44%, 52%, 24% and 8%, respectively. On average, Buffaloes treated with different CIDR protocols had higher pregnancy rate than control group. It could be concluded that the use of different CIDR protocols can be applied to improve fertility of repeat breeder Iraqi buffaloes.
Drost M. (2007). Bubaline versus bovine reproduction. Theriogenology. 68, 447-449.
Dubuc J., Duffield T.F., Leslie K.E., Walton J.S. and LeBlanc S.J. (2010). Definitions and diagnosis of postpartum endometritis in dairy cows. J. Dairy Sci. 93, 5225-5233.
Fabre-Nys C. and Martin G.B. (1991). Roles of progesterone and oestradiol in determining the temporal sequence and quantitative expression of sexual receptivity and preovulatory LH surge in the ewe. Endocrinology. 130, 367-379.
Jaunadine M.R. (1986). Reproduction in water buffalo. Pp. 443-449 inCurrent Therapy in Theriogenology. D.A. Morrow, Ed., W.B. Saunders, Philadelphia, PA.
Jiang R.M., Wei Y.M. and Ling Z.J. (2003). The estrous and conception rates in swamp buffaloes after synchronization by PGc CIDR alone or cimbined with other hormones. Chinese J. Anim. Sci. 39, 17-18.
Justolin P., Morelli P., Reis M., Sá Filho O., AragonF., Veras M., Soriano S. and Vasconcelos J.L. (2010). Effects of treatments with hCG or GnRH on serum progesterone (P4) and conception rates (CR) in lactating dairy cows submitted to timed artificial insemination (AI) or embryo transfer (ET). J. Dairy Sci. 93, 675-683.
MacMillan K.L. and Peterson A.J. (1993). A new intravaginal progesterone releasing device for cattle (CIDR-B) for oestrous synchronization increasing pregnancy rates and the treatment of post-partum anestrus. Anim. Reprod. Sci. 33, 1-25.
Metwelly K.K. (2001). Postpartum anestrus in buffalo cows: causes and treatment. Pp. 259-267 inProc. 6th Scientific Congress Egyptian Society for Cattle Diseases, AssuitUniversity, Assiut, Egypt.
Metwelly K.K. (2006). Treatment of ovarian inactivity in postpartum buffalo-cows with special reference to its economic evaluation. Assiut Vet. Med. J. 52, 214-225.
Randel R.D., Lammoglia M.A., Lewis A.W., Neuendorff D.A. and Guthrie M.J. (1996). Exogenous PGF2α enhanced GnRH-induced LH release in postpartum cows. Theriogenology. 45, 643-654.
Ravikumar K., Asokan S.A., Veerapandian C. and Palanisamy A. (2010). Ovarian status serum progesterone (P4) level and conception rate in OVSYNCH plus CIDR treated postpartum buffaloes. Tamilnadu J. Vet. Anim. Sci. 7, 1-5.
Ryan D.P., Snıjders S., Condon T., Grealy M., Sreenan J. and O’farrell K.J. (1994). Endocrine and ovarian responses and pregnancy rates in dairy cows following the administration of a gonadotrophin releasing hormone analog at the time of artificial insemination or at mid-cycle postinsemination. Anim. Reprod. Sci. 34, 179-191.
Senger P.L. (1994). The estrus detection problem: new concepts, technologies, and possibilities. J. Dairy Sci. 77, 2745-2753.
Singh C. (2003). Response of anestrus rural buffaloes (Bubalus bubalis) to intravaginal progesterone implant and PGF2α injection in summer. J. Vet. Sci. 4, 137-141.
Singh J., Alanda A.S. and Adams G.P. (2000). The reproductive pattern and efficiency of female buffaloes. Anim. Reprod. Sci. 61, 593-604.
Singh S., Wani N.A. and Maurya S.N. (2003). Use of different hormones for the treatment of postpartum anestrous in buffaloes under field condition. Indian J. Anim. Sci. 73, 894-896.
Steckler T.L., Faulkner D.B., Nash T.G., Dahlquist J.M., IrelandF.A. and Kesler D.J. (2002). Improvement of pregnancy and rescue of embryos in beef cows. Theriogenology. 57, 561-567.
Suthar V.S. and Dhami A.J. (2010). Estrus detection methods in buffalo. Vet. World. 3, 94-96.
Zaabel S.M., Hegab A.O., Montasser A.E. and El-Sheikh H. (2009). Reproductive performance of anestrous buffaloes treated with CIDR. Anim. Reprod. 6, 460-464.
Zerbe H., Gregory C. and Grunert E. (1999). Zur behandlung ovariell bedingter zyklusstorungen beim milchrind mit progesteron-abgebenden vorrichtungen. Tierarztl Umsch. 54, 189-192.