Administration of Different Levels of Arginine and Lysine Coupled with Copper for Change the Copper Concentration of Milk in the Lactating Zandi’s Ewes
محورهای موضوعی : Camel
ا. پردل
1
(Department of Animal Physiology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran)
ه. خزعلی
2
(Department of Animal Physiology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran)
ح. رکنی
3
(Department of Education, Institute of Applied Scientific Education of Jihad-e-Agriculture, Tehran, Iran)
ع. حسینی
4
(Department of Animal Physiology, Faculty of Life Science and Biotechnology, Shahid Beheshti University, Tehran, Iran)
کلید واژه: Copper, Lysine, arginine, ewe milk, lactation stage,
چکیده مقاله :
Copper (Cu) plays an essential role in the human and animal body system. One of the most important sources of Cu is milk. The purpose of this study was to determine the effect of different doses of injection of inorganic Cu and organic Cu as Cu-arginine and Cu-lysine on Cu concentrations of milk in the lactating Zandi’s ewes. This experimental research involved 54 lactating Zandi’s ewes collected from the department of State Organization Agriculture of Tehran province, Iran. They were allocated into three main groups included: group 1) which received inorganic form of Cu as copper chloride, groups 2 and 3) which received organic Cu as Cu-arginine or Cu-lysine (25, 50 and 100 mg kg-1 of body weight (BW)) in the chelate form, respectively. The milk samples were taken 30 min before and 6 hours after intravenous injection of Cu preparations. The Cu concentration in milk was measured using the flame atomic absorption spectrometric method. There was no significant difference among the groups in milk’s Cu concentration before administration of either inorganic or organic Cu. Data analysis showed that the administration of inorganic Cu caused a significant increase (P<0.01) of Cu concentration in the milk in a dose dependent manner. Furthermore, the administration of Cu-arginine or Cu-lysine in chelate form caused a significant increase (P<0.01) in milk Cu concentration compared to the inorganic Cu. In conclusion, administration of different forms of Cu influenced the Cu concentration in the milk. The organic forms of Cu led to a significant increase in milk Cu concentration in a dose dependent manner compared to the inorganic form of Cu.
مس نقش اساسی در سیستم بدن انسان و حیوان دارد. یکی از مهمترین منابع مس، شیر است. هدف از این مطالعه مشخص ساختن این بود که تزریق دوزهای متفاوت مس غیرآلی و مس آلی به صورت کلاته با آمینواسیدهای آرژنین و لیزین می‌تواند غلظت مس را در شیر بزهای زندی شیرده تغیر دهد. این پژوهش تجربی شامل 54 میش زندی شیرده که از سازمان کشاورزی استان تهران جمع‌آوری شده، در 3 گروه اصلی به شرح زیر اختصاص داده شد: گروهی که کلرید مس را در شکل معدنی (25، 50 و 100 میلی‌گرم بر کیلوگرم، وزن بدن) دریافت کردند و گروه‌هایی که مس آلی در دوزهای مختلف آرژنین و یا لیزین (25، 50 و 100 میلی‌گرم بر کیلوگرم، وزن بدن) به صورت کلاته شده دریافت کردند. نمونه‌های شیر 30 دقیقه قبل و 6 ساعت پس از تزریق داخل وریدی آماده‌های مس گرفته شد. غلظت مس در شیر با استفاده از روش طیف‌سنجی جذب اتمی شعله اندازه‌گیری شد. قبل از تزریق مس معدنی و یا آلی در غلظت مس شیر تفاوت معنی‌داری بین دو گروه وجود نداشت. تجزیه و تحلیل داده‌ها نشان داد که تجویز مس غیر آلی باعث افزایش قابل توجهی از غلظت مس در شیر به صورت وابسته به دوز شد (01/0<P). همچنین، تزریق آرژنین و یا لیزین با مس در فرم کلاته باعث افزایش قابل توجهی در غلظت مس شیر در مقایسه با غیر آلی مس در صورت وابسته به دوز شد (01/0<P). با توجه به نتایج بدست آمده، مصرف فرم‌های مختلف مس غلظت مس در شیر را تحت تأثیر قرار می‌دهد.
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