Effect of Tempol in Sasaki Diluent on the Quality and Fertility of Cryopreserved Chicken Semen
محورهای موضوعی : Camel
M. Shanmugam
1
(ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad-30, India)
R.K. Mahapatra
2
(ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad-30, India)
کلید واژه: Lipid peroxidation, Dimethyl sulfoxide, acrosome reaction, PD-1 Chicken,
چکیده مقاله :
Sperm are exposed to oxidative stress during cryopreservation leading to damage of membrane lipids. In the present study, the effects of adding Tempol during cryopreservation of chicken semen on post-thaw semen parameters and fertility were investigated. Adult PD-1linesemen were cryopreserved using 4% dimethyl sulfoxide (DMSO) in Sasaki diluent (SD). Tempol (1 and 5 mM) was added to the semen-cryopreservation mixture at final concentrations. The semen with additives was filled in 0.5 mL French straws and exposed to liquid nitrogen vapours for 30 min and then stored in liquid nitrogen. The semen straw was thawed at 5 ˚C for 100 seconds and evaluated for sperm motility, live, abnormal, and acrosome intact sperm. The seminal plasma was evaluated for lipid peroxidation. The fertilization potential of the cryopreserved sperm was evaluated after insemination in the PD-1line hens. Sperm parameters after thawing were significantly (P<0.05) lower in the cryopreserved groups compared to fresh semen. Lipid peroxidation was significantly (P<0.05) higher in cryopreserved groups. Fertility was significantly (P<0.05) lower in all the cryopreserved groups. In conclusion, the addition of template cryo diluent did not improve the post-thaw semen parameters or fertility.
Azadi L., Tavalaee M., Deemeh M.R., Arbabian M. and Nasr-Esfahani M.H. (2017). Effects of tempol and quercetin on human sperm function after cryopreservation. Cryo Lett. 38, 29-36.
Bateni Z., Azadi L., Tavalaee M., Kiani-Esfahani A., Fazilati M. and Nasr-Esfahani M.H. (2014). Addition of tempol in semen cryopreservation medium improves the post-thaw sperm function. Syst. Biol. Reprod. Med. 60, 245-250.
Burrows W.H. and Quinn J.P. (1937). The collection of spermatozoa from the domestic fowl and turkey. Poult. Sci. 16, 19-24.
Campbell R.G., Hancock J.L. and Rothschild L. (1953). Counting live and dead bull spermatozoa. J. Exp. Biol. 30, 44-49.
Donoghue A.M. and Donoghue D.J. (1997). Effects of water- and lipid-soluble antioxidants on turkey sperm viability, membrane integrity, and motility during liquid storage. Poult. Sci. 76, 1440-1445.
Hsieh Y.Y., Chang C.C. and Lin C.S. (2006). Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int. J. Biol. Sci. 2, 23-29.
Long J.A. (2006). Avian semen cryopreservation: What are the biological challenges? Poult. Sci. 85, 232-236.
Mara L., Accardo C., Pilichi S., Dattena M., Chessa F., Chessa B., Branca A. and Cappai P. (2005). Benefits of TEMPOL on ram semen motility and in vitro fertility: A preliminary study. Theriogenology. 63, 2243-2253.
Partyka A., Łukaszewicz E. and Niżański W. (2012). Effect of cryopreservation on sperm parameters, lipid peroxidation and antioxidant enzymes activity in fowl semen. Theriogenology. 77, 1497-1504.
Partyka A., Niżański W., Bajzert J., Łukaszewicz E. and Ochota M. (2013). The effect of cysteine and superoxide dismutase on the quality of post-thawed chicken sperm. Cryobiology. 67, 132-136.
Pope C.E., Zhang Y.Z. and Dresser B.L. (1991). A simple staining method for evaluating acrosomal status of cat spermatozoa. J. Zoo Wildl. Med. 22, 87-95.
Pranay Kumar K., Swathi B. and Shanmugam M. (2019). Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken. British. Poult. Sci. 60, 1-6.
Samuni A., Krishna C.M., Mitchell J.B., Collins C.R. and Russo A. (1990). Superoxide reaction with nitroxides. Free Radic. Res. Commun. 9, 241-249.
Santiani A., Acosta A.S., Evangelista S., Vargas S.E., Valdivia M., Cuya M.V., Risopatrón J., González J.R., Sánchez R. and Gutiérrez R.S. (2013). Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation. Theriogenology. 79, 842-846.
SAS Institute. (2003). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Sasaki K., Tatsumi T., Tsutsui M., Niinomi T., Imai T., Naito M., Tajima A. and Nishi Y. (2010). A method for cryopreserving semen from Yakido roosters using N-methylacetamide as a cryoprotective agent. J. Poult. Sci. 47, 297-301.
Surai P.F., Fujihara N., Speake B.K., Brillard J.P., Wishart G.J. and Sparks N.H.C. (2001). Polyunsaturated fatty acids, lipid peroxidation and antioxidant protection in avian semen-Review. Asian-Australasian J. Anim. Sci. 14, 1024-1050.
