Study of Dopamine Receptor Gene Polymorphisms and Their Association with Growth and Egg Production Traits in West Azerbaijan Native Chicken
محورهای موضوعی : Camelم. غلامی 1 , م. غفاری 2 , ع. هاشمی 3
1 - Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
2 - Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
3 - Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
کلید واژه: body weight, native chicken, SNP, Dopamine, number of eggs,
چکیده مقاله :
The objective of this study was to search for single nucleotide polymorphism (SNP)-type polymorphisms in the dopamine D1 receptor in West Azerbaijani native chicken and look for their association with egg production and body weight traits of chickens by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). For this purpose 180 blood samples were taken from native chickens in a poultry breeding station in West Azerbaijan, Iran. DNA was extracted from blood samples using the salting-out procedure. Specific primers were designed for PCR amplification of the 283 base segment of the dopamine D1 receptor gene. In order to demonstration of gene polymorphism, Single-stranded conformation polymorphism followed by sequencing was performed. Four samples from each banding pattern were sent for sequencing. The polymorphism was found in the D1 receptor gene (G123A). We detected three genotypes in DRD1 gene. The frequencies of three genotypes were 0.42 (AA), 0.49 (AG), and 0.09 (GG), respectively. The analysis of variance was performed using of SAS software. The Result shows that there was a significant relationship between genotypes of dopamine D1 receptor gene with egg production traits. This finding indicates that the dopamine D1 receptor gene was polymorphic and could be a candidate locus or linked to a major gene that influences reproductive traits in chickens.
هدف از تحقیق حاضر مطالعه چند شکلی تک نوکلئوتیدی در ژن DRD1 و ارتباط آن با صفات رشد و تولید تخم مرغ در جمعیت مرغان بومی آذربایجان غربی با روش PCR-SSCP بود. برای این منظور نمونههای خون از 180 قطعه مرغ بومی واقع در ایستگاه اصلاح نژاد مرغ بومی آذربایجان غربی گرفته شد. استخراج DNA به روش بهینه یافته نمکی صورت گرفت. قطعه 283 جفت بازی ژن DRD1با استفاده از واکنش زنجیرهای پلیمراز تکثیر گردید. روش تفاوت فرم فضایی رشتههای منفرد (SSCP) جهت شناسایی چند شکلی در نمونهها بکار برده شد. برای شناسایی جهش تک نوکلئوتیدی از روش توالییابی استفاده گردید. برای این منظور 4 نمونه از هر الگوی باندی متفاوت برای توالییابی ارسال گردید که در نتیجه آن سه نوع ژنوتیپ AA، AG و GG به ترتیب با فراوانی 42/0، 49/0 و 09/0 مشاهده شدند. از رویه GLM نرم افزار SAS برای آنالیز دادهها استفاده گردید. نتایج این تحقیق نشان داد که ناحیه مورد بررسی این ژن چند شکل میباشد، بین ژنوتیپ‌های مشاهده شده و صفات تولید تخم مرغ ارتباط معنی‌داری وجود دارد. بنابراین این ژن میتواند به عنوان جایگاه ژنی کاندیدا و یا پیوسته با ژن بزرگ اثر مرتبط با صفات تولید مثلی در مرغ بومی باشد.
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