Effects of Coenzyme Q10 Supplementation on Growth Performance, some Hematological Parameters, Plasma Enzymes Activities in Broilers with Pulmonary Hypertension Syndrome (PHS)
محورهای موضوعی : Camel
1 - Department of Animal Science, Facultyof Agriculture, Payame Noor University, Tehran, Iran-
کلید واژه: blood parameters, broiler chicks, Coenzyme Q10, PHS, plasma enzymes activity,
چکیده مقاله :
This study investigated the effects of coenzyme Q10 (CoQ10) supplementation on hematological, enzymes activities and biochemical parameters in broilers with pulmonary hypertension syndrome (PHS).Two hundred and forty 1-d-old Ross 308 male broiler chicks were randomly allocated into 3 groups with 4 replicates. From d 14, the diets were supplemented with CoQ10 at levels of 0, 20 and 40 mg/kg, respectively, while exposing them to low ambient temperature (10 to 15 ˚C) to induce PHSuntil d 42.Mortality was inspected to determine cause of death and diagnose of PHS. Hematological, biochemical and pathological tests were used to determine the incidence of PHS: total red blood cell (RBC), hemoglobin (HGB), hematocrit (HCT), plasma protein and glucose, activity of alanine transaminase (ALT), aspartate transaminase (AST) and lactate dehydrogenase (LDH).Blood samples were taken at d 21 and 42. At the end of the experiment (wk 6), 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed; the right ventricle was dissected away from the left ventricle and septum and the ratio of right ventricle weight to total ventricle weight (RV/TV) was calculated.Average BW gain and average feed intake were measured weekly from d 15 and average feed conversion ratio was calculated and reported weekly.The results showed that 40 mg/kg CoQ10 supplementationimprovedfeed conversion ratio(FCR)(P<0.05). Moreover, RBC count and plasma protein and glucose level were significantly decreased by 40 mg/kg CoQ10 compared to the other groups, but no significant changes were observed in HGB and HCT. The LDH activity decreased by CoQ10 supplementation (P<0.05). Measurement of malondialdehyde (MDA) content in plasma, RV / TV and mortality due to PHS decreased by 40 mg/kg CoQ10 supplementation (P<0.05). In conclusion, CoQ10 exerted preventive roles in PHS, possible through delivering antioxidant effect of cardiac myocytes and erythrocytes.
مطالعه حاضر برای بررسی اثرات مکملسازی کوآنزیم کیو 10 بر فعالیت آنزیم و فراسنجههای خونی در جوجههای گوشتی در‌گیر با سندرم آسیت، انجام شد. 240 جوجه گوشتی نر سویه راس 308 به طور کاملاً تصادفی به 3 تیمار و برای هر تیمار 4 تکرار اختصاص یافتند. از روز 14 آزمایش، مقادیر0، 20 و 40 میلیگرم به کیلوگرم خوراک پایه اضافه شد. همزمان هم با اعمال تیمارها، جهت القای آسیت، دمای سالن پرورشی به 15-10 درجه سانتیگراد کاهش یافت و این دما تا روز 42 حفظ گردید. تلفات نیر برای تشخیص دلیل مرگ و تعیین تلفات آسیتی به طور روزانه بررسی شدند. آزمایشات خونی، بیوشیمایی و پاتولوژیکی برای تشخص آسیت عبارت بودند از کل گلبول‌های قرمز (RBC)، هموگلوبین (HGB)، هماتوکریت (HCT)، پروتئین و گلوکز خون، فعالیت آنزیمهای آلانین آمینوترانسفراز (ALT)، آسپارتات آمینوترانسفراز (AST) و لاکتات دهیدروژناز (LDH). نمونه برداری از خون در روزهای 21 و 42 انجام شد. در پایان آزمایش، از هر قفس، 2 جوجه، به طور تصادفی انتخاب شده و بعد از کشتار، قبل آنها برداشته شد و بطن راست و بطن چپ از ناحیه سپتوم، جدا گشته و نسبت بطن راست به کل بطن‌ها (RV/TV)، نیز محاسبه گشت. میانگین خوراک مصرفی، افزایش وزن حاصله و ضریب تبدیل غذایی نیز به طور هفتگی، از روز 15، اندازهگیری شدند. نتایج نشان داد که، مکملسازی 40 میلیگرم کوآنزیمکیو 10، سبب بهبود ضریب تبدیل غذایی شد (05/0P<). علاوه بر این، مکملسازی 40 میلیگرم کوآنزیم کیو 10 در پرندگان سبب کاهش معنیدار گلبول قرمز، گلوکز و پروتئین خون و هم چنین فعالیت آنزیم لاکتات دهیدروژناز پلاسما گشت. بین هموگلوبین و هماتوکریت خون پرندگان تیمارهای مختلف اختلاف معنیداری مشاهده نشد. همچنین، سطح 40 میلیگرم کوآنزیم کیو 10 سبب کاهش معنیدار مالون دیآلدئید (MDA)، تلفات آسیتی و شاخص نسبت بطن راست به کل بطن شد. نتیجهگیری اینکه کوآنزیم کیو 10 میتواند با اثرات آنتی اکسیدانی سبب ایجاد محافظت از سلول‌های قلبی و گلبول قرمز شده واز تلفات آسیتی در جوجههای گوشتی جلوگیری نماید.
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