Genetic Variability in Growth Hormone Gene and Association between Restriction Fragment Length Polymorphisms (RFLP) Patterns and Quantitative Variation of Live Weight, Carcass, Behaviour, Heterophil and Lymphocyte Traits in Japanese Quails
محورهای موضوعی : Camelا. نصیری‌فر 1 , م. طالبی 2 , ع. اسمعیلی زاده 3 , ن. عسکری 4 , س.س. سهرابی 5 , ح. مرادیان 6
1 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - استاد گروه علوم دامی، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران
4 - National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
5 - Institute Science and High Technology and Environmental Science, Graduate University of Advanced Technology, Kerman, Iran
6 - Institute Science and High Technology and Environmental Science, Graduate University of Advanced Technology, Kerman, Iran
کلید واژه: Japanese quail, heterozygosity, Growth hormone, Shannon's information index,
چکیده مقاله :
Growth hormone gene plays a critical role in regulating growth and metabolism which leads to potential correlations between the polymorphisms of this gene and economic trait. A 776 bp fragment within the intron 1 region of the growth hormone gene from 346 individuals of an F2 population of Japanese quail was amplified. The polymerase chain reaction (PCR) product was digested using MspI restriction enzyme. restriction fragment length polymorphisms (RFLP) analysis revealed three restriction sites, which led to four different restriction fragments. Four distinctive alleles (A, B, C, and D) and four different genotypes (AA, AB, AC, and AD) were identified in this population. The results indicated that the population deviated from the Hardy-Weinberg equilibrium (P<0.005). The observed heterozygosity and Shannon's information indexes were 0.720 and 0.560, respectively, which demonstrated high diversity in this population. There was a significant association between RFLP patterns and live weight at four and five weeks of age, carcass weight, breast weight and proportion of the internal organs. Carcass weight of the AB birds was less than that of other genotypes (P<0.05). These results suggested that growth hormone gene can be used as a candidate gene for marker-assisted selection to improve performance in Japanese quails.
ژن هورمون رشد نقش اساسی در تنظیم رشد و متابولیسم دارد و منجر به همبستگی‌های بالقوه بین چند‌شکلی این ژن و صفات اقتصادی می‌شود. یک قطعه 776 جفت بازی درون ناحیه اینترون یک ژن هورمون رشد از 346 بلدرچین ژاپنی از جمعیت F2 تکثیر شد. محصول واکنش زنجیره‌ای پلیمراز با استفاده از آنزیم محدود کننده MspI مورد هضم آنزیمی قرار گرفت. آنالیز چند‌شکلی طول قطعه محدود (RFLP) سه جایگاه محدود که منجر به چهار قطعه محدود متفاوت می‌شود را نشان می‌دهد. چهار آلل مشخص (A، B، C و D) و چهار ژنوتیپ متفاوت (AA، AB، AC و AD) در این جمعیت شناسایی شدند. نتایج نشان دادند که جمعیت از تعادل هاردی واینبرگ انحراف دارد (05/0>P). هتروزیگوسیتی و شاخص اطلاعات شانون بدست آمده به ترتیب 720/0 و 560/0 بودند که نشان دهنده تنوع زیاد در این جمعیت بود. ارتباط معنی‌داری بین الگوی های (RFLP) و وزن زنده در چهار و پنج هفتگی، وزن لاشه، وزن سینه و نسبت ارگان‌های داخلی وجود داشت. وزن لاشه پرندگان AB کمتر از ژنوتیپپ‌های دیگر بود (05/0>P). این نتایج پیشنهاد می‌کند که ژن هورمون رشد می‌تواند به عنوان یک ژن کاندید برای انتخاب به کمک نشانگر برای بهبود عملکرد در بلدرچین ژاپنی استفاده شود.
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