Effects of Dietary Supplement of Organic Acids Induced Protective Immunity against Coccidiosis

محورهای موضوعی : Camel

آ.ی. عبدوالهی ¹ , ایکس.جی. یو ² , ی.کیو. فو ³ , م.و. وانگ ⁴ , ن.اس. کی ⁵ , م.اچ. گیزا ⁶ , اس. کالون ⁷ , و.د. پان ⁸ , ایکس.ل. شی ⁹ , ی. فانگ ¹⁰ , اس. ویو ¹¹ , و. هو ¹² , ل.پی. تان ¹³ , کا.ایکس. لی ¹⁴ , ز. وانگ ¹⁵ , جی.جی. زو ¹⁶ , د.ی. فنگ ¹⁷ , جی.کیو. لی ¹⁸

1 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China|Department of Animal Science, Kano University of Science and Technology Wudil, PMB3244, Kano, Nigeria
2 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
3 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
4 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
5 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
6 - College of Animal Science, South China Agricultural University, Guangzhou, China
7 - Department of Animal Science, Njala University, Freetown, Sierra Leone|College of Animal Science, South China Agricultural University, Guangzhou, China
8 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
9 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
10 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
11 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
12 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
13 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
14 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
15 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China
16 - College of Animal Science, South China Agricultural University, Guangzhou, China
17 - College of Animal Science, South China Agricultural University, Guangzhou, China
18 - College of Veterinary Medicine, South China Agricultural University, Guangzhou, China

تاریخ دریافت : 1397/11/12 تاریخ پذیرش : 1398/02/25 تاریخ انتشار : 1398/12/11

کلید واژه: immunity, cytokines, organic acid, Coccidiosis, T-cells,

چکیده مقاله :

This study evaluated effects of three products of dietary organic acids (Acidomix® AFG; Activate® AD and Lacplus) on protective immunity against coccidiosis. Two hundred and eighty 1-day broiler chickens were randomly divided into 8 groups by 5 replications (7 birds per replicate), comprising 2 controls (negative=N; positive=P) and 6 supplemented organic acid groups (1% AFG, 1% DA, 1% Lacplus; 2% AFG, 2% DA and 2% Lacplus). With the exception of N control (uninfected), all 7 groups were orally challenged with 8 × 105 sporulated oocysts of Eimeria tenella at day 12 post-hatch. Samples of blood, spleen and ceca were collected on day 22 and 35 post-hatch. Body weight gain, fecal oocyst shedding, lesion score, mortality rate and pH of the ceca were assessed. ELISA was used to detect Cluster of T cell differentiation (CD4+, CD8+) molecules and reverse transcription polymerase chain reaction (RT-PCR) was employed for detection of pro-inflammatory chemokine (IFNγ-) and cytokines expression (IL-8, IL-15 and IL-17). The result revealed that supplementation of organic acids significantly increased body weight gain, improved feed conversion ratio (FCR), reduced lesion scores and oocyst index (p <0.05) compared to P control (infected non-supplemented). The mortality rate was higher in P control. The pH of ceca was significant (p <0.05) and maximum was observed in P control. Chicks fed 2% DA and 1% Lacplus had greater percentages of CD4 T-cell molecules, but had decreased CD8 T cell molecules, suggesting a protective function of these T-cell subsets in innate immune response against E. tenella infection. The cecal and splenic chemokine and cytokines mRNA expression encoding IFN-γ, IL-8, IL-15 and IL-17 showed higher levels of transcript compared to N and P controls, indicating the organic acid products might have exerted their protective effects by improving their production. It is concluded that the Activate® DA at 2% and Lacplus 1% levels at both 22 and 35-days post-hatch have shown best anticoccidial effects.

چکیده انگلیسی:

این مطالعه آثار سه محصول از اسیدهای ارگانیک جیره‌ای (Acidomix® AFG؛ Activate® AD و Lacplus) روی ایمنی محافظتی بر علیه کوکسیدیوزها را ارزیابی کرد. 280 جوجه 1 روزه گوشتی به ‌طور تصادفی درون 8 گروه شامل 5 تکرار (7 پرنده به ازای هر تکرار)، شامل 2 کنترل (منفی=N؛ مثبت=P) و 6 گروه مکمل اسید ارگانیک (1 درصد AFG، 1 درصد DA، 1 درصد Lacplus، 2 درصد AFG، 2 درصد DA و 2 درصد Lacplus) تقسیم شدند. به استثنای کنترل N (آلوده نشده) ، تمامی 7 گروه به ‌طور خوراکی با 105 × 8 از اووسیت‌های هاگ‌آور شده Eimeria tenella در روز 12 بعد از هچ به چالش کشیده شدند. نمونه‌های خون، طحال و روده کور در روز 22 و 35 بعد از هچ جمع‌آوری شدند. رشد وزنی بدن، خارج شدن اووسیت مدفوعی، درجه تخریب، نرخ مرگ و میر و pH سکوم بررسی شدند. تست ELISA برای تشخیص خوشه مولکول‌های سلول‌ T افتراقی (CD4+ و CD8+) استفاده شده و واکنش زنجیره‌ای پلیمراز رونوشت‌بردار معکوس (RT-PCR) برای تشخیص کموکاین‌های پیش التهابی (INFγ) و بیان سایتوکاین‌ها (IL-8، IL-15 و IL-17) بکار گرفته شدند. نتیجه نشان داد که مکمل‌سازی اسیدهای ارگانیک به ‌طور معنی‌داری رشد وزنی بدن را افزایش، ضریب تبدیل خوراک را بهبود، و درجات جراحات و شاخص اووسیت (0.05>P) در مقایسه با گروه کنترل P (آلوده شده غیر مکمل) را کاهش داد. نرخ مرگ و میر در گروه کنترل P بالاتر بود. pH روده کور معنی‌دار بود (0.05>P) و حداکثر در گروه کنترل P مشاهده شد. جوجه‌های تغذیه شده با 2 درصد DA و 1 درصد Lacplus درصد بالاتری از مولکول‌های سلول T CD4 داشتند، اما مولکول‌های سلول T CD8 کمتری داشتند، که ارائه کننده یک عملکرد محافظتی از این زیرمجموعه‌های سلول T در پاسخ ایمنی ذاتی در مقابل عفونت E. tenella است. کموکاین‌های روده کور و طحال و بیان mRNA سایتوکاین‌ها شامل INFγ، IL-8، IL-15 و IL-17 سطوح بالاتری از رونویسی در مقایسه با کنترل‌های N و P نشان دادند، که نشان می‌دهد محصولات اسید ارگانیک ممکن است با بهبود تولید آنها آثار محافظتی خود را اعمال کرده باشند. نتیجه گرفته شده که Activate® DA در سطوح 2 درصد و Laxplus در سطوح 1 درصد هم در روز 22 و هم در روز 35 بعد از هچ بهترین آثار ضدکوکسیدیال را نشان داده‌اند.

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