Effects of Different Antifreeze Protective Agents on Hu Sheep Semen Storage at 4 ˚C
محورهای موضوعی : CamelL. Zhang 1 , X. Sun 2 , Y. Kang 3 , T. Sohail 4 , X. Wang 5 , Y. Li 6
1 - College Animal Science and Technology, Yangzhou University, Yangzhou, China
2 - College Animal Science and Technology, Yangzhou University, Yangzhou, China
3 - College Animal Science and Technology, Yangzhou University, Yangzhou, China
4 - College Animal Science and Technology, Yangzhou University, Yangzhou, China
5 - College Animal Science and Technology, Yangzhou University, Yangzhou, China
6 - College Animal Science and Technology, Yangzhou University, Yangzhou, China
کلید واژه: glycerol, DMSO, computer‐assisted spermatozoa analysis, glycol, SMP, soy leci-thin,
چکیده مقاله :
The present study aimed to investigate the effects of different antifreeze protectants on the preservation of Hu sheep semen at 4 ˚C. The semen was diluted with Tris extender at room temperature, supplemented with dimethyl sulfoxide (DMSO) (0, 0.1%, 0.2%, 0.3%, 0.4% and 0.5%), glycerol (1.5%, 3.0%, 4.5% and 6.0%), glycol (2.50%, 5.00%, 7.50% and 10.00%), skimmed milk powder (SMP) (0.50%, 1.50%, 2.50% and 3.00%), soy lecithin (SL, 0.15%, 0.30%, 0.45% and 0.60%) and five optimal concentrations of antifreeze protectants (0.4% DMSO, 4.5% glycerol, 7.5% glycol, 1.5% SMP and 0.15% SL), and stored at 4 ˚C refrigerator. Spermatozoa motility parameters (spermatozoa viability, spermatozoa progressive motility, straight line velocity (VSL), curvilinear velocity (VCL) and average path velocity (VAP)) were evaluated during the preservation of semen. The addition of antifreeze protectants, especially the 0.4% DMSO, 4.5% glycerol, 7.50% glycol, 1.50% SMP and 0.15% SL exerted the best effects on spermatozoa viability and progressive motility compared to the spermatozoa without antifreeze protectants (control group). In the experiment of antifreeze protective agents with the optimal concentration, the spermatozoa viability and progressive motility of 1.5% SMP were significantly higher than those of other antifreeze groups during storage. In conclusion, the addition of 1.5% SMP to semen spermatozoa preserved at 4 ˚C refrigerator can most effectively enhance the semen preservation quality compared to the other optimal concentration of antifreeze protectant groups.
Alvarez R.A., Alvarez M., Anel-López A., Martínez-Rodríguez M., Martínez-Pastor M., Borragan S., Anel L. and de Paz P. (2013). The antioxidant effects of soybean lecithin- or low-density lipoprotein-based extenders for the cryopreservation of brown-bear (Ursus arctos) spermatozoa. Reprod. Fertil. Dev. 25(8), 1185-1193.
Blanco J.M., Long J.A., Gee G., Wildt D.E. and Donoghue A.M. (2011). Comparative cryopreservation of avian spermatozoa: benefits of non-permeating osmoprotectants and ATP on turkey and crane sperm cryosurvival. Anim. Reprod. Sci. 123(3), 242-248.
Chen H., Cheung M.P., Chow P.H., Cheung A.L., Liu W. and WS O. (2002). Protection of sperm DNA against oxidative stress in vivo by accessory sex gland secretions in male hamsters. Reproduction. 124(4), 491-499.
Dalmazzo A., Losano J.D.A., Rocha C.C., Tsunoda R.H., Angrimani D.S.R., Mendes C.M., Assumpção M.E.O.D.Á., Nichi M. and Barnabe V.H. (2018). Effects of soy lecithin extender on dog sperm cryopreservation. Anim Biotechnol. 29(3), 174-182.
Daly J., Smith H., McGrice H.A., Kind K.L. and van Wettere W.H.E.J. (2020). Towards improving the outcomes of assisted reproductive technologies of cattle and sheep, with particular focus on recipient management. Animals. 10(2), 293-308.
Del V., Gómez D., Holt W.V., Muiño B. and Cebrián P. (2012). Soy lecithin interferes with mitochondrial function in frozen-thawed ram spermatozoa. J. Androl. 33(4), 717-725.
Diaz J., Dorado J., Pereira B., Consuegra C., Ortiz I. and Hidalgo M. (2019). Is sperm cryopreservation in absence of permeable cryoprotectants suitable for subfertile donkeys? Reprod. Domest. Anim. 54(4), 102-105.
Esteves S.C., Humaidan P., Roque M. and Agarwal A. (2019). Female infertility and assisted reproductive technology. Panminerva Med. 61(1), 1-2.
Figueiredo C.C., Bisinotto D.Z., Brandão G.V.R., Umaña Sedó S. and Bisinotto R.S. (2020). Impact of assisted reproduction techniques on subsequent reproductive performance of dairy heifers and lactating cows. Theriogenology. 158, 97-104.
Forouzanfar M., Sharafi M., Hosseini S.M., Ostadhosseini S., Hajian M., Hosseini L., Abedi P., Nili N., Rahmani H.R. and Nasr-Esfahani M.H. (2010). In vitro comparison of egg yolk-based and soybean lecithin-based extenders for cryopreservation of ram semen. Theriogenology. 73(4), 480-487.
Gao D.Y., Lin S., Watson P.F. and Critser J.K. (1995). Fracture phenomena in an isotonic salt solution during freezing and their elimination using glycerol. Cryobiology. 32(3), 270-284.
Garde J.J., del Olmo A., Soler A.J., Espeso G., Gomendio M. and Roldan E.R. (2008). Effect of egg yolk, cryoprotectant, and various sugars on semen cryopreservation in endangered Cuvier's gazelle (Gazella cuvieri). Anim. Reprod. Sci. 108(3), 384-401.
Gilmore J.A., Liu J., Woods E.J., Peter A.T. and Critser J.K. (2000). Cryoprotective agent and temperature effects on human sperm membrane permeabilities: Convergence of theoretical and empirical approaches for optimal cryopreservation methods. Hum. Reprod. 15(2), 335-343.
Gloria A., Zambelli D., Carluccio A., Cunto M., Ponzio P. and Contri A. (2020). Is the protective effect of egg yolk against osmotic and cryogenic damage on dog spermatozoa dose-dependent? Anim. Reprod. Sci. 213, 1-11.
Gonzalez C., Trentin J.M., Carnevale E.M. and Graham J.K. (2019). Effects of extender, cryoprotectants and thawing protocol on motility of frozen-thawed stallion sperm that were refrozen for intracytoplasmic sperm injection doses. Theriogenology. 136, 36-42.
Guthrie H.D., Liu J. and Critser J.K. (2002). Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biol. Reprod. 67(6), 1811-1816.
Hassan M.M., Li X., Liu Y. and Qin J.G. (2017). Sperm cryopreservation in the spermcasting Australian flat oyster Ostrea angasi by a programmable freezing method. Cryobiology. 76, 119-124.
Hussain S.A., Lessard C. and Anzar M. (2013). A strategy for improvement of postthaw quality of bison sperm. Theriogenology. 79(1), 108-115.
Kmenta I., Strohmayer C., Müller-Schlösser F. and Schäfer-Somi S. (2011). Effects of a lecithin and catalase containing semen extender and a second dilution with different enhancing buffers on the quality of cold-stored canine spermatozoa. Theriogenology. 75(6), 1095-1103.
Küçük N., Aksoy M., Uçan U., Ahmad E., Naseer Z., Ceylan A. and Serin I. (2014). Comparison of two different cryopreservation protocols for freezing goat semen. Cryobiology. 68(3), 327-331.
Kundu C.N., Chakraborty J., Dutta P., Bhattacharyya D., Ghosh A. and Majumder G.C. (2000). Development of a simple sperm cryopreservation model using a chemically defined medium and goat cauda epididymal spermatozoa. Cryobiology. 40(2), 117-125.
Lohmann W., Fowler C.F., Moss A.J. and Perkins W.H. (1964). Some remarks about the effect of glycerol on cells during freezing and thawing: electron-spin resonance investigations concerning this effect. Experientia. 20(5), 290-301.
Lovelock J.E. (1953). The mechanism of the protective action of glycerol against haemolysis by freezing and thawing. Biochim. Biophys. Acta. 11(1), 28-36.
Maxwell W.M. and Salamon S. (1979). Fertility of frozen-thawed boar semen. Australian J. Biol. Sci. 32(2), 243-249.
Mehdipour M., Daghigh Kia H., Najafi A., Vaseghi Dodaran H. and García-Álvarez O. (2016). Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender. Cryobiology. 73(3), 297-303.
Moore A.I., Squires E.L., Bruemmer J.E. and Graham J.K. (2006). Effect of cooling rate and cryoprotectant on the cryosurvival of equine spermatozoa. J. Equine Vet. Sci. 26, 215-218.
Najafi A., Daghigh-Kia H., Dodaran H.V., Mehdipour M. and Alvarez-Rodriguez M. (2017). Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation. Anim. Reprod. Sci. 177, 35-41.
Polge C. (1953). The storage of bull semen at low temperatures. Vet. Rec. 65, 557-569.
Pursel V.G. and Johnson LA. (1975). Freezing of boar spermatozoa: fertilizing capacity with concentrated semen and a new thawing procedure. J. Anim. Sci. 40(1), 99-102.
Reed M.L., Ezeh P.C., Hamic A., Thompson D.J. and Caperton C.L. (2009). Soy lecithin replaces egg yolk for cryopreservation of human sperm without adversely affecting postthaw motility, morphology, sperm DNA integrity, or sperm binding to hyaluronate. Fertil. Steril. 92(5), 1787-1790.
Rezaie F.S., Hezavehei M., Sharafi M. and Shahverdi A. (2021). Improving the post-thaw quality of rooster semen using the extender supplemented with resveratrol. Poult. Sci. 100(9), 1-21.
Rodriguez M. (2012). Assisted reproductive techniques for cattle breeding in developing countries: A critical appraisal of their value and limitations. Reprod. Domest. Anim. 47(1), 21-26.
Rosato M.P. and Iaffaldano N. (2013). Cryopreservation of rabbit semen: comparing the effects of different cryoprotectants, cryoprotectant-free vitrification, and the use of albumin plus osmoprotectants on sperm survival and fertility after standard vapor freezing and vitrification. Theriogenology. 79(3), 508-516.
Rota A., Milani C., Cabianca G. and Martini M. (2006). Comparison between glycerol and ethylene glycol for dog semen cryopreservation. Theriogenology. 65(9), 1848-1858.
Shin J.M., Gwak J.W., Kamarajan P., Fenno J.C., Rickard A.H. and Kapila Y.L. (2016). Biomedical applications of nisin. J. Appl. Microbiol. 120(6), 1449-1465.
Silva E.C., Cajueiro J.F., Silva S.V., Vidal A.H., Soares P.C. and Guerra M.M. (2012). In vitro evaluation of ram sperm frozen with glycerol, ethylene glycol or acetamide. Anim. Reprod. Sci. 132(3), 155-158.
SPSS Inc. (2011). Statistical Package for Social Sciences Study. SPSS for Windows, Version 25. Chicago SPSS Inc., USA.
Swelum A.A., Mansour H.A., Elsayed A.A. and Amer H.A. (2011). Comparing ethylene glycol with glycerol for cryopreservation of buffalo bull semen in egg-yolk containing extenders. Theriogenology. 76(5), 833-842.
Tvrdá E., Bučko O., Rojková K., Ďuračka M., Kunová S., Kováč J., Benko F. and Kačániová M. (2021). The efficiency of selected extenders against bacterial contamination of boar semen in a swine breeding facility in western slovakia. Animals. 11(11), 3320-3331.
Vernet P., Fulton N., Wallace C. and Aitken R.J. (2001). Analysis of reactive oxygen species generating systems in rat epididymal spermatozoa. Biol. Reprod. 65(4), 1102-1113.
Vicente J.S. and Viudes C. (1996). A sucrose-DMSO extender for freezing rabbit semen. Reprod. Nutr Dev. 36(5), 485-492.
Wu Z., Zheng X., Luo Y., Huo F., Dong H., Zhang G., Yu W., Tian F., He L. and Chen J. (2015). Cryopreservation of stallion spermatozoa using different cryoprotectants and combina soybean lecithin as an alternative to avian egg yolk in the cryopreservation of fish sperm. Cryobiology. 67(1), 91-94.
Zhang L., Wang Y., Sohail T., Kang Y., Niu H., Sun X., Ji D. and Li Y. (2021). Effects of taurine on sperm quality during room temperature storage in Hu sheep. Animals. 11(9), 2725-2732.
Zhao J.Q., tions of cryoprotectants. Anim. Reprod. Sci. 163, 75-81.
Wündrich K., Paasch U., Leicht M. and Glander H.J. (2006). Activation of caspases in human spermatozoa during cryopreservation-an immunoblot study. Cell Tissue Bank. 7(2), 81-90.
Yildiz C., Bozkurt Y. and Yavas I. (2013). An evaluation of Xiao G.L., Zhu W.L., Fang D., Li N., Han C.M. and Gao Q.H. (2021). Ram semen preserved at 0 °C with soybean lecithin Tris-based extender substituted for egg yolk. Anim. Biosci. 34(2), 192-197.
