Serum Protein Profile of Lori-Bakhtiari Ewes in Relation to Age, Body Weight, Birth Type and Birth Season

محورهای موضوعی : Camel

H. Mohammadi ¹ (Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran)
P. Zamani ² (Department of Animal Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran)
S.Z. Mirhoseini ³ (Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran)

تاریخ دریافت : 1399/10/16 تاریخ پذیرش : 1400/04/10 تاریخ انتشار : 1400/12/10

کلید واژه: Health, Sheep, body weight, breeding value, Immunology,

چکیده مقاله :

Serum protein profile could be considered as a useful indicator of physiological and pathological conditions, but several factors may interfere with the interpretation. This study was conducted to evaluate serum protein fractions in association with age, body weight, birth type and birth season in a meat-type sheep. Blood samples were randomly taken from 96 healthy Lori-Bakhtiari ewes in the Shooli breeding station in Iran. The sampled animals randomly belonged to different age, birth type, birth season and body weight groups. Total protein and other fractions were determined by Biuret and electrophoresis methods, respectively. IgG levels were measured by ELISA method. Total protein, albumin, total globulins, albumin/globulins ratio, α1, α2, β and γ globulins and IgG levels averages were 5.82, 2.35, 3.47, 0.69, 0.06, 0.42, 0.15, 2.82 and 1.56 g/dL, respectively. Total protein had high correlations with total and γ globulins, while albumin and IgG were almost independent from other fractions. Levels of serum protein fractions in adults were also independent from body weights at different ages. Serum protein profile was significantly affected by age and birth season (P<0.05), where by older ewes had lower levels of α2, γ and total globulins and higher albumin/globulin ratios. The winter-born ewes significantly had higher γ globulins levels and probably more immune system activities, compared to the spring-born ewes. Body weight and birth type did not have any significant effect on the studied serum protein fractions. This study suggested that the age and birth season should be considered for interpretation of the serum protein profiles.

منابع و مأخذ:

Alberghina D., Casella S., Vazzana I., Ferrantelli V., Giannetto C. and Piccione G. (2010). Analysis of serum proteins in clinically healthy goats (Capra hircus) using agarose gel electrophoresis. Vet. Clin. Pathol. 39, 317-321.

Al-Hadithy H.A. and Badawi N.M. (2015). Determination of serum proteins and glucose concentrations in clinically normal and anemic Awassi sheep. World’s Vet. J. 5, 1-6.

Almasi M., Zamani P., Mirhoseini S.Z. and Moradi M.H. (2020). Genome-wide association study for weaning traits in a meat type sheep. Ann. Anim. Sci. 20, 811-824.

Alves A.C., Alves N.G., Ascari I.J., Junqueira F.B., Coutinho A.S., Lima R.R., Pérez J.R., De Paula S.O., Furusho-Garcia I.F. and Abreu L.R. (2015). Colostrum composition of Santa Inês sheep and passive transfer of immunity to lambs. J. Dairy Sci. 98, 3706-3716.

Aynalem Gemechu G. and Kibeb L. (2017). Effect of age, sex and altitude on the normal physiological and biochemical parameters in apparently healthy local breed sheep in Shebedinodistrict in Sidam zone, Ethiopia. BioChem. Indian J. 11, 122-128.

Borjesson D.L., Christopher M.M. and Boyce W.M. (2000). Biochemical and hematologic reference intervals for free-ranging desert bighorn sheep. J. Wildlife Diseas. 36, 294-300.

Burtis C.A. and Bruns D.E. (2014). Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. Elsevier, Amsterdam, The Netherlands.

Carlos M.M.L., Leite J.H.G.M., Chaves D.F., Vale A.M., Façanha D.A.E., Melo M.M. and Soto-Blanco B. (2015). Blood parameters in the Morada Nova sheep: Influence of age, sex and body condition score. J. Anim. Plant Sci. 25, 950-955.

Da Cruz R.E.S., Rocha F.M., Sena C.V.B., Noleto P.G., Guimarães E.C., Galo J.A. and Mundim A.V. (2017). Effects of age and sex on blood biochemistry of Dorper lambs, Semin. Cie. Agr. 38, 3085-3093.

Durak M.H., Erkan R.E.C., Çelik R., Yokuş B., Kurt D. and Gürgöze S. (2015). The effects of age and gender on some biochemical serum parameters in Zom sheep raised in the vicinity of Karacadağ. Israel J. Vet. Med. 70(2), 33-39.

Eckersall P.D. (2008). Proteins, proteomics, and the dysproteinemias. Pp. 117-155 in Clinical Biochemistry of Domestic Animals. J.J. Kaneko, J.W. Harvey and M.L. Bruss, Eds. Academic Press, San Diego, USA.

Esmaeilnejad B., Tavassoli M., Asri-Rezaei S., Dalir-Naghadeh B. and Pourseyed S.H. (2014). Evaluation of serum total protein concentration and protein fractions in sheep naturally infected with Babesia ovis. Comp. Clin. Pathol. 23, 151-155.

Geffré A., Friedrichs K., Harr K., Concordet D., Trumel C. and Braun J.P. (2009). Reference values: A review. Vet. Clin. Pathol. 38, 288-298.

Ghasemi M., Zamani P., Vatankhah M. and Abdoli R. (2019). Genome-wide association study of birth weight in sheep. Animal. 13, 1797-1803.

Ignjatovic V., Lai C., Summerhayes R., Mathesius U., Tawfilis S., Perugini M.A. and Monagle P. (2011). Age-related differences in plasma proteins: How plasma proteins change from neonates to adults. PLoS One. 6(2), e17213.

Jin Y.M., Zhang X.Q., Badgery W.B., Li P. and Wu J.X. (2019). Effects of winter and spring housing on growth performance and blood metabolites of Pengbo semi-wool sheep in Tibet, Asian-Australasian J. Anim. Sci. 32, 1630-1639.

Khazraei Nia P., Mojabi A., Abasalipour Kabireh M. and Nowrouzian I. (1998). Determination of serum total protein and different blood serum protein fractionslevels using cellulose acetate electrophoresis method in Iranian sheep. Anim. Sci. J. (Pajouhesh and Sazandegi). 38, 118-121.

Koushki R., Mansoori Yarahmadi A., Khaldari M., Fakhraei L. and Karkoodi K. (2019). Milk yield and blood metabolite profile in late pregnancy in Lori ewes receiving diets containing undegradable protein sources. Iranian J. Appl. Anim. Sci. 9, 643-650.

Lepherd M.L., Canfield P.J., Hunt G.B. and Bosward K.L. (2009). Haematological, biochemical and selected acute phase protein reference intervals for weaned female Merino lambs. Australian Vet. J. 87, 5-11.

Merlot A.M., Kalinowski D.S. and Richardson D.R. (2014). Unraveling the mysteries of serum albumin - more than just a serum protein. Front. Physiol. 5, 299-305.

Meyer K. (2007). WOMBAT: A tool for mixed model analyses in quantitative genetics by restricted maximum likelihood (REML). J. Zhejiang Univ. Sci. B. 8, 815-821.

Miglio A., Antognoni M.T., Maresca C., Moncada C., Riondato F., Scoccia E. and Mangili V. (2015). Serum protein concentration and protein fractions in clinically healthy Lacaune and Sarda sheep using agarose gel electrophoresis. Vet. Clin. Pathol. 44, 564-569.

Murariu O.C., Lazǎr R., Murariu F., Paul C. and Boişteanu P.C. (2014). Variation of biochemical parameters of ovine blood according to genotype and sex after the stalling period, Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Anim. Sci. Biotechnol. 71, 73-75.

Nagy O., Tóthová C., Nagyová V. and Kováč G. (2015). Comparison of serum protein electrophoretic pattern in cows and small ruminants. Acta Vet. Brno. 84, 187-195.

Nagy O., Tothova C., Nagyova V., Kovac G. and Posivak J. (2014). Changes in the serum protein electrophoretic pattern in lambs during the first month of life. Acta Vet. Brno. 83, 187-193.

Nagyová V., Arfuso F., Rizzo M., Nagy O. and Piccione G. (2017). Stability of ovine serum protein fractions under different storage conditions. J. Vet. Diagn. Invest. 29, 312-315.

Nagyová V., Arfuso F., Rizzo M., Zanghì E., Nagy O. and Piccione G. (2016). Stability of total proteins and their electrophoretic fractions in goat serum (Capra hircus), maintained under different condition. Small Rumin. Res. 144, 145-148.

Nazifi S. and Mostaghni K. (1995). Electrophoresis of serum proteins in Ghezel and Mehraban sheep. (Pajouhesh and Sazandegi). 29, 118-125.

Njidda A.A., Shuai’bu A.A. and Isidahomen C.E. (2014). Haematological and serum biochemical indices of sheep in semi-arid environment of northern Nigeria. Global J. Sci. Front. Res. D Agric. Vet. 14(2), 49-56.

NRC. (2007). Nutrient Requirements of Small Ruminants, Sheep, Goats, Cervids, and New World Camelids. National Academy Press, Washington, D.C., USA.

O'Connell T.X., Horita T.J. and Kasravi B. (2005). Understanding and interpreting serum protein electrophoresis. American Family Phys. 71, 105-112.

Piccione G., Arfuso F., Faggio C., Casella S., Zumbo A. and Panzera M. (2013). Serum proteins profile in Comisana lambs during the first month of life. Arch. Anim. Breed. 56, 742-750.

Piccione G., Casella S., Giannetto C., Panzera M., Pennisi P. and Alberghina D. (2014). Influence of short-term storage on electrophoretic profile of bovine serum proteins. J. Appl. Anim. Res. 42, 123-125.

Rahman M.K., Islam S., Ferdous J., Uddin M.H., Hossain M.B., Hassan M.M. and Islam A. (2018). Determination of hematological and serum biochemical reference values for indigenous sheep (Ovies aries) in Dhaka and Chittagong districts of Bangladesh. Vet. World. 11, 1089-1093.

Sabes A.F., Girardi A.M., Fagliari J.J., de Oliveira J.A. and Marques L.C. (2017). Serum proteinogram in sheep with acute ruminal lactic acidosis. Int. J. Vet. Sci. Med. 5, 35-40.

SAS Institute (2013). Users Guide, Version 9.4: Statistics. SAS Institute, Cary, NC, USA.

Tothova C., Mihajlovičová X. and Nagy O. (2017). The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases. Acta Vet. 67, 178-190.

Tothova C., Nagy O. and Kovac G. (2016a). Serum proteins and their diagnostic utility in veterinary medicine: A review. Vet. Med. 61, 475-496.

Tothova C., Nagy O., Nagyova V. and Kovac G. (2016b). The concentrations of selected blood serum proteins in calves during the first three months of life. Acta Vet. Brno. 85, 33-40.

Tothova C., Nagy O., Seidel H. and Kovac G. (2010). The effect of storage on the protein electrophoretic pattern in bovine serum. Iranian J. Vet. Sci. Technol. 2, 77-84.

Tothova C., Nagy O., Seidel H. and Kovac G. (2013). Serum protein electrophoretic pattern in clinically healthy calves and cows determined by agarose gel electrophoresis. Comp. Clin. Pathol. 22, 15-20.

Tschuor A.C., Riond B., Braun U. and Lutz H. (2008). Hematological and clinical biochemical reference values for adult goats and sheep. Schweiz. Archiv fur Tierheilkd. 150, 287-295.

Zamani P., Salehian Dehkordi H., Doosti A. and Abdolmohammadi A. (2016). Ovine DRB1 polymorphism and its associations with body weight, milk contents and immunological parameters. Ann. Anim. Sci. 16, 425-438.

Zeder M.A. (1999). Animal domestication in the Zagros: A review of past and current research. Paléorient. 25(2), 11-26.