Effect of Essential Oils of Zataria multiflora, Eucalyptus globolus and Their Combination on Fermentation Parameters Using Merghoz Goat Rumen Liquor
محورهای موضوعی : Camelس. میرزایی چشمه‌گچی 1 , م.م. معینی 2 , ف. هژبری 3 , م.ا. نوریان سرور 4
1 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
2 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
3 - عضو هیات علمی د انشگاه رازی
4 - Department of Animal Science, Faculty of Agriculture, Razi University, Kermanshah, Iran
کلید واژه: partitioning factor, methane production, organic matter digestibility, protozoa popu-lation,
چکیده مقاله :
In this experiment, different doses of Zataria multiflora (ZM) and Eucalyptus globolus (EG) essential oils (EOs) (0, 100, 250, 500, 750 and 1000 µg/mL) and a mixture of them were added to alfalfa hay incubated with buffered rumen liquor of Merghoz goat to assess in vitro gas production, rumen fermentation and protozoa population. In all treatments the asymptotic gas volume (B) and the rate constant (c) were decreased while the lag phase was increased. There were no effects of ZM and EG on pH, but in high doses of ZE pH was higher than that of control (P<0.001). The ammonia-N concentration was decreased due to addition of combination of EOs (P<0.01) and volatile fatty acid concentration was reduced (P<0.01) following incorporation of EOs. Gas production and organic matter digestibility were decreased (P<0.01) 24 h after incubation, whereas the partitioning factor was increased. Metabolizable energy was decreased, (P<0.05). By inclusion of EOs, total protozoa population and individual genera reduced (P<0.001). The results revealed that EOs of ZM and EG could be potentially used to modulate rumen fermentation, but using them at high level doses have anti-protozoal effects.
در این مطالعه، سطوح مختلف روغنهای اسانسی آویشن شیرازی، اکالیپتوس (0، 100، 250، 500، 750 و 1000 میکروگرم در میلیلیتر) و مخلوط یکسان آنها به علوفه یونجه همراه با مایع شکمبه بافری شده بز مرخز اضافه وگرمخانه گذاری شد تا تولید گاز، تخمیر شکمبه و جمعیت پروتوزوآ در شرایط آزمایشگاهی مورد مطالعه قرار گیرد. پتانسیل تولید گاز (B) و سرعت تخمیر (C) در تمام تیمارهای آزمایشی کاهش یافت درحالیکه فاز تأخیر (L) افزایش نشان داد. آویشن شیرازی و اکالیپتوس تأثیری بر pH نداشتند ولی در سطوح بالای مخلوط روغنهای اسانسی نسبت به شاهد، pH بالاتر بود (001/0>P). تولید نیتروژن آمونیاکی به سبب افزودن مخلوط روغنهای اسانسی کاهش یافت (01/0>P) و غلظت کل اسیدهای چرب فرار با اضافه نمودن روغنهای اسانسی آویشن شیرازی و اکالیپتوس کاهش نشان داد (01/0>P). گاز تولیدی 24 ساعته، تولید متان و میزان ماده آلی تجزیه شده کاهش یافت (01/0>P)، درحالیکه ضریب تفکیکپذیری در همه تیمارها افزایش نشان داد. انرژی متابولیسمی کاهش یافت (05/0>P). افزودن روغن اسانسی سبب کاهش تعداد پروتوزوآی کل و جنسهای مختلف پروتوزوآ شد (001/0>P). نتایج نشان داد که روغنهای اسانسی آویشن شیرازی و اکالیپتوس میتوانند در تعدیل تخمیر شکمبه مؤثر باشند ولی در سطوح بالا، دارای اثر ضد پروتوزوآیی می‌باشند.
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