Environmental and Genetic Factors Affecting on Semen Quality in Iranian Holstein Bulls

محورهای موضوعی : Camel

م.و. قاسمی ¹ ( Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran )
ا. قربانی ² ( Department of Animal Science, Shabestar Branch, Islamic Azad University, Shabestar, Iran )

تاریخ دریافت : 1391/11/08 تاریخ پذیرش : 1392/02/11 تاریخ انتشار : 1392/12/10

کلید واژه: genetic, semen quality, Iranian Holstein bulls, Environmental factor,

چکیده مقاله :

The objective of this study was to evaluate the importance of environmental and genetic factors affecting semen quality; sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh motile sperm in each milt ejaculation (NFMSE), motility before and after the freezing (MBATF) and number of post thaw motile sperm in each milt ejaculation (NPTMSE). We used polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in 83 bulls belonging to two progeny test centers.The BB genotype was omitted from analysis.Season of collection had a significant effect on sperm volume (SV)(P<0.006), sperm concentration (SPCO)(P<0.0038), fresh sperm motility (FSM)(P<0.0001), post thaw sperm motility (PTSM)(P<0.001), total post thaw motile sperm (TPTMS)(P<0.0075), number of produced payout (NPP)(P<0.0247), number of fresh motile sperm in each milt ejaculation (NFMSE)(P<0.0012) and motility before and after the freezing (MBATF)(P<0.0001) but did not significantly affect TS (P<0.438), total post thaw motile sperm (TPTMS)(P<0.3606) and number of post thaw motile sperm in each milt ejaculation (NPTMSE)(P<0.1106). The interaction of seasons with follicle-stimulating hormone and semen quality traits only significantly affect sperm volume (SV)(P<0.0435), sperm concentration (SPCO)(P<0.008) and number of produced payout (NPP)(P<0.0119). Genetics and environmental factors clearly contribute to semen production in Holstein bulls.

چکیده انگلیسی:

هدف از این مطالعه، ارزیابی تأثیرعوامل محیطی و ژنتیکی مؤثر بر صفات کیفیت اسپرم، حجم اسپرم، جمعیت اسپرم در هر میلی‌لیتر انزال، کل جمعیت اسپرم در هر انزال، جنبندگی اسپرم تازه، تعداد اسپرم تازه جنبا در هر میلی‌لیتر انزال، کل جمعیت اسپرم تازه متحرک در هر انزال، جنبندگی اسپرم بعد از یخ‌گشایی، تعداد اسپرم تازه جنبا در هر میلی‌لیتر انزال بعد از یخ‌گشایی، کل جمعیت اسپرم زنده بعد از یخ‌گشایی، تعداد پایت تولیدی در هر انزال و نسبت جنبندگی قبل و بعد از انجماد بود. از 83 گاو نر متعلق به دو مرکز آزمون نتاج نمونه خون و اسپرم تهیه و با استفاده از روش PCR_RFLP تعیین ژنوتیپ شدند. اثر فصل بر تعداد کل اسپرم متحرک بعد از یخ‌گشایی (0075/0>P)، نسبت تحرک قبل و بعد از انجماد (0001/0>P)، تعداد دز اسپرم تولیدی ‌(0247/0>P)، تحرک بعد از یخ‌گشایی (0001/0>P)، تعداد اسپرم متحرک به ازای هر میلی‌لیتر انزال بعد از انزال (0012/0>P)، تحرک بعد از انزال (0001/0>P)، جمعیت اسپرم (0038/0>P) و حجم انزال (006/0>P) معنی‌دار بود اما، با صفات تعداد کل اسپرم (438/0>P)، تعداد کل اسپرم متحرک بعد از انزال (3606/0>P) و تعداد اسپرم متحرک بعد از یخ‌گشایی در هر میلی‌لیتر انزال (1106/0>P) معنی‌دار نبود و اثر متقابل فصل اسپرم‌گیری با ژن هورمون محرک فولیکول تنها با حجم انزال (0435/0>P)، جمعیت اسپرم (0080/0>P)، تعداد اسپرم متحرک به ازای هر میلی‌لیتر انزال بعد از انزال (0117/0>P) و تعداد دز اسپرم تولیدی (0119/0>P) معنی‌دار بود. نتایج این تحقیق نشان داد که تقابل عوامل ژنتیکی و محیطی به ‌طور قابل توجهی بر روی تولید اسپرم در گاوهای نر هلشتاین تأثیر می‌گذارند.

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