A Review of Microsatellite Marker Usage in the Assessment of Genetic Diversity of Camelus

محورهای موضوعی : Camel

M.M. Musthafa ¹ ( Institute of Biological Science, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia )

تاریخ دریافت : 1392/10/17 تاریخ پذیرش : 1393/02/10 تاریخ انتشار : 1393/12/10

کلید واژه: Biodiversity, genetic diversity, Conservation, camel, Microsatellite,

چکیده مقاله :

Camels have been regarded as the desert ship and they play multi-utility role in the world. Estimation of genetic parameters is foremost step towards managing the genetic resources for their conservation and sustainable utilization. Microsatellite markers have been extensively used in cattle, sheep, goat and camels. However, genetic characterization studies on camels has been poorly recorded. There has been a rapid increase in amount of molecular data produced from indigenous camel populations, which clearly shows awareness among the scientific community. Based on the studies carried out in Australia, Kenya, Saudi Arabia, Canary Islands, India, Egypt and Tunisia the camels have shown very wide genetic diversity via the predefined microsatellite markers. It is highly recommended that to use following microsatellite markers to find the highly informative heterozygosity data: YWLL08, YWLL09, YWLL38, YWLL44, YWLL59, VOLP03, VOLP08, VOLP10, VOLP32, VOLP67, LCA66, CVRL01, CVRL05, CVRL06, CVRL07 and CMS50. These markers have shown a high level of allelic richness and polymorphic information content. Therefore, future genetic diversity analysis on camel can be based on these highly useful markers.

چکیده انگلیسی:

شترها به عنوان کشتی­های صحرا شناخته شده و کاربردهای مختلفی در جهان دارند. برآورد پارامترهای ژنتیکی نخستین گام در مدیریت منابع ژنتیکی برای حفاظت و استفاده مناسب از آنها است. نشانگرهای ریزماهواره به­ طور وسیعی در گاو، گوسفند، بُز و شتر به کار گرفته شده­اند. ولی مطالعات صورت گرفته در زمینه تعیین خصوصیات ژنتیکی بر روی شترها اندک است. افزایش سریعی در حجم داده­های مولکولی حاصل از جمعیت­های شتر بومی مشاهده می­شود. این موضوع حاکی از آن است که سطح آگاهی از این موضوع در جوامع علمی مناسب است. بر مبنای مطالعات صورت گرفته در استرالیا، کنیا، عربستان سعودی، جزایر قناری، هندوستان، مصر و تونس، تنوع ژنتیکی نشانگرهای ریزماهواره در شترها از سطح بالایی برخوردار است. برای یافتن داده­های هتروزیگوسیتی مفید توصیه می­شود که نشانگرهای ریزماهواره YWLL08، YWLL09، YWLL38، YWLL44، YWLL59، VOLP30، VOLP08، VOLP10، VOLP32، VOLP67، LCA66، CVRL01، CVRL05، CVRL06، CVRL07 و CMS50 استفاده گردد. این نشانگرها سطح بالایی از غنای آللی و محتوای اطلاعات چند‌شکلی را نشان می­دهند. بنابراین آنالیزهای تنوع ژنتیکی بر روی شترها در آینده می­تواند بر مبنای این نشانگرهای بسیار مفید صورت گیرد.

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