Chemical composition and antibacterial activity of essential oil from two Thymus species
محورهای موضوعی : مجله گیاهان دارویی
امین هادی پناه
1
(گروه باغبانی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران)
مجتبی خرمی
2
(گروه باغبانی، دانشگاه آزاد اسلامی واحد علوم و تحقیقات تهران، تهران، ایران)
کلید واژه: Staphylococcus aureus, Antibacterial activity, Thymus daenensis Celak, Chemical constitutes, hymus vulgaris L,
چکیده مقاله :
Background & Aim: Staphylococcus aureus is one of the bacterial pathogens and the most important factor in hospitalized infections in human community. This bacterium is becoming resistance to chemical drugs quickly. Lots of countries are trying to find new compositions as a suitable replacement for these antibiotics. The aim of this study was antibacterial activity and chemical composition of (Thymus vulgaris L.) and (Thymus daenensis Celak) cultivated in Iran. Experimental: The aerial parts of T. vulgaris and T. daenensis were collected from stages beginning of blooming set at Estahban (Fars province) in South Iran, during 2014.The essential oil was extracted by a Clevenger approach and analyzed using GC/MS. This essential oil was examined on S. aureus with different sensitivity levels to vancomycin, methicilin and oxacillin antibiotics. Results & Discussion: In T. vulgaris and T. daenensisoil, 28, 26 compounds were identified, respectively. The major constituents of the T. vulgaris oil were thymol (63.14%), p-cymene (9.45%), g-terpinene (8.67%) and carvacrol (3.14%).The major constituents of the T. daenensis oil were; thymol (71.2%), g-terpinene (8.81%), p-cymene (3.8%) and carvacrol (2.4%). According to these results, MIC and MBC of T. vulgaris and T. Daenensis oil on S. aureus were estimated 0.1 to 0.5 micro l/ml respectively. Industrial and practical recommendations: In conclusion, the results obtained in our study indicated that Thymus daenensis oil had a high suppressing and fatal effects.
مقدمه و هدف :استافیلوکوکوس اورئوس (Staphylococcus aureus) ازجمله باکتریهای بیماریزا و از عوامل مهم عفونتهای بیمارستانی درجوامع انسانی می باشد. این باکتری نسبت به داروهای شیمیایی به سرعت درحال مقاوم شدن می باشد و در بسیاری از کشورها تلاش زیادی جهت یافتن ترکیبهای جدید به عنوان جایگزین مناسب این آنتی بیوتیکها انجام می شود. هدف از این تحقیق شناسایی ترکیبات شیمیایی و فعالیت ضد باکتری اسانس 2 گونه آویشن (Thymus vulgaris L.) و (Thymus daenensis Celak)کشت شده در شرایط آب و هوایی استان فارس بود. روش تحقیق: قسمتهای هوایی آویشن باغی و آویشن دنایی در مرحله آغاز گلدهی در منطقه استهبان (استان فارس) در جنوب ایران، در سال 1393 جمع آوری شد. استخراج اسانس به روش تقطیر با آب و به کمک دستگاه اسانس گیری طرح کلونجر صورت گرفت و میزان اسانس به وسیله دستگاه کروماتوگرافی گازی متصل به طیف سنج جرمی (GC/MS) مورد تجزیه و شناسایی قرار گرفت. اسانس استافیلوکوکوساورئوس با سطوح مختلف آنتی بیوتیک وانکومایسین، متیسیلین و اکساسیلین مورد بررسی قرار گرفت. نتایج و بحث:نتایج بدست آمده از این تحقیق نشان داد که به ترتیب 28 و26 ترکیب در اسانس آویشن باغی و آویشن دنایی شناسایی شدند. ترکیبات اصلی اسانس آویشن باغی شامل تیمول (14/63%)، پارا-سیمین (45/9%)، گاما-ترپینن (67/8%) و کارواکرول (14/3%) حاصل شد. ترکیبات اصلی اسانس آویشن دنایی شامل تیمول (2/71%)، گاما-ترپینن (81/8%)، پارا-سیمین (8/3%) و کارواکرول (4/2%) بدست آمد. نتایج حاکی ازآن بود که MIC و MBC اسانس آویشن باغی و آویشن دنایی به ترتیب تاثیر بازدارندگی رشد 1/0 تا 5/0 میکرولیتردر یک میلی لیتر بر باکتری استافیلوکوکوس اورئوس دارند. توصیه کاربردی/صنعتی: اسانس آویشن دنایی به طور محسوسی ویژگی ضدباکتری بارزتری نسبت به اسانس آویشن باغی از خود نشان داد.
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