Comparative study of the effect of Eucalyptus extract on Candida albicans and human pathogenic bacteria
محورهای موضوعی : مجله گیاهان داروییزهرا دهباشی 1 , فروغ فرقانی 2 , سید کاظم صباغ 3 , سعیده سعیدی 4
1 - گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران
2 - گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی زابل، زابل، ایران
3 - گروه زیست شناسی، دانشکده علوم، دانشگاه یزد، یزد، ایران
4 - کارشناس میکروبیولوژی، پژوهشکده زیست فناوری کشاورزی، دانشگاه زابل، زابل، ایران
کلید واژه: Minimum inhibitory concentration, Candida albicans, Anti-fungal activity, Anti-microbial activity, E. globules,
چکیده مقاله :
Background & Aim:During recent decades, infections disease resulting from opportunistic fungi such as Candida albicans and resistance increasing of human pathogenic bacteria to current antibiotics has led to many problems for treating of these diseases. The aims of the present study was to investigate anti-fungi and anti-bacterial effects of Eucalyptus extract on Candida albicans species isolated from clinical samples and some standard human pathogenic bacteria. Experimental: Eucalyptus extract was provided using rotary apparatus and maceration method. Thirty isolates of Candida albicans were isolated from referred patients to gynecologist and then were purified and identified by valid keys. Finally, 30 isolated were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of eucalyptus extract against 5 standard bacteria inculding: Staphylococcus aureu, Shigella dysenteriae, Listeria monocytogenes, Vibrio cholera and Bacillus cereus were evaluated using micro broth dilution method. Results: Our results indicated that in compare to bacterial strains, fungus isolates showed more sensitivity to eucalyptus extract. The highest and lowest MIC of extract was recorded at 12.5 and 3.1 ppm for S. aureus and B. cereus, respectively. As MIC, the maximum MBC (20ppm) and minimum MBC (5ppm) of extract was recorded fro S. aureus and B. cereus respectively. Minimum inhibitory concentration of extract to fungal growth inhibitory was 50 ppm, whereas the maximum inhibiting concentration was 150 ppm. Recommended applications/industries: The positive and interesting results suggest the essential oil of E. globulus could be exploited antibiotic for the treatment of candidiasous disease caused by Candida albicans fungi, and some human pathogenic bacteria studied in this work.
مقدمه و هدف: در طی سالیان اخیر، بیماریهای عفونی ناشی از قارچهای فرصت طلب مانند Candida albicans و افزایش مقاومت باکتریهای بیماریای انسانی به آنتی بیوتیک های رایج منجر به بروز مشکلات زیادی در ارتباط با درمان این بیماری ها شده است. هدف از مطالعه حاضر بررسی اثرات ضد قارچی و باکتریایی عصاره گیاه اکالیپتوس بر علیه جدایه های Candida albicans جدا شده از نمونه های کلینیکی و تعدادی از جدایه های استاندارد از باکتری های بیماری زای انسانی بود. روش تحقیق: عصاره اکایپتوس با استفاده از از دستگاه روتاری و روش خیساندن تهیه گردید. تعداد 30 جدایه از Candida albicans از بیماران مراجعه کننده به متخصص زنان و زایمان جدا شده و پس از خالص سازی به وسیله کلید های معتبر شناسایی شدند. در نهایت تعداد 30 جدایه برای بررسی اثر بازدارندگی رشد عصاره مورد استفاده قرار گرفتند. حداقل غلظت کشندگی و حداقل غلظت بازدارندگی عصاره اکالیپتوس علیه 5 سویه استاندارد باکتریایی شامل Staphylococcus aureu, Shigella dysenteriae, Listeria monocytogenes, Vibrio cholera and Bacillus cereusبا روش موردارزیابی قرار گرفت. نتایج و بحث: نتایج ما نشان داد که در مقایسه با سویه های باکتریایی، جدایه های قرچی حساسیت بیشتری را نسبت به عصاره اکالیپتوس دارا می باشد. بیشترین و کمترین اثر کشندگی عصاره به ترتیب برای غلظت های 12.5 و 3.1 پی پی ام و علیه S. aureus و B. cereusثبت گردید. ماندد اثر کشندگی، بیشترین اثر بازدارندگی (20 پی پی ام) و کمترین اثر بازدارندگی (5پی پی ام) علیه S. aureus و B. cereusثبت گردید. حداقل غلظت کشندگی عصاره برای جلوگیری از رشد قارچی در غلظت 50 پی پی ام ثبت در حالی که بیشترین غلظت برای اثر بازدارندگی 150 پی پی ام ثبت گردید. توصیه های کاربردی/ صنعتی: نتایج این مطالعه نشان داد که غلظت بالای اکالیپتوس قادر به بازدارندگی رشد قارچ Candida albicansمی باشد و در نتیجه مطالعات بیشتری در جهت آنالیز ترکیبات موثر در عصاره این گیاه می تواند انجام شود.
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