Antioxidant activities, polyphenolic composition and their correlation analysis on Hibiscus sabdarifa L. (sabdariffa) calices
محورهای موضوعی : مجله گیاهان دارویی
حکیمه علومی
1
(Department of Ecology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran)
شهریار شاکری
2
(Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran)
مجید بهزادی
3
(Agricultural Research, Education and Extension Organization, Kerman Center, Kerman, Iran)
کلید واژه: Flavonoids, Antioxidant enzymes, Phenolic compounds, anthocyanin, Hibiscus sabdariffa,
چکیده مقاله :
Background & Aim:Extracts of Roselle calyx, possess compounds which could be considered as a great source of natural antioxidants. In this project, antioxidant activities, phenolic compounds content and their correlation analysis on calices of Roselle (Hibiscus sabdariffa) was investigated. Experimental: Total polyphenolic compound, flavonoids and anthocyanins were determined spectrophotometrically. Catalase and peroxidase activity was estimated based on unit per milligram protein. Total antioxidant capacities of the extract were studied using phosphomolybdate assay, DPPH assay and FRAP method. Results: The hibiscus calices were rich in phenolic compounds, flavonoids and anthocyanins. Peroxidase and catalase activity was estimated as 3.13 and 0.05 unit mg-1 protein, respectively. Calyx extract showed high antioxidant activities specially based on DPPH assay. Correlation analysis was also performed between parameters. There was a positive significant correlation between the levels of polyphenolics and flavonoids, anthocyanins and antioxidant capacity of calices at PRecommended applications/industries: Extracts of H. sabdariffa L. (sabdariffa) calices are rich in antioxidant compounds including total phenolic compounds, flavonoids content, and anthocyanins. Based on all the method of measuring antioxidant capacities in this project, the calices showed an acceptable antioxidant activity. Thus, H. sabdariffa L. (sabdariffa) may be suggested as a potential source of natural antioxidants in food and pharmaceutical industry.
مقدمه و هدف: عصاره کاسبرگ چای ترش غنی از ترکیبات آنتی اکسیدانی طبیعی می‌باشد. در این پژوهش بررسی همبستگی فعالیت آنتی اکسیدانی و ترکیبات فنولی گیاه چای ترش مورد بررسی قرار گرفت. روش تحقیق: محتوای ترکیبات فنولی کل، فلاونوئیدها و آنتوسیانین ها بصورت اسپکتروفتومتری بررسی شد. فعالیت آنزیم های کاتالاز و پروکسیداز در میلی گرم پروتئین محاسبه شد. فعالیت آنتی اکسیدانی عصاره بر اساس روش فسفومولیبدات، درصد مهارDPPH و قدرت احیا فریک مورد بررسی قرارگرفت. نتایج و بحث: کاسبرگ چای ترش غنی از ترکیبات فنولی، فلاونوئیدها و آنتوسیانین می‌باشد. فعالیت کاتالاز و پراکسیداز معادل 13/3 و 05/0 واحد در میلی گرم پروتئین بود. بر اساس روش DPPH فعالیت آنتی اکسیدانی عصاره کاسبرگ بسیار بالا می‌باشد. آزمون همبستگی بین پارامترهای مورد بررسی انجام شد. نتایج همبستگی وجود رابطه بین محتوای ترکیبات فنولی، فلاونوئیدها، آنتوسیانین‌ها و ظرفیت انتی اکسیدانی کاسبرگ گیاه چای ترش را در سطح احتمال 5% تایید می‌کند. توصیه‌های کاربردی: عصاره کاسبرگ چای ترش غنی از ترکیبات آنتی اکسیدانی شامل ترکیبات فنولی، فلاونوئیدها و آنتوسیانین می‌باشد. براساس روش های سنجش ظرفت آنتی اکسیدانی، کاسبرگ‌ها فعالیت آنتی اکسیدانی قابل قبولی دارند. بنابراین، عصاره این گیاه بعنوان منبع طبیعی ترکیبات اکسیدان قابل استفاده در صنایع غذایی و داروی گیاهی معرفی می‌گردد.
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