Effect of Boswellia serrata Triana & Planch. gum resin administration during lactation on morphology of pyramidal neurons in hippocampus of rat
محورهای موضوعی : مجله گیاهان دارویی
محمد حسینی شریف آباد
1
(دانشیار گروه بیولوژی و علوم تشریح، دانشگاه علوم پزشکی شهید صدوقی یزد، یزد، ایران؛)
ابراهیم اسفندیاری
2
(استاد گروه زیست پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران؛)
کلید واژه: Dendrites, Cornu ammonis, Frankinsence, Boswellia serrata Triana & Planch,
چکیده مقاله :
Background & Aim: In traditional medicine, Boswellia species gum resin known as Frankincense or Olibanum, has been administrated in elderly for enhancement of memory and also in pregnant women to increase memory and intelligence of progeny. However, it has been rarely scientifically documented so far. We have previously reported that maternal administration of Frankincense during lactation increased learning and memory performance. In this study, using animal model of rat, we investigated the morphological changes of pyramidal neurons of hippocampus, an important brain centre involved in learning and memory, following maternal administration of Frankincense during three week of lactation. Experimental: Two months-old male Wistar rats whose mothers were given by gavage the aqueous extract of the B. serrata (0.100 mg/kg/day) during lactation were deeply anesthetized and transcardially perfused with fixative solution. Each brain was then removed from skull and divided into two hemispheres. The right hemispheres were selected for morphometerical study. Transverse sections (100 μm thick) were cut through the entire extent of the hippocampus. In a Golgi study, the number of dendritic branching and segments was counted by Sholl’s method. Results & Discussion: Statistical analysis of data indicated that administration of Frankincense during lactation increase the number of dendritic segments, branching of Cornu Ammonis (CA) pyramidal cells in the hippocampus of rat offspring. There was no significant difference in the dendritic length of CA pyramidal cells between experimental and control groups. These results suggest that maternal administration of Frankincense during lactation increase dendritic arborization in the hippocampus. It can be concluded that such morphological changes leads to an increase in the synaptic contacts and it provides one of the bases for the memory improvement induced by Frankincense administration. Industrial/Practical recommendation: Our findings suggest that Boswellia resin and its active ingredients to be extensively studied in neurophysiology. It also suggests that compounds from Boswellia resin may be used in treatment of neurodegenerative disorders.
مقدمه و هدف: کندر صمغ گونه Boswellia serrata است که طی سالیان دراز در طب سنتی برای افزایش حافظه افراد مسن و نیز مادران باردار برای افزایش هوش و حافظه فرزندان خود تجویز می شده است. با این حال تاکنون مستندات علمی اندکی در این رابطه موجود است. در این مطالعه با استفاده از مدل حیوانی موش بزرگ آزمایشگاهی(رت)، تغییرات مورفولوژیکی نورونهای هیپوکامپوس که مرکز مهم مغزی در روند یادگیری و حافظه می باشد متعاقب تجویز مادری کندر در سه هفته دوره شیردهی بررسی شد. روش تحقیق: موشهای صحرایی نر دو ماهه نژاد ویستار که به مادران آنها در دوره شیردهی بهمدت سه هفته بهصورت روزانه mg/kg100 عصاره آبی کندر خورانده شده بود را عمیقاً بیهوش کرد و با تزریق محلول ثابت کننده از راه قلب، مغز را ثابت نموده و از جمجمه خارج گردید. سپس آن را به دو نیمکره تقسیم نموده و نیمکره راست برای مطالعه مورفومتری انتخاب و مقاطع عرضی از کل هیپوکامپوس بریده شد. تعداد انشعابات و قطعات دندریتی با استفاده از روش Sholl شمرده شد. نتایج و بحث: تحلیل آماری دادهها نشان داد که تجویز کندر در دوره شیردهی تعداد قطعات و انشعابات دندریتی سلولهای هرمی شاخ آمون هیپوکامپوس موالید رت را افزایش می دهد. اما تفاوت معنی داری از لحاظ طول دندریت سلولهای هرمی در گروههای آزمایش و شاهد وجود نداشت. بر اساس این یافته ها پیشنهاد میشود که تجویز مادری کندر در دوره شیردهی، شاخه های دندریتی در هیپوکامپوس را افزایش میدهد و میتوان نتیجه گرفت که چنین تغییرات مورفولوژیکی منجر به افزایش تعداد تماسهای سیناپسی میشود و این یکی از مبانی افزایش حافظه به دلیل تجویز کندر را فراهم می کند. توصیه کاربردی /صنعتی: با توجه به یافته های این مطالعه و سایر مطالعات پیشین ما مبنی بر تأثیر مصرف کندر در فراهم نمودن بستر مورفولوژیکی لازم برای افزایش قوای یادگیری و حافظه پیشنهاد می گردد این ماده و مشتقاتش در تحقیقات نوروفیزیولوژیک مورد ارزیابی بیشتر قرار گیرد و نتایج آن در پیشگیری یا بهبود بیماریهای دژنراتیو عصبی که منجر به فراموشی می شود استفاده شود.
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