Antimicrobial activity of Prosopis farcta L. and Datura stramonium L. extracts against Staphylococcus aureus
محورهای موضوعی : مجله گیاهان داروییسعیده سعیدی 1 , نفیسه مهدی نژاد 2 , مجید شریفی راد 3 , فرشته جوادیان 4 , الهام جوادیان 5
1 - مرکز تحقیقات گیاهان دارویی زابل،دانشگاه علوم پزشکی زابل، زابل، ایران
2 - گروه اصلاح نباتات و بیوتکنولوژی، دانشگاه زابل، زابل، ایران
3 - گروه مدیریت آبخیزداری، دانشگاه زابل، زابل، ایران
4 - مرکز تحقیقات گیاهان دارویی زابل،دانشگاه علوم پزشکی زابل، زابل، ایران
5 - مرکز تحقیقات گیاهان دارویی زابل،دانشگاه علوم پزشکی زابل، زابل، ایران
کلید واژه: Staphylococcus aureus, plant extract, Prosopis farcta, Datura stramonium,
چکیده مقاله :
Background & Aim: The aim of this study was to investigate the antimicrobial activity of two rangeland-medical plants extracts, Prosopis farcta L. and Datura stramonium L., against Staphylococcus aureus isolated from sheep in Zabol city. Experimental: The P. farcta and D. stramonium were collected from the rangelands of Zabol city. Staphylococcus aureus strains were isolated from the nose of sheep in Zabol city. Antibiotic resistance pattern was determined by Kirby Bauer method. Finally, the minimum inhibitory concentration (MIC) and minimum bactericide concentration (MBC) were determined by microdilution method. Results: The results of this study showed that the P.farcta extract had a MIC of 25 ppm, and 10 strains of the bacterium were inhibited in this concentration. The lowest inhibitory concentration of D.stramonium extract was 6.25 ppm, and one strain was inhibited in this concentration. On the other hand, the highest inhibitory concentration was 50 ppm and the highest bactericide concentration was 100 ppm. Recommended applications/ industries: The results of this study showed good antimicrobial effects of D. stramonium and P. farcta extracts that can be used to treat Staphylococcus aureus infections.
مقدمه و هدف: هدف از این مطالعه بررسی فعالیت ضد میکروبی عصاره دو گیاه دارویی جغجغه و تاتوره بر روی استافیلوکوکوس اورئوس جدا شده از بینی گوسفندان در شهرستان زابل است. روش تحقیق: گیاه تاتوره و جغجغه از مناطق شهر زابل جمع آوری شد. سویه های استافیلوکوکوس اورئوس از بینی گوسفندان شهرستان زابل جمع آوری شد. الگوی مقاومت آنتی بیوتیکی با روش کربی بائر تعیین شد. حداقل غلظت مهار کنندگی و حداقل غلظت کشندگی با روش میکرودایلوشن تعیین شد. نتایج: نتایج مطالعه حاضر نشان داد که حداقل غلظت مهار کنندگی عصاره جغجغه برابر با ppm25 بوده است و 10 سویه در این غلظت مهار شده اند. کمترین غلظت مهار کنندگی عصاره تاتوره ppm 6.25 بوده و یک سویه در این غلظت مهار شد. بیشترین غلظت مهار کنندگی برابر با ppm 50 و بیشترین غلظت کشندگی ppm 100 مشاهده شد. توصیه های کاربردی/ صنعتی: نتایج این مطالعه مبنی بر اثر ضد میکروبی خوب عصاره گیاه تاتوره و جغجغه نشان داد که میتواند برای درمان عفونت های استافیلوکوکی استفاده شود.
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