Ameliorative potentials of Aju Mbaise extract (AME) on Dutasteride induced oxidative stress and hepatic injury in rats
محورهای موضوعی : مجله گیاهان دارویی
Robert Uroko
1
,
Elisha Ogwo
2
,
Paul Nweje-Anyalowu
3
,
Ikenna Obiwuru
4
,
Chinomso Aaron
5
,
Obinna Mba
6
1 - Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria;
2 - Department of Human Physiology, College of Medicine and Health Sciences, Abia State University, Uturu, Nigeria;
3 - Department of Biochemistry, Faculty of Science, Clifford University, Owerrinta, Abia State, Nigeria;
4 - Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria;
5 - Department of Biochemistry, Faculty of Biological and Physical Sciences, Abia State University, Uturu, Nigeria;
6 - Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, Abia State, Nigeria;
کلید واژه: Oxidative stress, antioxidants, Aju Mbaise, Dutasteride, Hepatic injury, Liver markers,
چکیده مقاله :
Background & Aim: Aju Mbaiseis a polyherbal extract with nutraceuticalproperties that helps to replenish the volume of blood lost during childbirth and improves breast milk secretion and the general wellbeing of the mother. This study evaluated the ameliorative potentials of Aju Mbaise extract (AME) on Dutasteride-induced oxidative stress and hepatic injury in rats. Twenty-one rats were used to assess the acute toxicity of AME.Experimental: The study for the hepatoprotective effects of AME had five groups of rats, including normal control, Dutasteride only, AME only, Dutasteride + AME (500 mg/kg) and Dutasteride+ AME (1000 mg/kg).Results: The acute toxicity result showed that AME is relatively safe for consumption. Dutasteride caused significant elevation of liver marker enzymes, including aspartate transaminase (AST), alanine transaminase (ALT), transaminase (AST), alkaline phosphatase (ALP), total bilirubin, malondialdehyde (MDA) and significantly reduced catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), total proteins, albumin, and globulin levels in the rats received only Dutasteride. In contrast, Dutasteride induced rats treated with AME showed a significant decline in the AST, ALT, ALP, MDA, and bilirubin and significantly increased SOD, GSH, GPx, total proteins, albumin, and globulin levels compared to Dutasteride induced untreated rats. The AME-treated rats showed normal liver histo-architecture, unlike the Dutasteride-induced untreated rats that showed mild to moderate vacuolar degeneration of the hepatocytes.Recommended applications/industries: The findings show that AME ameliorates Dutasteride caused rats oxidative stress and hepatic injury.
Background & Aim: Aju Mbaiseis a polyherbal extract with nutraceuticalproperties that helps to replenish the volume of blood lost during childbirth and improves breast milk secretion and the general wellbeing of the mother. This study evaluated the ameliorative potentials of Aju Mbaise extract (AME) on Dutasteride-induced oxidative stress and hepatic injury in rats. Twenty-one rats were used to assess the acute toxicity of AME.Experimental: The study for the hepatoprotective effects of AME had five groups of rats, including normal control, Dutasteride only, AME only, Dutasteride + AME (500 mg/kg) and Dutasteride+ AME (1000 mg/kg).Results: The acute toxicity result showed that AME is relatively safe for consumption. Dutasteride caused significant elevation of liver marker enzymes, including aspartate transaminase (AST), alanine transaminase (ALT), transaminase (AST), alkaline phosphatase (ALP), total bilirubin, malondialdehyde (MDA) and significantly reduced catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx), reduced glutathione (GSH), total proteins, albumin, and globulin levels in the rats received only Dutasteride. In contrast, Dutasteride induced rats treated with AME showed a significant decline in the AST, ALT, ALP, MDA, and bilirubin and significantly increased SOD, GSH, GPx, total proteins, albumin, and globulin levels compared to Dutasteride induced untreated rats. The AME-treated rats showed normal liver histo-architecture, unlike the Dutasteride-induced untreated rats that showed mild to moderate vacuolar degeneration of the hepatocytes.Recommended applications/industries: The findings show that AME ameliorates Dutasteride caused rats oxidative stress and hepatic injury.
Abdulmaged, T., Haider, K.S., Gheorghe, D. and Ahmad, H. 2017. Long-term Dutasteride therapy in men with benign prostatic hyperplasia alters glucose and lipid profiles and increases the severity of erectile dysfunction. Hormone Molecular Biology and Clinical Investigation, 30(3): 20170015.
Abou-Seif, H.S., Hozayen, W.G. and Hashem, K.S. 2019. Thymus vulgaris extract modulates dexamethasone-induced liver injury and restores the hepatic antioxidant redox system. Beni-Suef University Journal of Basic and Applied Sciences, 8:21-29.
Adewole, K.E. and Adebayo, J.O. 2017. Antioxidant defence system induced by cysteine stabilised peptide fraction of aqueous extract of Orinda lucida leaf in selected tissues of Plasmodium berghei-infected mice. Journal of Integrative Medicine, 15:388-397.
Aebi, H. 1984. Catalase in vitro. Methods in Enzymology, 105: 121-126.
Doumas, B.T. and Biggs, H.G. 1972. Determination of Serum Albumin. In: Standard Methods of Clinical Chemistry. Cooper GA, Ed. NY Academic Press Inc. pp. 7:175.
Gornall, A.G., Bardawill, J.C. and David, M.M. 1949. Determination of serum proteins by means of Biuret reaction. Journal of Biological Chemistry, 177: 751-760.
Ijioma, S.N., Emmanuel, O., Nosiri, C.I. and Ugbogu, E.A. 2021. Evaluation of toxicity profile and pharmacological potentials of Aju Mbaise polyherbal extract in rats. Scientific African, 11 (2021) e00681.
Ijioma, S.N., Osim, E.E., Nwankwo, A.A., Nwosu, C.O., Nwankudu, O.N. and Ekeleme, C.M. 2017. Antioxidant potentials and effects on the haematology and osmotic fragility scores of a polyherbal formulation used in Southeast Nigeria. Journal of Basic and Clinical Physiology and Pharmacology, 2017: 1-10
Jendrassik, L. and Grof, P. 1938. Simplified Photometric: Methods for the determination of bilirubin. Biochemical Journal, 297: 81-89.
Kakkar, P., Das, B. and Viswanathan, P.N. 1984. A modified spectrophotometric assay of superoxide dismutase. Indian Journal of Biochemistry and Biophysics, 21(2):130-132.
Kim, S.M., Diao, W.J., An, W., Kim, H.J., Lim, H.J., Kim, K.N., Bae, G.W. and Kang, J.S. 2022. Effect of porcine placental extract mixture on alcohol-induced hepatotoxicity in rats. Current Issues in Molecular Biology, 44: 2029-2037.
Kurczewski, R., Bowen, C., Collins, D., Zhu, J., Serbest, G. and Manyak, M. 2017. Bioequivalence studies of a reformulated dutasteride and tamsulosin hydrochloride combination capsule and a commercially available formulation. Clinical Pharmacology in Drug Development, 6: 508-516.
Livingstone, D.E., Barat, P., Di Rollo, E.M., Rees, G.A., Weldin, B.A. and Rog-Zielinska, E.A. 2015. 5α-Reductase type 1 deficiency or inhibition predisposes to insulin resistance, hepatic steatosis, and liver fibrosis in rodents. Diabetes, 64:447-58.
Lorke, D. 1983. A new approach to practical acute toxicity testing. Archives of Toxicology, 54: 275-87.
Mohamad, B.J., Obaid, H.H., Mohamad, D.I. and Salim, M. 2017. Study the toxic effect of different doses of duprost in liver and blood of albino mice. Iraqi Journal of Science, 58: 1381-1392.
Ogueke, C.C., Clifford, I., Owuamanam, C.O., Iroanya, A., Bede, E.N. and Nwachukwu, I.N. 2016. Antibacterial activity, phytochemical properties and mineral content of Aju Mbaise decoction: a liquid extract administered to nursing mothers. Nigerian Journal of Nutritional Sciences, 37: 114–120.
Olayinka, E.T., Ore, A. and Adeyemo, O.A. 2015. Quercetin, a flavonoid antioxidant, ameliorated procarbazine-induced oxidative damage to murine tissues. Antioxidants, 4: 304-321.
Roehrborn, C.G., Siami, P., Barkin, J., Damião, R., Major-walker, K., Morrill, B. and Montorsi, F. 2008. The effects of Dutasteride, tamsulosin and combination therapy on lower urinary tract symptoms in men with benign prostatic hyperplasia and prostatic enlargement: 2-year results from the combat study. Journal of Urology, 179: 616-621.
Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G. and Hoekstra, W.G. 1973. Selenium: Biochemical role as a component of glutathione peroxidase. Science, 179(4073): 588-590.
Subramaniam, S., Khan, H.B.H., Elumalai, N. and Lakshmi, S.Y.S. 2015. Hepatoprotective effect of ethanolic extract of whole plant of Andrographis paniculata against CCl4-induced hepatotoxicity in rats. Comparative Clinical Pathology, 24: 1245-1251.
Tilahun, T.J.M.M. 2018. Ethnobotanical study of medicinal plants used to treat human diseases in Berbere District, Bale Zone of Oromia Regional State, South East Ethiopia. Evidence-Based Complementary and Alternative Medicine, 18(5): 2-15.
Traish, A., Haider, K., Doros, G. and Haider, A. 2017. Long-term dutasteride therapy in men with benign prostatic hyperplasia alters glucose and lipid profiles and increases severity of erectile dysfunction. Hormone Molecular Biology and Clinical Investigation, 30: 1-16.
Uroko, R.I., Sangodare, R.S.A., Muhammad, K.H. and Asadu, C.L. 2015. Effect of methanol extract of Abrus precatorius leaves on male Wistar albino rats induced liver damage using carbon tetrachloride. Journal of Biological Sciences, 15 (3): 116-123.
Uroko, R.I., Agbafor, A., Egba, S.I., Nwuke, C.P. and Kalu-Kalu, S. 2021. Evaluation of antioxidant activities and haematological effects of Asystasia gangetica leaf extract in monosodium glutamate-treated rats. Lekovite Sirovine, 41: 5-11.
Uroko, R.I., Nweje-Anyalowu, P.C., Aaron, C.F. and Chukwu, C.N. 2022. Combined extract of Spermacoce radiata (DC.) Hiern and Hypselodelphys poggeana (K. Schum.) Milne-Redh leaves (CEESH) confer hepatoprotection in rat induced benign prostatic hyperplasia. Plant Biotechnology Persa, 4(1): 78-88.
Wallin, B., Rosengren, B., Shertzer, H.G. and Camejo, G. 1993. Lipoprotein oxidation and measurement of thiobarbituric acid reacting substances formation in a single microtiter plate: Its use for evaluation of antioxidants. Analytical Biochemistry, 208(1):10-15.
Yahya, F., Sfouq, F., Mahmoud, A.Z. and Nayyar, N. 2018. Chemical composition and antimicrobial, antioxidant, and anti-inflammatory activities of Lepidium sativum seed oil. Saudi Journal of Biological Sciences, 3(1): 2-5.
Zhu, R., Wang, Y., Zhang, L. and Guo, Q. 2012. Oxidative stress and liver disease. Hepatology Research, 42: 741-749.
