Effect of ellagic acid on oxidative stress duo to brain ischemia/hypoperfusion in male rat
محورهای موضوعی : مجله گیاهان دارویی
مریم رفیعی راد
1
,
خدیجه قاسم زاده دهکردی
2
1 - گروه زیست شناسی دانشگاه ازاد اسلامی واحد ایذه، خوزستان
2 - گروه زیست شناسی، دانشگاه آزاد اسلامی واحد سنندج
کلید واژه: Malondialdehyde, Oxidative stress, thiol, ellagic acid, Brain Ischemia,
چکیده مقاله :
Background & Aim: Free radicals are produced in ischemic processes. Nerve damage caused by free radicals may play a role in neurological diseases and antioxidants are protective activity. Ellagic acid is a polyphenol compound with antioxidant properties which is found in fruits like pomegranate, blackberry, and all types of mulberry. This study aimed to evaluate the effect of 14 days of oral administration of ellagic acid (50 mg/kg) on brain oxidative stress indices after permanent bilateral common carotid artery occlusion or ischemia/hypoperfusion in male adult rats. Experimental: A number of 30 rats were divided into test and control groups. To make animal model of permanent cerebral hypo perfusion/ischemia, carotid arteries were ligatured as upper and lower and cut bilaterally. Rat brain tissue was extracted to separate hippocampus and measure malondialdehyde, and thiol (-Sh) groups. Results & Discussion: Our results indicated that malondialdehyde and thiol in ischemia group has significantly increased (p < 0.001) than to control group. In addition, the rate of malondialdehyde and thiol in ischemia group receiving the ellagic acid has significantly decreased (p < 0.001), than to ischemia group. Recommended applications/industries: Ellagic acid possibly with powerful antioxidant properties, can improve the effect of ischemia such as production of free radicals.
مقدمه و هدف: رادیکال های آزاد در طی فرآیندهای ایسکمی تولید می شوند. رادیکال های آزاد در بیماری های عصبی نقش دارند و آنتی اکسیدان ها نقش محافظتی دارند. الاژیک اسید یک ترکیب پلی فنلی با خواص آنتی اکسیدانی است که در میوه هایی مانند انار، زغال اخته و انواع توت یافت می شود. این مطالعه با هدف بررسی اثر 14 روز تجویز خوراکی الاژیک اسید 50 میلی گرم بر کیلوگرم بر شاخص های استرس اکسیداتیو مغز پس از انسداد دائمی دو طرفه شریان کاروتید مشترک و یا ایسکمی/ هیپوپرفوزیون در موش‌های صحرایی نر بالغ انجام‌گرفت. مواد و روش‌ها: تعداد 30 سر موش به گروه های آزمون و کنترل تقسیم شدند. برای ایجاد ایسکمی در موش صحرایی، شریان های کاروتید عمومی بوسیله ابزارهای بخیه ی پوستی با دو گره محکم در حول رگ (بالا و پایین) مسدود و سپس شریان ها به طور کامل از وسط قطع گردیدند. سپس مغز موش‌ها جهت ارزیابی میزان پراکسیداسیون لیپیدی و اندازه گیری میزان گروه های تیول(-SH) استخراج شدند. نتایج و بحث: نتایج تحقیق حاضر نشان داد که میزان مالون دی آلدئید و تیول در گروه ایسکمی نسبت به گروه کنترل افزایش معنی داری داشته است و میزان مالون دی آلدئید و تیول در گروه ایسکمی دریافت کننده الاژیک اسید نسبت به گروه ایسکمی کاهش معنی داری داشت. توصیه کاربردی/صنعتی: الاژیک اسید با خاصیت آنتی اکسیدانی قوی توانسته‌است اثرات ایسکمی ازجمله تولید رادیکال‌های آزاد را بهبود ببخشد.
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