Antibacterial Effects of Methanolic Extract of Myristica fragrans against Klebsiella pneumoniae and Acinetobacter baumannii producing Broad-Spectrum beta-lactamase
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
الهام نیکویی
1
,
اشرف کریمی نیک
2
1 - گروه میکروبیولوژی ، واحد کرمان ، دانشگاه ازاد اسلامی ، کرمان، ایران
2 - گروه میکروبیولوژی، واحد کرمان، دانشگاه آزاد اسلامی،کرمان،ایران
کلید واژه: Antibacterial activity, Klebsiella pneumoniae, Acinetobacter baumannii, beta-lactamase, Myristica fragrans.,
چکیده مقاله :
The increase in various strains of antibiotic-resistant bacteria have become one of the primary concerns. Therefore,
efforts to use plant-derived drugs against drug-resistant bacteria have gained special importance. The
aim of this study was to determine the antibacterial effects of methanol extract of Myristica fragrans against
Klebsiella pneumoniae and Acinetobacter baumannii isolates producing broad-spectrum beta-lactamases. The
plant extract was prepared using the maceration method. Then, the extract was filtered through Whatman filter
paper No.1 one and concentrated and dried using a rotary evaporator system. Concentrations of 80, 40, 20,
10, 5, 2.5, 1.25, and 0.625 mg/mL of the extract were prepared in dimethyl sulfoxide and methanol (1:1v/v) as
solvents. The identification of beta-lactamase-producing isolates was carried out using the phenotype method
with antibiotic of cefotaxime and the combination of cefotaxime/clavulanic acid. The antibacterial activity
against the isolates was investigated using the agar well diffusion method. Based on the results, 33% of the
Klebsiella isolates and 50% of the Acinetobacter isolates were found to be beta-lactamase producers. All of
the isolates were sensitive to the methanol extract of Myristica fragrans, with the average minimum inhibitory
concentration 10 and 5 mg/mL, respectively. According to the findings, it can be inferred that the Myristica
fragrans extract has In vitro the ability to Klebsiella and Acinetobacter isolates. Therefore, with further research
and identification of active compounds, it may be possible to use this extract as an alternative antibiotic
for treatment in the future.
The increase in various strains of antibiotic-resistant bacteria have become one of the primary concerns. Therefore,
efforts to use plant-derived drugs against drug-resistant bacteria have gained special importance. The
aim of this study was to determine the antibacterial effects of methanol extract of Myristica fragrans against
Klebsiella pneumoniae and Acinetobacter baumannii isolates producing broad-spectrum beta-lactamases. The
plant extract was prepared using the maceration method. Then, the extract was filtered through Whatman filter
paper No.1 one and concentrated and dried using a rotary evaporator system. Concentrations of 80, 40, 20,
10, 5, 2.5, 1.25, and 0.625 mg/mL of the extract were prepared in dimethyl sulfoxide and methanol (1:1v/v) as
solvents. The identification of beta-lactamase-producing isolates was carried out using the phenotype method
with antibiotic of cefotaxime and the combination of cefotaxime/clavulanic acid. The antibacterial activity
against the isolates was investigated using the agar well diffusion method. Based on the results, 33% of the
Klebsiella isolates and 50% of the Acinetobacter isolates were found to be beta-lactamase producers. All of
the isolates were sensitive to the methanol extract of Myristica fragrans, with the average minimum inhibitory
concentration 10 and 5 mg/mL, respectively. According to the findings, it can be inferred that the Myristica
fragrans extract has In vitro the ability to Klebsiella and Acinetobacter isolates. Therefore, with further research
and identification of active compounds, it may be possible to use this extract as an alternative antibiotic
for treatment in the future.
